Determination of antibody levels to hepatitis B surface antigen (HBsAb) in Shahrekord Hajar hospital staffs, 2007-8

زمینه و هدف: ویروس هپاتیتB یکی از عوامل ایجاد کننده هپاتیت های ویروسی بوده و سیروز کبدی یکی از عوارض مهم آن می باشد. کارکنان بیمارستان یکی از گروه های پر خطر ابتلا به این بیماری می باشد که لزوم واکسیناسیون در آنها توصیه شده است. این مطالعه با هدف اندازه گیری سطح سرمی هپاتیت B در کارکنان بیمارستان هاجر(س)، انجام شد. روش بررسی: در این مطالعه توصیفی - تحلیلی، سطح آنتی بادی ویروس هپاتیت B در سرم 257 نفر از کارکنان بیمارستان هاجر(س) شهرکرد در سال 87-1386 که همگی واکسینه شده بودند با استفاده از تکنیک الایزا تعیین گردید. داده های بدست آمده به کمک آزمون های آماری کای دو تجزیه و تحلیل شد. یافته ها: از 257 نفر مورد بررسی، 21 نفر (2/8) فاقد ایمنی، 85 نفر (33) ایمنی نسبی و 151 نفر (8/58) ایمنی کامل داشتند. در افراد واکسینه شده بین سطح ایمنی و مدت زمان سپری شدن از آخرین نوبت واکسیناسیون ارتباط معنی داری بدست آمد (05/0

The effect of deep-slow and regular breathing on pain intensity of burn dressing

Aims: Burn pain is one of the severest and most consistent types of pain. Patients who suffer from burn usually experience the highest level of pain at the time of dressing change. The aim of the present study is “determining the effect of deep-slow and regular breathing on pain intensity of dressing in patients with burns”. Methods: This study is a randomized clinical trial in two intervention and control groups which investigate the pain in 68 patients referring to the burn ward of Kashani hospital of Shahrekord in the period of March 2011 to August 2011 through random and convenient sampling, these patients were in one of intervention or control group. Data were collected with visual analogue scale and analyzed by using SPSS, descriptive statistic test, independent t-test and chi-square. Results: At first the mean of dressing pain intensity score before and after intervention in intervention group was 5.82, 3.66 and in control group was 5.3, 4.8 respectively. There was significant difference between the pain intensity after dressing in intervention and control groups (p=0.04). Conclusions: Using deep-slow and regular breathing can be an important factor in reducing pain intensity during burn dressing

Heparin effects on mobility problems of non-hemorrhagic stroke patients

Stroke is the second leading cause of death worldwide. Ischemia is the most common cause of it which is being treated by combined therapy. One important management of acute stroke candidate for recanalization (r-TPA) that can perform for some patient with special condition in 3 to 4/5hours of onset in a few centers in Iran. there are many studies with different results regard to anti-coagulant therapy in acute stroke. The aim of this study was determination of heparin effects on mobility problems among non-hemorrhagic stroke patients. In regard of absence of diagnostic tools & teams of acute ischemic stroke treatments with r-TPA. In a double-blind randomized controlled clinical trial, 60 non-hemorrhagic stroke patients in Kashani Hospital in Shahrekord were randomly assigned in according to scale definition of NIHSS(part 5 & 6 : motor arms & legs) into two groups with same motor signs(0=no drift -1=drift -2 = cant's resist gravity-3 = no efforts against gravity-4 = no movement UN=untestable): experiment and control groups. While experiment group were subcutaneous received 5000 to 10000 unit BID every day for 3 days +aspirin 100-325 mg, control group were received only 100-325 mg aspirin. Muscular power and dyspnea & pulses of peripheral veins for evaluation of lung emboli & DVT and radiological data in CT(the first and third days) were evaluated after 3 days in two groups. There was no statistically significant difference between two groups in age, gender, power of all limbs, and duration of hospitalization. There was no significant difference between two groups in muscular power of upper and lower limbs in first day, but it was significant in the third day. In comparison of the muscular power of limbs of patients less than 55 years between two groups, there was no significant difference in the first day; however, it was significant in the third day. For patients more than 55, the significant difference was seen only in the third day in the power of left side limbs. This study recommends using heparin in non-hemorrhagic stroke patients which is more efficient than using only aspirin. Jivad N, Moghni M, Azari Beni A, Shahrifar M, Azimian M. Heparin effects on mobility problems of non-hemorrhagic stroke patients. Life Sci J 2012;9(4):5601-5604] (ISSN: 1097-8135). http://www.lifesciencesite.com. 83

Study of Linc00574 Regulatory Effect on the TCTE3 Expression in Sperm Motility

We investigated the relationship of t-complex-associated-testis-expressed 3 (TCTE3) and linc00574 expression levels with sperm motility and morphology in patients with asthenozoospermia (AZ) and terato-asthenozoospermia (TAZ). The study population consisted of 31 AZ patients, 31 TAZ patients, and 32 normozoospermia (NZ) as controls. Quantitative real-time PCR was conducted to evaluate the expression levels of TCTE3 and linc00574. Bioinformatics investigations were performed using databases to find molecular pathway. TCTE3 expression was reduced significantly in AZ and TAZ patients (P < 0.05). Linc00574 expression level increased only in the AZ patients (P < 0.05). The subsequent analyses showed a significantly positive correlation between TCTE3 and linc00574 expression levels (P < 0.05). In addition, a significantly positive relationship was observed between TCTE3 expression level and sperm motility and morphology (P < 0.05). The present study suggests that TCTE3 expression is regulated by linc00574 through a negative self-regulating mechanism and therefore may affect the flagella structure and functio

Study of Linc00574 Regulatory Effect on the TCTE3 Expression in Sperm Motility

We investigated the relationship of t-complex-associated-testis-expressed 3 (TCTE3) and linc00574 expression levels with sperm motility and morphology in patients with asthenozoospermia (AZ) and terato-asthenozoospermia (TAZ). The study population consisted of 31 AZ patients, 31 TAZ patients, and 32 normozoospermia (NZ) as controls. Quantitative real-time PCR was conducted to evaluate the expression levels of TCTE3 and linc00574. Bioinformatics investigations were performed using databases to find molecular pathway. TCTE3 expression was reduced significantly in AZ and TAZ patients (P < 0.05). Linc00574 expression level increased only in the AZ patients (P < 0.05). The subsequent analyses showed a significantly positive correlation between TCTE3 and linc00574 expression levels (P < 0.05). In addition, a significantly positive relationship was observed between TCTE3 expression level and sperm motility and morphology (P < 0.05). The present study suggests that TCTE3 expression is regulated by linc00574 through a negative self-regulating mechanism and therefore may affect the flagella structure and function

Parotid swelling due to Hoshino strain following MMR vaccination in iranian immunization program

This paper reports two seronegative cases of parotid swelling following MMR vaccination, which contains Hoshino strain of mumps virus. This study showed a high,(5%) potential reactogenicity induced by Hoshino strain of current MMR vaccine administered in Iranian EPI. The rate of complication of parotid swelling following national immunization against mumps is more than natural infection. Based on the results of this report, there is the first report of occurrence of parotid swelling 31 days following MMR vaccination. This

IMMUNOGENICITY AND EFFICACY OF THE HOSHINO STRAIN OF MUMPS (INCLUDED IN MMR VACCINE) AGAINST MUMPS IN SHAHR-E-KORD, IRAN

This report describes the immunogenicity and efficacy and long term immunity of Hoshino strain of Mumps (included in MMR Vaccine) in shahr-e-kord, Islamic Republic of Iran (I.R.Iran). A total of 338 Children aged 3-18 years were tested for Mumps IgG using enzyme-linked immunosorbent assay (ELISA). The proportion of susceptible, mumps IgG negative, children was 19.8% (67 subjects). Of the 67 susceptible children, 36 received the MMR vaccination and successfully completed the study. Blood was collected by venipuncture 3, 12, and 24 months after vaccination and serum samples were tested by ELISA for detection of Mumps IgM and IgG. The overall seroconversion rate was 86.1%, 77.7% and 75% at 3, 12, and 24 months after vaccination respectively

Association between H. pylori BabA virulence factor with clinical outcome and ABO blood groups

Helicobacter pylori infection is a prevalence infection 50% of the human population. The main H. pylori adhesin is the BabA, which was the first identified factor. The aim of this study was to determine the relationship between the ABO blood groups and various gastrointestinal diseases 140 patients, were included in this study. Gastric biopsies were taken for recognition of H.pylori by RUT and PCR. Blood samples were tested for ABO blood group. In the present study 140 H.pylori positive samples examined for the presence or absence of babA gene by PCR. From 140 patients, 35% were positive for babA gene and 65% were negative for this gene. Positivity rate of H. pylori babA infection was 42.4 % in blood group O, 18.8 % in blood group A, 100% in blood group B and 44.8 % in blood group AB. The frequencies of ABO blood group among endoscopic findings are predominant for Gastritis for group A. In our study, There was statistically significant difference in babA (+) and babA (–) were compared in endoscopy finding (P<0.001) and there was statistically significant difference in positivity rate of H.pylori infection among ABO blood groups (p< 0.001). The higher incidence of Gastritis and peptic ulcer was in patients with blood group A and AB and there was statistically significant between these symptoms (p= 0.02). Our results showed that the prevalence of babA genotype is associated with gastritis and gastric ulcer and there is a relation with ABO blood group

Prevalence of cagA and babA2 genes in Helicobacter Pylori strains Isolated from Iranian gastrointestinal disorder patients and their gas-tritis classification

Helicobacter pylori is a spiral gram negative flagellate bacteria and localize in the stomach. H.p infection is a worldwide health problem and identified as an important cause of gastritis and gastric cancer and its ability to develop such disorders is related to its virulence factors and environment. cagA is the most important Hp virulence factor that directly penetrate into gastric epithelial cells by bacterial secretion system (T4SS) from pathogenicity island (PAI) and disrupts cell homeostasis. Adherence factors are significant for bacterial colonization and suitable function of other virulence factor. Blood group antigen binding adhesion (babA) is an outer membrane protein (OMP) that binds to ABO blood group antigen and can stimulate inflammatory response in gastric cells. Our main target was to determine the roles and prevalence of cagA and babA2 virulence factor in gastrointestinal disorders in Iranian patients. Existences of These factors were determined by PCR in 218 patients with gastrointestinal disorders. Semi-quantitative methods of scoring according to the Updated Sydney classification system were used for detection of H.pylori density, neutrophil and monocyte cells infiltration. A high prevalence of cagA positive (81.4%) and babA2 positive (35%) were found. The most combined genotype (cagA&babA2) prevalence was found in gastritis & ulcer (100%) (P < 0.001). High prevalence of cagA positive observed in active inflammation phase 76.9% and high prevalence of babA2 positive was in active phase 61.1% of H.pylori gastritis (P=0.001) . Results of this study showed information about the high prevalence of cagA genes in H.pylori infected patients and their rolls in active gastrointestinal disorder

Local Expression of Mucosal YKL-40; Correlation of YKL-40 with Clinical Manifestations and Immunopathogenesis of Moderate/Severe Persistent Allergic Rhinitis Patients

YKL-40 is an important protein that plays a critical role in chronic inflammation in hypersensitivity disease. In this study, the expression of YKL-40 was investigated among patients with moderate/severe persistent allergic rhinitis (M/S PAR), patients with mild (M) PAR and healthy individuals. Moreover, the association between YKL-40 and immunopathogenesis of M/S PAR was meticulously surveyed. For this purpose, surgical samples were tested by real-time polymerase chain reaction to evaluate YKL-40 mRNA expression. The presence and location of YKL-40 protein in the tissue samples were determined by immunohistochemistry. Additionally, we measured the number of eosinophils per field in the tissue samples, blood eosinophils, total serum IgE, specific serum IgE, total nasal syndrome score (TNSS) and YKL-40 serum levels. The data indicated that production of YKL-40 in patients with M/S PAR increased significantly when compared with the control group. Furthermore, local production of YKL-40 correlated with specific IgE, nasal eosinophil count and TNSS. The results of the present study indicate that YKL-40, for its correlation with allergic clinical manifestations and symptom severity in M/S PAR patients, should be considered as a trigger factor in AR