The building blocks for cardiac repair : isolation and differentiation of progenitor cells from the human heart

Unfortunately cardiovascular disease is very common, which makes it important to gain a better understanding of how we can restore the 'broken' heart after injury . Progenitor cells can serve as the 'building blocks ' for cardiac repair. Cardiac progenitor cells, which are the focus of t his thesis, can be derived from either the myocardial (CPCs) or epicardial (EPDCs) layer of the heart. Based on the idea that we want to isolate CPCs and EPDCs from the human heart and eventually use these cells for cardiac regeneration, in this thesis the f ollowing questions were investigated: • Which marker scan recognize CPCs within human cardiac tissue and how can we efficiently isolate EPDCs? ( Pa r t 1) • Which factors play a role in CPC and EPDC behavior? (Part 2) In Chapter 3, 4 and 5 novel antibodies for the isolation of CPCs are introduced and a new method to isolate and expand EPDCs is presented in Chapter 6. We studied human CPC to cardiomyocyte differentiation in Chapter 7 and questioned, in Chapter 8, whether extraembryonic endoderm-derived cells can support human cardiomyocyte differ entiation in vitro. Finally, we studied the TGFbet a signaling pathway, including its co-receptor Endoglin, in regulating EPDC behavior in Chapter 9 and 10.LUMC / Geneeskund

The building blocks for cardiac repair : isolation and differentiation of progenitor cells from the human heart

Unfortunately cardiovascular disease is very common, which makes it important to gain a better understanding of how we can restore the 'broken' heart after injury . Progenitor cells can serve as the 'building blocks ' for cardiac repair. Cardiac progenitor cells, which are the focus of t his thesis, can be derived from either the myocardial (CPCs) or epicardial (EPDCs) layer of the heart. Based on the idea that we want to isolate CPCs and EPDCs from the human heart and eventually use these cells for cardiac regeneration, in this thesis the f ollowing questions were investigated: • Which marker scan recognize CPCs within human cardiac tissue and how can we efficiently isolate EPDCs? ( Pa r t 1) • Which factors play a role in CPC and EPDC behavior? (Part 2) In Chapter 3, 4 and 5 novel antibodies for the isolation of CPCs are introduced and a new method to isolate and expand EPDCs is presented in Chapter 6. We studied human CPC to cardiomyocyte differentiation in Chapter 7 and questioned, in Chapter 8, whether extraembryonic endoderm-derived cells can support human cardiomyocyte differ entiation in vitro. Finally, we studied the TGFbet a signaling pathway, including its co-receptor Endoglin, in regulating EPDC behavior in Chapter 9 and 10.</p

The building blocks for cardiac repair : isolation and differentiation of progenitor cells from the human heart

Unfortunately cardiovascular disease is very common, which makes it important to gain a better understanding of how we can restore the 'broken' heart after injury . Progenitor cells can serve as the 'building blocks ' for cardiac repair. Cardiac progenitor cells, which are the focus of t his thesis, can be derived from either the myocardial (CPCs) or epicardial (EPDCs) layer of the heart. Based on the idea that we want to isolate CPCs and EPDCs from the human heart and eventually use these cells for cardiac regeneration, in this thesis the f ollowing questions were investigated: • Which marker scan recognize CPCs within human cardiac tissue and how can we efficiently isolate EPDCs? ( Pa r t 1) • Which factors play a role in CPC and EPDC behavior? (Part 2) In Chapter 3, 4 and 5 novel antibodies for the isolation of CPCs are introduced and a new method to isolate and expand EPDCs is presented in Chapter 6. We studied human CPC to cardiomyocyte differentiation in Chapter 7 and questioned, in Chapter 8, whether extraembryonic endoderm-derived cells can support human cardiomyocyte differ entiation in vitro. Finally, we studied the TGFbet a signaling pathway, including its co-receptor Endoglin, in regulating EPDC behavior in Chapter 9 and 10.</p

Cardiac Regeneration: Stem Cells and Beyond

Signal transduction in aging related disease

TGF beta and BMP signaling in cardiac cushion formation: Lessons from mice and chicken

Cardiac cushion formation is crucial for both valvular and septal development. Disruption in this process can lead to valvular and septal malformations, which constitute the largest part of congenital heart defects. One of the signaling pathways that is important for cushion formation is the TGFβ superfamily. The involvement of TGFβ and BMP signaling pathways in cardiac cushion formation has been intensively studied using chicken in vitro explant assays and in genetically modified mice. In this review, we will summarize and discuss the role of TGFβ and BMP signaling components in cardiac cushion formation

The Isolation and Culture of Primary Epicardial Cells Derived from Human Adult and Fetal Heart Specimens

Therapeutic cell differentiatio

mAb C19 targets a novel surface marker for the isolation of human cardiac progenitor cells from human heart tissue and differentiated hESCs

Therapeutic cell differentiatio

Extraembryonic Endoderm cells as a model of endoderm development

Signal transduction in aging related disease

Glycosylated Cell Surface Markers for the Isolation of Human Cardiac Progenitors

Therapeutic cell differentiatio

Human fetal and adult epicardial-derived cells: a novel model to study their activation

Cardiolog