Expression conditions used for proteins expression.

<p>Expression conditions used for proteins expression.</p

Effect of detergents on the activity of the HRV 3C protease activity.

<p>Effect of detergents on the activity of the HRV 3C protease activity.</p

Analysis of activities of the HRV 3C and TEV proteases at 25°C towards their target proteins (His8-MBP-HRV 3C-MTD or His8-MBP-TEV-MTD, His₈-MBP-HRV 3C-betaC1 or His₈-MBP-TEV-beta C1 and His₈-HRV 3C-100K or His₈-TEV-100K).

<p>Respective cleavable fusion proteins were incubated with HRV 3C protease or TEV protease at ratio of 50:1 for 1 hour and analysed by SDS-PAGE. ‘‘U” and ‘‘T” represent the cleavable fusion protein untreated and treated, respectively, with the HRV 3C or TEV protease, Note: in case of cleavage of 100K fusion protein, 2kDa band was not observed because of low %age of polyacrylamide used in SDS-PAGE.</p

List of oligonucleotides used for cloning.

<p>List of oligonucleotides used for cloning.</p

SDS-PAGE showing the HRV 3C protease activity in the presence various buffers, increasing concentrations of variety of salts, additives and detergents commonly used during extraction and purification of proteins.

<p>‘‘C” represents the cleavable fusion protein treated with protease in the absence of any additive.</p