Individual multilocus genotypes using microsatellite polymorphisms to permit the analysis of the genetic variability within and between Italian beef cattle breeds

We investigated the genetic variability within and between cattle breeds. The polymorphisms of 17 microsatellites were studied in 220 unrelated animals belonging to four Italian beef cattle breeds (Chianina, Marchigiana, Romagnola, and Piemontese). Variations of allelic frequencies were examined to characterize the breeds and their relationships. Wahlund coefficients, Polymorphism Information Content values, and Haldane exact test for Hardy-Weinberg proportions were calculated. The results show that the Hardy-Weinberg equilibrium is not always maintained. Moreover, in addition to the classical genetic distances, a new method, based on the consideration of a multilocus genotype of each animal, was set up to measure the genetic similarity between animals or within groups of animals. All the results showed that, whereas Chianina occupies an intermediate position and Piemontese is the most distinct of all four breeds, Marchigiana and Romagnola display the strongest similarity. The new method also provides evidence that average similarities are always higher within breeds than between breeds. By comparing pairwise the multilocus genotypes, it was also possible to discriminate the individuals with higher or lower genetic similarities so that each breed could be subdivided into two groups of animals in relation to their similarity to the average breed multilocus genotype. High similarities between breeds were detected, somewhat surprisingly, when the most homogeneous groups of each breed were compared. The microsatellite multilocus genotype is particularly efficient in evaluating the between- and within-breeds genetic similarities and for subgrouping genetically more homogeneous animals

From waste to resources: transforming olive leaves to hard carbon as sustainable and versatile electrode material for Li/Na-ion batteries and supercapacitors

Over the last few years, biomass-derived hard carbon materials are drawing more and more attention because of their high abundance, cost breakdown, high performance, and fast regeneration. In this context, the synthesis of hard carbon from olive leaves, a widely available by-product of table olive and olive oil industries, is here reported and its performance, as a sustainable electrode material for Li-ion batteries (LIBs), Na-ion batteries (NIBs), and supercapacitors (SCs), are evaluated. According to the in- formation acquired by structural characterization, a disordered structure is confirmed for the synthesized hard carbon. When tested as anode for LIBs and NIBs, electrodes based on Na-CMC green binder show discharge capacities of 331.0 mAh/g and 265.4 mAh/g at 1C (with minor irreversibility), respectively, with promising cycling stability. In SC application, the electrode delivers a high specific capacitance of 169.6 F/ g at 0.5 A/g and remarkable capacity retention of 96.7% after more than 20,000 cycles at 10 A/g. As a result, this work confirms the possibility to use olive leaves-derived hard carbon material for the low- cost, environmental-friendly fabrication of electrodes with high energy and power capabilities