Report on a visceral and cutaneous leishmaniases focus in the town of Jequié, State of Bahia, Brazil.

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-10-30T20:14:26Z No. of bitstreams: 1 A.J.Oliveira dos Santos Report on a visceral....pdf: 126541 bytes, checksum: 38044be3cdc8a50c28299f90c1dae838 (MD5)Made available in DSpace on 2012-10-30T20:14:26Z (GMT). No. of bitstreams: 1 A.J.Oliveira dos Santos Report on a visceral....pdf: 126541 bytes, checksum: 38044be3cdc8a50c28299f90c1dae838 (MD5) Previous issue date: 1993Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilSecretaria de Saúde do Estado da Bahia. Jequié, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasi

Dissociation between vasodilation and Leishmania infection-enhancing effects of sand fly saliva and maxadilan

In this study, the ability of maxadilan and Lutzomyia longipalpis salivary gland lysate to enhance the infection of CBA mice by Leishmania major and of BALB/c mice by L. braziliensis was tested. No difference was observed between sizes of lesion in CBA mice infected with L. major and treated or not with salivary gland lysate or maxadilan, although they were injected in concentrations that induced cutaneous vasodilation. Although parasites were more frequently observed in foot pads and spleens of animals treated with maxadilan than in the animals treated with salivary gland lysate or saline, the differences were small and not statistically significant. The lesions in BALB/c mice infected with L. braziliensis and treated with maxadilan were slightly larger than in animals that received Leishmania alone. Such differences disappeared 14 weeks after infection, and were statistically significant only in one of two experiments

Skin reactions to thimerosal and Leishmania in dogs from a leishmaniasis endemic area: it is better to keep them apart

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2012-09-19T18:02:35Z No. of bitstreams: 1 Paranhos-Silva M Skin reactions to thimerosal ....pdf: 39559 bytes, checksum: b4a024360585e65fe8c15bb1836af99d (MD5)Made available in DSpace on 2012-09-19T18:02:35Z (GMT). No. of bitstreams: 1 Paranhos-Silva M Skin reactions to thimerosal ....pdf: 39559 bytes, checksum: b4a024360585e65fe8c15bb1836af99d (MD5) Previous issue date: 2001Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCentro de Referência em Doenças Endêmicas Pirajá da Silva. Jequié, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilPositive Montenegro's skin test is a delayed type hypersensitivity reaction widely used as indicative of previous infection with Leishmania in both humans and dogs. Montenegro's antigen consists of a crude Leishmania antigen solution, usually containing thimerosal as preserving agent. In this work it is shown that a large proportion of dogs (11 out of 56) examined in an endemic area of leishmaniasis presented induration at the site of injection of a diluent containing thimerosal alone. This clearly demonstrates that thimerosal leads to a high number of false positive skin reactions in dogs and that its use in Montenegro's skin test antigenic preparations should be avoide

Development of eosinophilia in dogs intradermically inoculated with sand fly saliva and Leishmania (Leishmania) Chagasi stationary-phase promastigotes

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2014-05-28T18:47:42Z No. of bitstreams: 1 Paranhos M Development of eosinophilia in dogs....pdf: 155032 bytes, checksum: 77c4f646697c16171b3ed101d11fc7fb (MD5)Made available in DSpace on 2014-05-28T18:47:42Z (GMT). No. of bitstreams: 1 Paranhos M Development of eosinophilia in dogs....pdf: 155032 bytes, checksum: 77c4f646697c16171b3ed101d11fc7fb (MD5) Previous issue date: 1993Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz.Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório de Protozoologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Laboratório Avançado de Saúde Pública. Salvador, BA, BrasilSalivary gland lysates of the sand fly Lutzomia longipalpis have been shown to enhance the infectivity of Leishmania in mice. As shown herein, the simultaneous inoculation of Leishmania chagasi stationary-phase promastigotes and L. longipalpis salivary gland lysate by the intradermal route in a group of mongrel dogs induced a statistically significant eosinophilia, in relation to dogs inoculated with Leishmania or with salivary gland lysate only. These dogs had no evidence of infection, in spite of the high infectivity of the promastigotes when inoculated by the intravenous route

Efficacy of parasitological methods for the diagnosis of Strongyloides stercoralis and hookworm in faecal specimens

Santos, Fred Luciano Neves. Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil. “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2019-05-03T19:10:02Z No. of bitstreams: 1 Inês E J et al. - 2011 - Efficacy of parasitological methods for.pdf: 304686 bytes, checksum: f6be261c882612dbc3b47db9659ee0bc (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2019-05-06T17:00:31Z (GMT) No. of bitstreams: 1 Inês E J et al. - 2011 - Efficacy of parasitological methods for.pdf: 304686 bytes, checksum: f6be261c882612dbc3b47db9659ee0bc (MD5)Made available in DSpace on 2019-05-06T17:00:31Z (GMT). No. of bitstreams: 1 Inês E J et al. - 2011 - Efficacy of parasitological methods for.pdf: 304686 bytes, checksum: f6be261c882612dbc3b47db9659ee0bc (MD5) Previous issue date: 2011Ministério da Saúde/Fundac¸ ão de Amparo à Pesquisa do Estado da Bahia (PPSUS/FAPESB) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Brazilian agencies.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, Brasil.To compare the efficacy of stool examination for the detection of Strongyloides stercoralis and hookworm, a total of 634 stool samples from the routine laboratory service of the Pharmacia Faculty, Federal University of Bahia, Brazil, were examined by agar plate culture (APC), Baermann-Moraes and spontaneous sedimentation. The sensitivity of agar plate culture, calculated by combining results of all 3 methods, was 95% for S. stercoralis and 77.6% for hookwoorm. Moreover, APC had superior accuracy than Baermann-Moraes and spontaneous sedimentation for S. stercoralis and hookworm diagnosis, respectively. The S. stercoralis and hookworm positive samples from the laboratory routine, obtained after the previous analysis, along with those initially selected, were used to evaluate the concordance between microscopic examination and both the type of furrows left by larvae and the time for culture positivity using the APC method. Of 115 stool samples positive for S. stercoralis and 92 positive for hookworm, 110 (95.7%) and 89 (96.7%), respectively, had concordant results for furrows and morphological characteristics. The cumulative percentage of positivity increased to 94% by the third day of observation; at this time, only 19.6% of hookworm-positive samples had positive culture plates. Analyses of 74 S. stercoralis-positive stool samples stored at 4°C for 24, 48 and 72h showed the presence of larvae in 48.6%, 28.4% and 23% of samples, respectively when re-examined by the APC. As a definitive diagnosis of strongyloidiasis depends on the microscopic demonstration of parasites, increasing the sensitivity of the detection requires the use of different parasitological methods, including APC

A cross-sectional serodiagnostic survey of canine Leishmaniasis due to Leishmania Chagasi

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-07-05T18:21:09Z No. of bitstreams: 1 SILVA MP A Cross-Sectional Serodiagnostic Survey ....pdf: 1417406 bytes, checksum: 93c4d6a58dd63d552645d1d730af350d (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-07-05T18:36:21Z (GMT) No. of bitstreams: 1 SILVA MP A Cross-Sectional Serodiagnostic Survey ....pdf: 1417406 bytes, checksum: 93c4d6a58dd63d552645d1d730af350d (MD5)Made available in DSpace on 2018-07-05T18:36:21Z (GMT). No. of bitstreams: 1 SILVA MP A Cross-Sectional Serodiagnostic Survey ....pdf: 1417406 bytes, checksum: 93c4d6a58dd63d552645d1d730af350d (MD5) Previous issue date: 1996Brazilian Research Council (CNPq) and the Oswaldo Cruz Foundation (PAPES/FIOCRUZ). Antonio J. Oliveira-dos-Santos and Washington C. Santos received fellowships from the CNPq.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Instituto Osvaldo Cruz. Departamento de Imunologia. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Innsbruck. Institute for General and Experimental Pathology. Medical School. Innsbruck, AustriaJequie, a community of about 144,500 inhabitants located in the State of Bahia, Brazil, is endemic for both visceral and cutaneous leishmaniases. In the present epidemiologic study, the urban and inhabited periurban areas of the town were divided into 140 clusters of0.25 km2 each. The seroprevalence of canine Leishmania antibodies was investigated using an enzyme-linked immunosorbent assay as a screening test since its sensitivity was significantly higher than that of an indirect immunofluorescence assay. A total of 1,681 dogs was surveyed in 34 randomly sampled clusters. The overall prevalence of Leishmania antibodies in the dog population was 23.5%, with intracluster prevalences ranging from 0% to 67%. There was no correlation of these seroprevalences with the intracluster densities of canine populations, or with the distances from individual clusters to the town center. Moreover, the Leishmania transmission did not seem to follow any clear-cut spatial pattern, since large disparities in the seroprevalences of contiguous clusters were Curiously, human cases of visceral leishmaniasis have never been observed in some clusters with a relatively high prevalence of canine seroprevalences. Eight parasite isolates from seropositive dogs were found to belong to the same serodeme and zymodeme as Leishmania (L.) chagasi. The implications of these findings with respect to the epidemiology and control of American visceral leishmaniasis are discusse

Differences in the detection of Cryptosporidium and Isospora (Cystoisospora) oocysts according to the fecal concentration or staining method used in a clinical laboratory

Oliveira, Ricardo Riccio “Documento produzido em parceria ou por autor vinculado à Fiocruz, mas não consta à informação no documento”.Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2017-05-26T17:21:37Z No. of bitstreams: 1 Pacheco FT Differences in the detection of.....pdf: 662745 bytes, checksum: 89254ea4e8f283b62a084cdbea6f9304 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2017-05-26T17:46:41Z (GMT) No. of bitstreams: 1 Pacheco FT Differences in the detection of.....pdf: 662745 bytes, checksum: 89254ea4e8f283b62a084cdbea6f9304 (MD5)Made available in DSpace on 2017-05-26T17:46:41Z (GMT). No. of bitstreams: 1 Pacheco FT Differences in the detection of.....pdf: 662745 bytes, checksum: 89254ea4e8f283b62a084cdbea6f9304 (MD5) Previous issue date: 2013Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq/MCT), and Universidade Federal da Bahia (UFBA), Brazil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, Brasil / Laboratório Central de Saúde Pública Professor Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Instituto de Ciências da Saúde. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Salvador, BA, BrasilDespite the availability of many parasitological methods for detection of Cryptosporidium and Isospora (Cystoisospora) belli in fecal samples, there are uncertainties about the accuracy of these techniques in laboratory practice. In this study, 27 formalin-fixed positive stool samples for Cryptosporidium and 15 for I. belli were analyzed by 2 concentration methods, sedimentation by centrifugation (SC) and formalin-ethyl acetate (FE), and by 3 tintorial techniques, modified Ziehl-Neelsen (ZN), safranin (SF), and auramine (AR). No significant differences were observed on Cryptosporidium identification between concentration methods, while a significantly higher number of I. belli oocysts (P < 0.0001) was detected in fecal smears concentrated by the SC than by the FE method. Fecal samples processed by FE produced a median oocyst loss to the fatty ring of 34.8% for Cryptosporidium and 45.4% for I. belli. However, FE concentration provided 63% of Cryptosporidium and 100% of I. belli slides classified as superior for microscopic examination. Regarding the efficiency of staining methods, a more significant detection of Cryptosporidium oocysts was observed in fecal smears stained by ZN (P < 0.01) or AR (P < 0.05) than by the SF method. Regular to high-quality slides for microscopic examination were mostly observed in fecal smears stained with AR or ZN for Cryptosporidium and with SF or ZN for I. belli. This study suggests a great variability in oocyst power detection by routine parasitological methods, and that the most frequent intestinal coccidians in humans have specific requirements for concentration and staining