Reflected Light vs. Transmitted Light: Do They Give Different Impressions to Users?

[The 29th International Display Workshops(IDW’22)]Organizing: The Society for Information Display(SID), Date; 14-16 December 2022, Location: Fukuoka International Congress Center[第29回ディスプレイ国際ワークショップ(IDW’22)]主催: 映像情報メディア学会(ITE), 開催日: 2022年12月14日-16日, 会場: 福岡国際会議場The difference in information display between using a projector (reflected light) and a display (transmitted light) is an essential issue. Marshal McLuhan proposed in his book "Laws of Media" the well-known hypothesis that "reflected light makes people analytical and transmitted light makes them emotional." In this study, we matched the display conditions of the projector and display as much as possible. Then we presented the subjects with emotional content (landscape video) and analytical content (threedigit multiplication) and asked them to evaluate their experiences. As a result, we found no statistical difference between the projector and the display evaluations

CRISPR/Cas9-mediated rapid generation of multiple mouse lines identified Ccdc63 as essential for spermiogenesis

Spermatozoa are flagellated cells whose role in fertilization is dependent on their ability to move towards an oocyte. The structure of the sperm flagella is highly conserved across species, and much of what is known about this structure is derived from studies utilizing animal models. One group of proteins essential for the movement of the flagella are the dyneins. Using the advanced technology of CRISPR/Cas9 we have targeted three dynein group members; Dnaic1, Wdr63 and Ccdc63 in mice. All three of these genes are expressed strongly in the testis. We generated mice with amino acid substitutions in Dnaic1 to analyze two specific phosphorylation events at S124 and S127, and generated simple knockouts of Wdr63 and Ccdc63. We found that the targeted phosphorylation sites in Dnaic1 were not essential for male fertility. Similarly, Wdr63 was not essential for male fertility; however, Ccdc63 removal resulted in sterile male mice due to shortened flagella. This study demonstrates the versatility of the CRISPR/Cas9 system to generate animal models of a highly complex system by introducing point mutations and simple knockouts in a fast and efficient manner.Young, S.A.M.; Miyata, H.; Satouh, Y.; Kato, H.; Nozawa, K.; Isotani, A.; Aitken, R.J.; Baker, M.A.; Ikawa, M. CRISPR/Cas9-Mediated Rapid Generation of Multiple Mouse Lines Identified Ccdc63 as Essential for Spermiogenesis. Int. J. Mol. Sci. 2015, 16, 24732-24750. https://doi.org/10.3390/ijms16102473

The MAXI Mission on the ISS: Science and Instruments for Monitoring All Sky X-Ray Images

The MAXI (Monitor of All-sky X-ray Image) mission is the first astronomical payload to be installed on the Japanese Experiment Module-Exposed Facility (JEM-EF) on the ISS. It is scheduled for launch in the middle of 2009 to monitor all-sky X-ray objects on every ISS orbit. MAXI will be more powerful than any previous X-ray All Sky Monitor (ASM) payloads, being able to monitor hundreds of AGN. MAXI will provide all sky images of X-ray sources of about 20 mCrab in the energy band of 2-30 keV from observation on one ISS orbit (90 min), about 4.5 mCrab for one day, and about 1 mCrab for one month. A final detectability of MAXI could be 0.2 mCrab for 2 year observations.Comment: 12 pages, 11 figures, accepted for publication in Publications of the Astronomical Society of Japa

Degradation of Mutant Protein Aggregates within the Endoplasmic Reticulum of Vasopressin Neurons

Misfolded or unfolded proteins in the ER are said to be degraded only after translocation or isolation from the ER. Here, we describe a mechanism by which mutant proteins are degraded within the ER. Aggregates of mutant arginine vasopressin (AVP) precursor were confined to ER-associated compartments (ERACs) connected to the ER in AVP neurons of a mouse model of familial neurohypophysial diabetes insipidus. The ERACs were enclosed by membranes, an ER chaperone and marker protein of phagophores and autophagosomes were expressed around the aggregates, and lysosomes fused with the ERACs. Moreover, lysosome-related molecules were present within the ERACs, and aggregate degradation within the ERACs was dependent on autophagic-lysosomal activity. Thus, we demonstrate that protein aggregates can be degraded by autophagic-lysosomal machinery within specialized compartments of the ER

Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis

Keisuke SHIMADA, Hirotaka KATO, Haruhiko MIYATA, Masahito IKAWA, Glycerol kinase 2 is essential for proper arrangement of crescent-like mitochondria to form the mitochondrial sheath during mouse spermatogenesis, Journal of Reproduction and Development, 2019, Volume 65, Issue 2, Pages 155-162, Released April 12, 2019, [Advance publication] Released January 21, 2019, Online ISSN 1348-4400, Print ISSN 0916-8818, https://doi.org/10.1262/jrd.2018-136, https://www.jstage.jst.go.jp/article/jrd/65/2/65_2018-136/_article/-char/e