A Study of Long-Term Metabolism of Nucleic Acid in the Repair Process of Cancer Cells with Cis-diamminedichloroplatinum (II)

CDDP is one of the anticancer drugs in which the presence of the repair mechanism has been pointed out. Thus, using established pulmonary cancer cells, we evaluated the metabolism of DNA and RNA in damaged cells treated with CDDP (0.1, 1.0, 10μg/ml) by means of 3 H-TdR . 3 H-UR incorporation assay for 15 days referring to the growth curve and colony forming assay. 3H-TdR uptake inhibition rate in CDDP treatment was higher than 3H-UR uptake inhibition rate and showed good correlation with the inhibition rate of colony formation. In order to evaluate the activity of metabolism of DNA and RNA, the experimental period was divided into two parts : an early stage (2-10 days) and a later stage (7-15 days), and changing phases of uptake of 3H-TdR and 3H-UR was estimated as an increased rate. In the early stage, cells treated CDDP at lower concentrations tended to show more active DNA and RNA metabolism, especially RNA. In the later stage, cells with 0.1μg/ml CDDP which had shown only slight growth inhibition in the early stage revealed lower activity than that in the early stage. Cells with 1.0vg/ml CDDP which showed regrowth after 7 days, and cells with 10μg/ml CDDP which were not recognized with proliferation were observed with more active metabolism of RNA than that in the early stage. Those results indicated that metabolism of RNA is closely associated with the cellular repair process by CDDP

A Trial in Estimating Chemosensitivity from Biopsy Specimen by Flow Cytometry

To select the sensitive anticancer agents for the treatment of patients with carcinoma, chemosensitivity should be timely assessed from biopsy specimen for patients with gastric carcinoma. Histogram of DNA analysis from biopsy specimens revealed the disappearance of GZM accumulation in reflection of effects of anticancer drugs prior to the appearance of clinical signs. In conclusion, DNA analysis from biopsy specimens is of great value in assessing the efficacy of anticancer drugs in the course of drug treatment and also in knowing the drug sensitivity to the tumor timely

Survey of Period Variations of Superhumps in SU UMa-Type Dwarf Novae. II: The Second Year (2009-2010)

As an extension of the project in Kato et al. (2009, arXiv:0905.1757), we collected times of superhump maxima for 61 SU UMa-type dwarf novae mainly observed during the 2009-2010 season. The newly obtained data confirmed the basic findings reported in Kato et al. (2009): the presence of stages A-C, as well as the predominance of positive period derivatives during stage B in systems with superhump periods shorter than 0.07 d. There was a systematic difference in period derivatives for systems with superhump periods longer than 0.075 d between this study and Kato et al. (2009). We suggest that this difference is possibly caused by the relative lack of frequently outbursting SU UMa-type dwarf novae in this period regime in the present study. We recorded a strong beat phenomenon during the 2009 superoutburst of IY UMa. The close correlation between the beat period and superhump period suggests that the changing angular velocity of the apsidal motion of the elliptical disk is responsible for the variation of superhump periods. We also described three new WZ Sge-type objects with established early superhumps and one with likely early superhumps. We also suggest that two systems, VX For and EL UMa, are WZ Sge-type dwarf novae with multiple rebrightenings. The O-C variation in OT J213806.6+261957 suggests that the frequent absence of rebrightenings in very short-Porb objects can be a result of sustained superoutburst plateau at the epoch when usual SU UMa-type dwarf novae return to quiescence preceding a rebrightening. We also present a formulation for a variety of Bayesian extension to traditional period analyses.Comment: 63 pages, 77 figures, 1 appendix, Accepted for publication in PASJ, data correctio

Perforated Peptic Ulcer of the Jejunum with Ectopic Gastric Mucosa

Ectopic gastric mucosa (EGM) of the jejunum is rare. We report an 81-year-old man with perforated peptic ulcer of the jejunum originating from EGM. Emergency segmental resection of the jejunum was performed with satisfactory immediate results. Diagnosis after surgery was established by histopathology. In addition, sections were examined immunohistochemically using antibodies against MUC5AC, MUC6 and MUC2. Hematoxylin-eosin-stained sections showed glandular differentiated tissue interposed between normal small bowel epithelium. EGM was positive for MUC5AC and MUC6, and negative for MUC2. Histological examination of the resected specimen confirmed that the perforation was caused by a peptic jejunal ulcer associated with EGM. To our knowledge, this is the first reported case of perforated peptic ulcer of the jejunum with EGM in an elderly patient