Total RNA samples prepared from 10 different tissues of 4-week-old hEPO/ΔRS and control ΔRS chimaeras were subjected to RT–PCR analysis using hEPO (35 cycles) and Cκ (35 cycles) specific primer pairs

<p><b>Copyright information:</b></p><p>Taken from "A novel transgenic chimaeric mouse system for the rapid functional evaluation of genes encoding secreted proteins"</p><p>Nucleic Acids Research 2005;33(9):e85-e85.</p><p>Published online 24 May 2005</p><p>PMCID:PMC1140086.</p><p>© The Author 2005. Published by Oxford University Press. All rights reserved</p> The sizes of resulting PCR products in all experiments were consistent with the expected sizes predicted from reported cDNA sequences. Murine GAPDH (25 cycles) was used as a control