The Impact of Whole Sesame Seeds on the Expression of Key-Genes Involved in the Innate Immunity of Dairy Goats

Whole sesame seeds (WSS) are rich in both linoleic acid (LA) and lignans. However, their impact on the innate immunity of goats is not well studied. Twenty-four goats were divided into three homogeneous sub-groups; comprise one control (CON) and two treated (WWS5 and WWS10). In the treated groups, WSS were incorporated in the concentrates of the CON at 5 (WSS5) and 10% (WSS10) respectively, by partial substitution of both soybean meal and corn grain. The expression levels of MAPK1, IL6, TRIF, IFNG, TRAF3, and JUND genes in the neutrophils of WSS10 fed goats were reduced significantly compared with the CON. The same was found for the expression levels of IFNG and TRAF3 genes in the neutrophils of WSS5 fed goats. Both treated groups primarily affected the MYD88-independent pathway. The dietary supplementation of goats with WSS might be a good nutritional strategy to improve their innate immunity

The effect of whole sesame seeds on milk chemical composition, fatty acid profile and antioxidant status in goats

Whole sesame seeds (WSS), although rich in linoleic acid, have not been studied for their impact on the milk fatty acid (FA) profile. Twenty-four goats were divided into three homogeneous subgroups. A control (CON) group and treated groups, in which WSS were incorporated in the concentrates of the CON at 5% (WSS5) and 10% (WSS10), respectively, by partial substitution of both soybean meal and corn grain, were evaluated. Significant increases in the milk fat and total solids contents of goats fed the WSS10 diet compared with the CON were found. In the milk of WSS10-fed goats, the proportions of long-chain fatty acids, monounsaturated fatty acids and unsaturated fatty acids (UFAs) were increased, while those of medium-chain fatty acids and saturated fatty acids (SFAs) were decreased compared with those of the CON group. Significant decreases in the proportions of short-chain fatty acids, in the SFA/UFA ratio and in the atherogenicity index value in milk of treated goats were observed. The proportions of C15:0 and C17:0 FAs in the blood plasma of WSS10-fed animals compared with CON-fed animals were reduced significantly. Superoxide dismutase and catalase activities increased significantly in the blood plasma of WSS10-fed goats compared with CON-fed goats. Additionally, in the treated goats, the protein carbonyls (PC) in blood plasma and both PC and malondialdehyde contents in milk declined significantly. A significant enhancement in the total antioxidant capacity [measured by the ferric reducing ability of plasma (FRAP)] in the milk of WSS10-fed goats compared with the CON was found. Similarly, an increase in the FRAP value of milk of WSS5-fed goats compared with CON-fed goats was observed. In conclusion, the highest inclusion level of WSS (WSS10) in goat diets improves the FA profile and the oxidative stability of milk and improves the organism's antioxidant status

The Impact of Whole Sesame Seeds on the Expression of Key-Genes Involved in the Innate Immunity of Dairy Goats

Whole sesame seeds (WSS) are rich in both linoleic acid (LA) and lignans. However, their impact on the innate immunity of goats is not well studied. Twenty-four goats were divided into three homogeneous sub-groups; comprise one control (CON) and two treated (WWS5 and WWS10). In the treated groups, WSS were incorporated in the concentrates of the CON at 5 (WSS5) and 10% (WSS10) respectively, by partial substitution of both soybean meal and corn grain. The expression levels of MAPK1, IL6, TRIF, IFNG, TRAF3, and JUND genes in the neutrophils of WSS10 fed goats were reduced significantly compared with the CON. The same was found for the expression levels of IFNG and TRAF3 genes in the neutrophils of WSS5 fed goats. Both treated groups primarily affected the MYD88-independent pathway. The dietary supplementation of goats with WSS might be a good nutritional strategy to improve their innate immunity

Feeding level regulates the expression of some genes involved with programed cell death and remodeling in goat and sheep mammary tissue

Mammary tissue (MT) turnover is characterized by programed cell death and remodeling which might be affected by both feeding level and animal species. Thus, twenty-four dairy goats and the same number of sheep were assigned to three homogenous sub-groups per animal species and fed the same diet in quantities which met 70% (FL70), 100% (FL100) and 130% (FL130) of their daily energy and crude protein requirements. Individual MT samples were taken by biopsy from the animals on the 30th and 60th experimental day. The results showed, in the first sampling time, a significant reduction in the mRNA abundance for selected genes involved in programed cell death in both FL 70 fed goats (STAT3 and BECN1) and sheep (CASPASE8 and BECN1) compared with the respective FL100 groups. The FL130, in comparison with the FL100, caused a significant increase in transcripts accumulation of STAT3 gene in both sampling times and CASPASE8 gene in the second sampling time in goat MT, while the opposite happened for the mRNA expression of CASPASE8 and BECN1 genes in sheep MT, but only in the first sampling time. Moreover, a significant up regulation in the mRNA levels of MMP2 gene in MT of FL130 fed sheep was observed. The FL130, in comparison with the FL70, caused an enhancement in the mRNA expression levels of BECN1, CASPASE8, BAX and STAT3 genes in goat MT only. It was also shown that apoptosis and autophagy can be affected simultaneously by the feeding level. Overfeeding affects MT programed cell death and remodeling by a completely different way in goats than sheep. In conclusion, feeding level and animal species have strong effects on both MT programed cell death (apoptosis and autophagy) and remodeling but the molecular mechanisms need further investigation

Influence of dietary sesame meal, vitamin E and selenium supplementation on milk production, composition, and fatty acid profile in dairy goats

Although sesame oil meal is rich in linoleic acid, and both selenium (Se) and vitamin E (VitE) protect prone-unsaturated fatty acids (UFA) from oxidation, their influence on goats’ milk fatty acids (FAs) profile have not been studied. Thus, thirty dairy goats were divided into five groups and fed with alfalfa hay and concentrates. Two types of concentrates were used; one for the control (CON) and another for the treated groups. In the concentrate of the treated groups which contained sesame oil meal, apart from the quantities of VitE and Se included in the trace mineral-vitamin premix, either no extra VitE and Se (SOM) or extra 60 mg of VitE/Kg of concentrate (SOME), or 0.1 mg organic Se/Kg of concentrate (SOMSe) or its combination (60 mg of VitE and 0.1 mg organic Se/Kg of concentrate) (SOMESe) were incorporated. In blood plasma, the proportion of C14:0 reduced significantly in the SOME-fed goats compared with CON-fed goats while that of C16:0 reduced significantly in those goats fed the SOM, SOMSe, SOMESe diets. The proportions of medium-chain FAs (MCFA) and saturated FAs (SFA), and the atherogenicity index value reduced significantly in the milk of treated animals. Significant was also the decline in the SFA/UFA ratio in the milk of goats fed with the SOM, SOME and SOMESe diets. On the contrary, the proportions of long-chain FAs in the milk of SOM and SOMESe fed goats increased significantly. A significant increase in the proportions of UFA in the milk of SOME and SOMESe-fed goats and in the proportions of MUFA in those goats fed the SOM, SOME and SOMESe diets was found. In conclusion, the SOMESe diet can be considered as the most effective nutritional strategy to affect positively the goats’ milk FAs profile since the sharpest modifications in the aforementioned FAs were observed with this dietary treatment

Cocoa husks fed to lactating dairy ewes affect milk fatty acid profile and oxidative status of blood and milk

The objective of this study was to evaluate the effect of cocoa husks (CH) containing theobromine on milk fatty acids (FA) and on milk and blood oxidative status in dairy ewes. The experiment was carried out with 24 dairy ewes and it lasted 8 weeks with 3 weeks of adaptation period and 5 weeks of experimental period. Animals were divided in three homogeneous groups and all ewes were fed with the same basal diet, composed of a total mix ration (TMR). The first group (CON) was fed with the TMR and a supplementation of 100 g/d per head of soybean hulls, while the second (CH50) and the third groups (CH100) were fed with the TMR and a supplementation of 50 and 100 g/d per head of CH, respectively. Blood samples were analyzed for the activities of superoxide dismutase (SOD), glutathione transferase (GSTS), glutathione reductase (GR) and glutathione peroxidase (GPx), while milk samples were analyzed for SOD, GR and lactoperoxidase (LPO). Total antioxidant capacity and oxidative stress biomarkers in blood and milk samples were determined. Orthogonal polynomial contrasts (linear and quadratic) were used to investigate the effect of the diet on the variables. The C16:0 and MCFA (medium-chain fatty acids) decreased, whereas C18:0 and LCFA (long-chain fatty acids) increased quadratically with the dose of CH. The supplementation of CH decreased linearly the OBCFA (branched-chain fatty acid). Regarding antioxidant results, mean protein carbonyls (PC) decreased linearly and SOD activity tended to increase linearly in blood with increasing CH dose. Meanwhile, antioxidant analysis of milk samples showed that CH supplementation increased quadratically the LPO and tended to decrease quadratically the malondialdehyde level (MDA). In conclusion, the addition of CH as supplement to the diet of ewes could modify the milk FA profile and could improve antioxidant capacity of blood and milk

Effects of Supplementing Rumen-Protected Methionine and Lysine on Milk Performance and Oxidative Status of Dairy Ewes

There is limited information on the impact of dietary supplementation with separate rumen-protected (RP) amino acids (AA), or with their combination, on ewes’ oxidative status. Sixty ewes were divided into five groups; C: basal diet (control); M: basal diet + 6 g/ewe RP methionine; L: basal diet + 5 g/ewe RP lysine; LML: basal diet + 6 g methionine and 5 g lysine/ewe; and HML: basal diet + 12 g methionine + 5 g lysine/ewe. Milk’s fat content increased in RP-AA fed ewes, while that of protein in M and L only. In blood plasma, the malondialdehyde (MDA) content was reduced in the M, LML, and HML compared to C-fed ewes. An increase in glutathione transferase activity in the blood plasma of the M and LML compared to the C and HML-fed ewes were found. In milk, lower values of the ferric reducing ability of plasma (FRAP) in the LML and HML-fed ewes and of 2,2′-Azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) in LML only, were found. Lysine increased milk’s FRAP values and MDA content. Both L and HML diets increased milk’s protein carbonyls content. Methionine improves the organism’s oxidative status, without adversely affecting milk’s oxidative stability. Lysine dietary inclusion affects negatively the oxidative stability of milk

Effects of supplementing rumen-protected methionine and lysine on milk performance and oxidative status of dairy ewes

There is limited information on the impact of dietary supplementation with separate rumen-protected (RP) amino acids (AA), or with their combination, on ewes' oxidative status. Sixty ewes were divided into five groups; C: basal diet (control); M: basal diet + 6 g/ewe RP methionine; L: basal diet + 5 g/ewe RP lysine; LML: basal diet + 6 g methionine and 5 g lysine/ewe; and HML: basal diet + 12 g methionine + 5 g lysine/ewe. Milk's fat content increased in RP-AA fed ewes, while that of protein in M and L only. In blood plasma, the malondialdehyde (MDA) content was reduced in the M, LML, and HML compared to C-fed ewes. An increase in glutathione transferase activity in the blood plasma of the M and LML compared to the C and HML-fed ewes were found. In milk, lower values of the ferric reducing ability of plasma (FRAP) in the LML and HML-fed ewes and of 2,2'-Azino-bis 3-ethylbenzthiazoline-6-sulfonic acid (ABTS) in LML only, were found. Lysine increased milk's FRAP values and MDA content. Both L and HML diets increased milk's protein carbonyls content. Methionine improves the organism's oxidative status, without adversely affecting milk's oxidative stability. Lysine dietary inclusion affects negatively the oxidative stability of milk

Dietary Supplementation of a Live Yeast Product on Dairy Sheep Milk Performance, Oxidative and Immune Status in Peripartum Period

This study evaluated the dietary administration of Saccharomyces cerevisiae live yeast on milk performance and composition, oxidative status of both blood plasma and milk, and gene expression related to the immune system of lactating ewes during the peripartum period. Chios ewes were fed either a basal diet (BD) (Control, n = 51) or the BD supplemented with 2 g of a live yeast product/animal (ActiSaf, n = 53) from 6 weeks prepartum to 6 weeks postpartum. Fatty acid profile, oxidative, and immune status were assessed in eight ewes per treatment at 3 and 6 weeks postpartum. The β-hydroxybutyric acid concentration in blood of ActiSaf fed ewes was significantly lower in both pre- and postpartum periods. A numerical increase was found for the milk yield, fat 6% corrected milk (Fat corrected milk (FCM6%)), and energy corrected milk yield (ECM) in ActiSaf fed ewes, while daily milk fat production tended to increase. The proportions of C15:0, C16:1, C18:2n6t, and C18:3n3 fatty acids were increased in milk of ActiSaf fed ewes, while C18:0 was decreased. Glutathione reductase in blood plasma was increased (p = 0.004) in ActiSaf fed ewes, while total antioxidant capacity measured by 2,2′-Azino-bis (3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) method was decreased (p < 0.001). Higher ABTS values were found in the milk of the treated group. The relative transcript levels of CCL5, CXCL16, and IL8 were suppressed, while that of IL1B tended to decrease (p = 0.087) in monocytes of ActiSaf fed ewes. In conclusion, the dietary supplementation of ewes with S. cerevisiae, improved the energy utilization and tended to enhance milk performance with simultaneous suppression on mRNA levels of pro-inflammatory genes during the peripartum period

Plant Feed Additives as Natural Alternatives to the Use of Synthetic Antioxidant Vitamins in Livestock Animal Products Yield, Quality, and Oxidative Status: A Review

The interest for safe and natural foods of animal origin is currently increasing the use of plant feed additives (PFA) as antioxidants in animal nutrition. However, studies with livestock animals dealing with PFA as antioxidants are scarce. The aim of the present review was to evaluate the antioxidant impact of PFA compared with synthetic vitamins on animal food product yield and quality. For this purpose, peer-reviewed studies published between 2000 and 2020 were collected. Most papers were carried out on ruminants (n = 13), but PFA were also tested in swine (n = 6) and rabbits (n = 2). The inclusion of PFA in the diets of pigs, rabbits, and ruminants improved the products' quality (including organoleptic characteristics and fatty acids profile), oxidative stability, and shelf life, with some impacts also on their yields. The effects of PFA are diverse but often comparable to those of the synthetic antioxidant vitamin E, suggesting their potential as an alternative to this vitamin within the diet