Temporal Control of the TGF-β Signaling Network by Mouse ESC MicroRNA Targets of Different Affinities.

Although microRNAs (miRNAs) function in the control of embryonic stem cell (ESC) pluripotency, a systems-level understanding is still being developed. Through the analysis of progressive Argonaute (Ago)-miRNA depletion and rescue, including stable Ago knockout mouse ESCs, we uncover transforming growth factor beta (TGF-β) pathway activation as a direct and early response to ESC miRNA reduction. Mechanistically, we link the derepression of weaker miRNA targets, including TGF-β receptor 1 (Tgfbr1), to the sensitive TGF-β pathway activation. In contrast, stronger miRNA targets impart a more robust repression, which dampens concurrent transcriptional activation. We verify such dampened induction for TGF-β antagonist Lefty. We find that TGF-β pathway activation contributes to the G1 cell-cycle accumulation of miRNA-deficient ESCs. We propose that miRNA target affinity is a determinant of the temporal response to miRNA changes, which enables the coordination of gene network responses

The Antimicrobial Effect of Silver Ion Impregnation into Endodontic Sealer against Streptococcus mutans.

Pulpal and periradicular diseases are primarily caused by bacterial invasion of the root canal system as a result of caries progression. The presence of residual bacteria at the time of root canal completion (obturation) is associated with significantly higher rate of treatment failure. Re-infection of obturated root canals can be potentially prevented by enhancing the antibacterial activities of root canal obturation materials. We evaluated, in an in vitro model, the antimicrobial efficacy of silver ions added to a common endodontic sealer. For that purpose we performed growth inhibition studies and bacterial viability tests. We measured the zone of inhibition, optical density and performed confocal laser scanning microscopy. Our results show that the silver ions enhance the antimicrobial activity of the root canal sealer against Streptococcus mutans. This study approach may hold promise for studying other biologically based therapies and therefore increasing the success rate of routine orthograde root canal treatment

Multiwavelength Study of NGC 281 Region

We present a multiwavelength study of the NGC 281 complex which contains the young cluster IC 1590 at the center, using deep wide-field optical UBVI_c photometry, slitless spectroscopy along with archival data sets in the near-infrared (NIR) and X-ray. The extent of IC 1590 is estimated to be ~6.5 pc. The cluster region shows a relatively small amount of differential reddening. The majority of the identified young stellar objects (YSOs) are low mass PMS stars having age <1-2 Myr and mass 0.5-3.5 M_\odot. The slope (\Gamma) of the mass function for IC 1590, in the mass range 2 < M/M_\odot \le 54, is found to be -1.11+-0.15. The slope of the K-band luminosity function (0.37+-0.07) is similar to the average value (~0.4) reported for young clusters. The distribution of gas and dust obtained from the IRAS, CO and radio maps indicates clumpy structures around the central cluster. The radial distribution of the young stellar objects, their ages, \Delta(H-K) NIR-excess, and the fraction of classical T Tauri stars suggest triggered star formation at the periphery of the cluster region. However, deeper optical, NIR and MIR observations are needed to have a conclusive view of star formation scenario in the region. The properties of the Class 0/I and Class II sources detected by using the Spitzer mid-infrared observations indicate that a majority of the Class II sources are X-ray emitting stars, whereas X-ray emission is absent from the Class 0/I sources. The spatial distribution of Class 0/I and Class II sources reveals the presence of three sub-clusters in the NGC 281 West region.Comment: 29 pages, 21 figures and 11 tables, Accepted for the publication in PAS

Development of superconducting conductors for Large Helical Device

The superconducting helical coils of the Large Helical Device (LHD) require superconducting conductors with large current capacities (from 20 kA to 30 kA) and high current densities (55 A/mm2 at 8 T). An NbTi superconductor/bin with pool boiling is being used because of the large electromagnetic force and the complicated helical windings. Several conductors are designed to show how the difference of the position of pure aluminum in the conductors affects the stability and the mechanical properties. Scaled-down R&D conductors with operational currents from 7 kA to 10 kA were made on an experimental basis. The superconducting characteristics, stability, and mechanical properties of these scaled-down conductors were tested. The design and the test results concerning the superconducting characteristics are describe

Nonlinear magnetic responses at the phase boundaries around helimagnetic and skyrmion lattice phases in MnSi: Evaluation of robustness of noncollinear spin texture

The phase diagram of a cubic chiral magnet MnSi with multiple Dzyaloshinskii-Moriya (DM) vectors as a function of temperature T and dc magnetic field Hdc was investigated using intensity mapping of the odd-harmonic responses of ac magnetization (M1ω andM3ω), and the responses at phase boundaries were evaluated according to a prescription [J. Phys. Soc. Jpn. 84, 104707 (2015)]. By evaluating M3ω/M1ω appearing at phase boundaries, the robustness of noncollinear spin texture in both the helimagnetic (HM) and the skyrmion lattice (SkL) phases of MnSi was discussed. The robustness of vortices-type solitonic texture SkL in MnSi is smaller than those of both the single DM HM and chiral soliton lattice phases of a monoaxial chiral magnet Cr1/3NbS2, and furthermore the robustness of the multiple DM HM phase in MnSi is smaller than that of its SkL. Through magnetic diagnostics over the wide T -Hdc range, we found a new paramagnetic (PM) region with ac magnetic hysteresis, where spin fluctuations have been observed via electrical magnetochiral effect. The anomalies observed in the previous ultrasonic attenuation measurement correspond to the peak positions of out-of-phase M1ω. The appearance of a new PM region occurs at a characteristic magnetic field, above which indeed the SkL phase appears. It has us suppose that the new PM region could be a phase with spin fluctuation like the skyrmion gas phase