Cortisol reactivity to psychosocial stress is greater in sexual risk takers

Several studies have reported an association between deviant behaviour and cortisol reactivity to stress. However relatively few studies have investigated the relationship between psychobiological stress reactivity and sexual risk taking behaviours. In the present study, cortisol reactivity to the Trier Social Stress Test (TSST) was measured in 26 healthy young adults prior to the administration of a sexual health and behaviour questionnaire. The cortisol response to the TSST was greater in those individuals who reported that at least one of their previous two sexual partners was someone whom they had just met. Results are discussed in context of a model which suggests that early life stress dysregulates the hypothalamic-pituitary-adrenal (HPA) axis and increases the likelihood of later life risk taking behaviour. The findings have implications in terms of improving our understanding of psychobiological factors which predispose individuals to engage in adverse sexual health behaviours

Ion channels of cultured human retinal pigment epithelial cells

In this thesis, the patch clamp technique has been used to examine single channels of cultured human retinal epithelial (RPE) cells. Three main channel types were detected in the cell-attached recording mode. They had mean conductances of 24 pS, 59 pS and 96 pS with physiological solutions present. All three channel types were primarily permeable to potassium. Under physiological conditions the activity of all three channel types was voltage dependant, and increased as the patches were depolarized. However, all channel types had low levels of activity at the membrane potential. The activity of the 96 pS channel type was time dependent; the channel inactivated in response to depolarizing steps. This channel type was also sensitive to the concentration of calcium; when the cells were bathed in low calcium solution the channel open probability fell towards zero. The 96 pS channel is thus similar to the maxi-K channels in other epithelia. The presence of microvilli on the top surface of these cells supports a comparison to the apical membrane. Single channel recordings on fresh bovine RPE were also attempted, but most of the channel openings were obscured by a large, noisy conductance. The voltage-sensitivity of channel activity found in cultured cells suggests that these channels may provide a mechanism for integrating dynamic transport across the RPE

Graph-to-Graph Meaning Representation Transformations for Human-Robot Dialogue

In support of two-way human-robot communication, we leverage Abstract Meaning Representation (AMR) to capture the core semantic content of natural language search and navigation instructions. In order to effectively map AMR to a constrained robot action specification, we develop a set of in-domain, task-specific AMR graphs augmented with speech act and tense and aspect information not found in the original AMR. This paper presents our efforts and results in transforming AMR graphs into our in-domain graphs by employing both rule-based and classifier-based methods, thereby bridging the gap from entirely unconstrained natural language input to a fixed set of robot actions

Growing Pains or Opportunities? A Customer Survey of Three Farmers\u27 Markets in One Rural Community

The continued growth of farmers\u27 markets is presenting new challenges to Extension. As the number of markets expands, how can Extension help those in the same community work together for mutual benefit? The study reported here examined similarities and differences among customers attending three different farmers\u27 markets within a single locality in Gettysburg, Pennsylvania. Based on 370 customer surveys, study results underscore the diversity of markets operating within the same community and provide insights into ways Extension might assist markets to work together to expand their shared customer base, increase revenues, and better serve local residents

Feasibility study of early outpatient review and early cardiac rehabilitation after cardiac surgery: mixed-methods research design-a study protocol.

INTRODUCTION: Following cardiac surgery, patients currently attend an outpatient review 6 weeks after hospital discharge, where recovery is assessed and suitability to commence cardiac rehabilitation (CR) is determined. CR is then started from 8 weeks. Following a median sternotomy, cardiac surgery patients are required to refrain from upper body exercises, lifting of heavy objects and other strenuous activities for 12 weeks. A delay in starting CR can prolong the recovery process, increase dependence on family/carers and can cause frustration. However, current guidelines for activity and exercise after median sternotomy have been described as restrictive, anecdotal and increasingly at odds with modern clinical guidance for CR. This study aims to examine the feasibility of bringing forward outpatient review and starting CR earlier. METHODS AND ANALYSES: This is a multicentre, randomised controlled, open feasibility trial comparing postoperative outpatient review 6 weeks after hospital discharge, followed by CR commencement from 8 weeks (control arm) versus, postoperative outpatient review 3 weeks after hospital discharge, followed by commencement of CR from 4 weeks (intervention arm). The study aims to recruit 100 eligible patients, aged 18-80 years who have undergone elective or urgent cardiac surgery involving a full median sternotomy, over a 7-month period across two centres. Feasibility will be measured by consent, recruitment, retention rates and attendance at appointments and CR sessions. Qualitative interviews with trial participants and staff will explore issues around study processes and acceptability of the intervention and the findings integrated with the feasibility trial outcomes to inform the design of a future full-scale randomised controlled trial. ETHICS AND DISSEMINATION: Ethics approval was granted by East Midlands-Derby Research Ethics Committee on 10 January 2019. The findings will be presented at relevant conferences disseminated via peer-reviewed research publications, and to relevant stakeholders. TRIAL REGISTRATION NUMBER: ISRCTN80441309

Integrative multiomics analysis highlights immune-cell regulatory mechanisms and shared genetic architecture for 14 immune-associated diseases and cancer outcomes

Developing functional insight into the causal molecular drivers of immunological disease is a critical challenge in genomic medicine. Here, we systematically apply Mendelian randomization (MR), genetic colocalization, immune-cell-type enrichment, and phenome-wide association methods to investigate the effects of genetically predicted gene expression on ten immune-associated diseases and four cancer outcomes. Using whole blood-derived estimates for regulatory variants from the eQTLGen consortium (n = 31,684), we constructed genetic risk scores for 10,104 genes. Applying the inverse-variance-weighted MR method transcriptome wide while accounting for linkage disequilibrium structure identified 664 unique genes with evidence of a genetically predicted effect on at least one disease outcome (p < 4.81 × 10(−5)). We next undertook genetic colocalization to investigate cell-type-specific effects at these loci by using gene expression data derived from 18 types of immune cells. This highlighted many cell-type-dependent effects, such as PRKCQ expression and asthma risk (posterior probability = 0.998), which was T cell specific. Phenome-wide analyses on 311 complex traits and endpoints allowed us to explore shared genetic architecture and prioritize key drivers of disease risk, such as CASP10, which provided evidence of an effect on seven cancer-related outcomes. Our atlas of results can be used to characterize known and novel loci in immune-associated disease and cancer susceptibility, both in terms of elucidating cell-type-dependent effects as well as dissecting shared disease pathways and pervasive pleiotropy. As an exemplar, we have highlighted several key findings in this study, although similar evaluations can be conducted via our interactive web platform