3 research outputs found
5. 慢性円板状エリテマトーデスに続発せる棘細胞癌の1例について(第434(B)回千葉医学会例会 第15回千葉皮膚科臨床談話会)
Additional file 3: Table S3. Details of proteins identified in the study with reports on whether or not reported in PPD and gene ontology-based classification
MOESM2 of Synovial fluid proteome in rheumatoid arthritis
Additional file 2: Table S2. List of proteins and their corresponding peptides identified in the study
Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach
Acute
ischemic stroke (AIS) is a devastating cerebrovascular disorder
that leads to permanent physical and neurological disabilities in
adults worldwide. Proteins associated with stroke pathogenesis may
appear in the serum of AIS patients due to blood–brain barrier
dysfunction, thus permitting the development of blood-based biomarkers
for early diagnosis of stroke. These biomarkers could perhaps be an
adjunct to the existing imaging modalities and aid in better management
and therapeutic intervention during the course of the disease. For
this exploratory study, a combination of multiplexed isobaric tagging
using iTRAQ reagents and high resolution tandem mass spectrometry
was used to identify differentially expressed proteins in serum samples
from AIS patients. The quantitative proteomic analysis of serum from
both AIS and control subjects revealed 389 high confidence protein
identifications and their relative levels. Among them, 60 proteins
showed a ≥1.5-fold change in the AIS subjects. We verified
the altered serum levels of candidate proteins such as vWF, ADAMTS13,
S100A7, and DLG4 through ELISA, and the results also corroborate with
the experimental findings. vWF and ADAMTS13 are key players that regulate
blood hemostasis, and their altered concentration may contribute to
endothelial dysfunction. S100A7 is a novel candidate protein identified in this
study that is also known to mediate inflammation, endothelial proliferation,
and angiogenesis. The current study provided a potential and novel
biomarker panel that may in turn provide diagnostic aid to the existing
imaging modalities for the rapid diagnosis of ischemic strok