Changes of Host Immunity Mediated by IFN-γ+ CD8+ T Cells in Children with Adenovirus Pneumonia in Different Severity of Illness

The host immunity of patients with adenovirus pneumonia in different severity of illness is unclear. This study compared the routine laboratory tests and the host immunity of human adenovirus (HAdV) patients with different severity of illness. A co-cultured cell model in vitro was established to verify the T cell response in vitro. Among 140 patients with confirmed HAdV of varying severity, the number of lymphocytes in the severe patients was significantly reduced to 1.91 × 109/L compared with the healthy control (3.92 × 109/L) and the mild patients (4.27 × 109/L). The levels of IL-6, IL-10, and IFN-γ in patients with adenovirus pneumonia were significantly elevated with the severity of the disease. Compared with the healthy control (20.82%) and the stable patients (33.96%), the percentage of CD8+ T cells that produced IFN-γ increased to 56.27% in the progressing patients. Adenovirus infection increased the percentage of CD8+ T and CD4+ T cells that produce IFN-γ in the co-culture system. The hyperfunction of IFN-γ+ CD8+ T cells might be related to the severity of adenovirus infection. The in vitro co-culture cell model could also provide a usable cellular model for subsequent experiments

The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

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Screening and verification of antiviral compounds against HSV-1 using a method based on a plaque inhibition assay

Abstract Background Herpes simplex virus type 1 (HSV-1) infection is a common viral disease that mainly causes oral lesions, but can also cause genital lesions in some instances. Current treatments with nucleoside analogs are limited by the emergence of drug resistance. Therefore, novel anti-HSV-1 drugs are urgently needed. Methods In this study, we screened a library of 2080 compounds for anti-HSV-1 activity using a plaque formation assay. We selected 11 potential inhibitors of HSV-1 and further evaluated their antiviral effects by plaque reduction assay and real-time polymerase chain reaction (qPCR). Results Five compounds, namely ginsenoside Rd, brassinolide, rosamultin, 3’-hydroxy puerarin, and clinafloxacin HCl, showed potent anti-HSV-1 activity and completely suppressed plaque formation at a concentration of 10 µM. Among them, clinafloxacin HCl, a fluoroquinolone antibiotic, exhibited a high selectivity index for HSV-1. Conclusions Our findings suggest that these five compounds have potential antiviral properties against HSV-1 and may have different mechanisms of action. Further studies are warranted to elucidate the antiviral mechanisms of these compounds and to explore their therapeutic potential for HSV-1 infection

The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

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The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

READM

The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

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The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

fcas

The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

READM

The sulfur isotopic consequence of seawater sulfate distillation preserved in the Neoproterozoic Sete Lagoas post-glacial carbonate, eastern Brazil

params