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Not AvailableOut of twenty seven Gram positive and spore forming Bacilli, four Bacillus thuringiensis (Bt) were obtained from root nodules of six legumes. Three bipyramidal and one spherical crystal producing B. thuringiensis were isolated from root nodules of ricebean, lentil, gahat (Horsegram) and soybean plant. Double Intrinsic Antibiotic Resistance (IAR) markers were developed to detect/monitor the presence of B. thuringiensis in the natural environment. B. thuringiensis isolates were examined for their ability to enhance plant growth and ascending migration (from roots to aerial plant part) in four legumes in plant growth chamber at 28 C. Seed bacterization with B. thuringiensis isolates positively influenced the percent germination and enhanced the plant growth of ricebean, soybean, gahat, and lentil seedlings. All B. thuringiensis isolates were recovered from rhizosphere, root endophytic region, stem lower, upper part and leaf after 45 days after sowing (DAS). Isolate VRB1 was able to colonized rhizosphere and endophytic regions (root, stem and leaf) of gahat, soybean and ricebean after 45DAS. Similarly, isolate VL4C and VLS72.1 were able to colonize rhizosphere and endophytic regions (root, stem and leaf) of lentil and soybean. While, isolate VLG15 was able to colonized rhizosphere and endophytic regions (root, stem and leaf) of lentil, soybean and gahat. Therefore, this study suggests that this approach may be utilized for the development of insect resistant crop strategy in sustainable agriculture.ICA

Fermilab A scheme to extract a low intensity slow spill Main Injector beam to the Meson Area without compromising antiproton production rate

Abstract We propose a scheme to extract a low intensity beam of 120 GeV Main Injector protons to the Meson Area while simultaneously fast extracting protons for antiproton production such that the total antiproton production rate is una ected. We a c hieve this by injecting two booster batches into the Main Injector. At the beginning of at-top, a single booster batch is extracted to the antiproton source. The remaining batch is used to provide a slow spill to the meson area of low i n tensity. At the end of the slow spill, the total amount of beam extracted to meson area is less than 10 of the remaining batch which is extracted to the antiproton source providing two batches for anti-proton production in a period of 3 seconds, thus preserving the rate of antiproton production

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Not AvailableLow temperature proteomic response of psychrotrophic bacterium Pseudomonas lurida NPRp15 was investigated. Culture was subjected to cold shock (28°C to 4°C) and cold acclimatization at 4°C and 15°C. Subsequently bacterial cellular proteins were extracted and separated by SDS-PAGE. Statistical tools (Principal component analysis) were employed to analyze the cold induced and cold acclimatize proteins based on their variations in their expression level. Noticeable variations were observed in protein expression levels for cold shock and cold acclimatized bacterial cells. Similarly PCA analysis clearly classified four groups of proteins depending upon their expression levels. Group I contain 4 proteins that expression level was instantly higher in cold shock and referred as cold shock proteins (Csps). While, Group II contains 5 proteins categorically expressed immediately after cold shock and continuously expressed at the end suggested their role as both cold shock proteins (Csps) and cold acclimation proteins (Caps). Whereas group III contained those proteins whose expression level was not affected or slightly lowered during cold shock. Fourth group proteins expression level was found lowered and suggested their negligible roles in bacterial cold adaptation. The findings of present study indicated that psychrotrophic Pseudomonas lurida NPRp15 expressed considerably some sets unique higher molecular weight cold induced proteins that are playing vital role in bacterial cold adaptation.ICA

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Not AvailableThe effect of a cold tolerant Pseudomonas sp. Strain, PGERs17, on nodulation, iron acquisition and nutrient uptake of field pea (Pisum sativum L. variety VL Matar 42) seedling was determined on the basis of iron acquisition and growth promotion, chlorophyll content, physiologically available iron, Leghaemoglobin from nodules, nitrogen (N) uptake, phosphorus (P) uptake, potassium (K) uptake, iron (Fe), and zinc (Zn) uptake of shoots. Coinoculation of PGERs17 with Rhizobium leguminosarum-PR1 significantly (P > 0.05) increased nodulation (156.2%) and 57.1% higher plant biomass. Coinoculation enhanced total chlorophyll content (31.5%), physiologically available iron (106.7%), total iron (95.9%) and 17.5-fold higher leghaemoglobin concentration in root nodules over uninoculated control plants. Coinoculation also enhance N uptake (66.3%), P uptake (23.3%), K uptake (47.1%), and 2.75-fold higher Zn uptake of shoots compare with uninoculated control. Hence, cold tolerant Pseudomonas sp. strain PGERs17 can be employed as a bioinoculant along with Rhizobium leguminosarum-PR1 to enhance plant growth, iron acquisition and nutrient uptake of field pea enhance plant growth, iron acquisition and nutrient uptake of field pea seedlings at cold temperature conditions.ICA

Effect of pressure and substrate temperature on the deposition of nano-structured silicon–carbon–nitride superhard coatings by magnetron sputtering

A systematic investigation on the deposition of silicon–carbon–nitride (Si–C–N) films under varying deposition conditions such as pressure, substrate temperature and nitrogen content was carried out by radio frequency and direct current magnetron sputtering techniques. Significant role of the different deposition parameters on hardness and structure in the film was observed. It was observed that there was a certain range of nitrogen to argon partial pressure ratio (90:10 to 98:2) for which the particle size was reduced and the films were smooth with fine particle growth, beyond this limit the films had larger particle growth and roughness. The hardness of the deposited film varied between 4400 Hv and 473 Hv depending on deposition condition. Si–C–N film with hardness above 4400 Hv by reactive RF magnetron sputtering from SiC–C composite target in nitrogen–argon was obtained. X-ray diffraction studies revealed the amorphous nature of the deposited films, whereas nano-crystallinity of the particles was noticed during atomic forced microscopy observations. X-ray photoelectron spectroscopy analysis showed the presence of C–N and Si–N bonds in the harder films. It was found that the presence of β-C3N4, Si3N4 and graphite phases and the particle growth in the deposited films control the hardness of the film