1 research outputs found
Detection of Prosthetic Joint Infection Based on Magnetically Assisted Surface Enhanced Raman Spectroscopy
Accurate
and rapid diagnosis of prosthetic joint infection (PJI)
is vital for rational and effective therapeutic management of this
condition. Several diagnostic strategies have been developed for discriminating
between infected and noninfected cases. However, none of them can
reliably diagnose the whole spectrum of clinical presentations of
PJI. Here, we report a new method for PJI detection based on magnetically
assisted surface enhanced Raman spectroscopy (MA-SERS) using streptavidin-modified
magnetic nanoparticles (MNP@Strep) whose surface is functionalized
with suitable biotinylated antibodies and then coated with silver
nanoparticles by self-assembly. The high efficiency of this approach
is demonstrated by the diagnosis of infections caused by two bacterial
species commonly associated with PJI, namely, <i>Staphylococcus
aureus</i> and <i>Streptococcus pyogenes</i>. The method’s
performance was verified with model samples of bacterial lysates and
with four real-matrix samples of knee joint fluid spiked with live
pathogenic bacterial cells. This procedure is operationally simple,
versatile, inexpensive, and quick to perform, making it a potentially
attractive alternative to established diagnostic techniques based
on Koch’s culturing or colony counting methods