Antioxidant Capacities and Phenolic Levels of Different Varieties of Serbian White Wines

The biologically active compounds in wine, especially phenolics, are responsible for reduced risk of developing chronic diseases (cardiovascular disrease, cancer, diabetes, etc.), due to their antioxidant activities. We determined the contents of total phenolics (TP) and total flavonoids (TF) in selected Serbian white wines by colorimetric methods. Total antioxidant activity (TAA) of the white wines was analyzed using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging capacity assay. Međaš beli had the highest content of TP, TF and TAA. The radical scavenging capacity (RSC) and total antioxidant activity (TAA) of white wines were 15.30% and 1.055 mM Trolox equivalent, respectively. Total phenolic (TP) and total flavonoid (TF) contents in white wines ranged from 238.3 to 420.6 mg gallic acid equivalent per L of wines and 42.64 to 81.32 mg catechin equivalent per L of wines, respectively. A high and significant correlation between antioxidant activity and total phenolic content was determined in wines (R2 = 0.968, p < 0.01). For the individual polyphenols determination we used a high performance liquid chromatography (HPLC)-diode array detection (DAD) technique. The majority of white wine polyphenols was represent by four hydroxycinnamic acids (HCAs)

Editorial: Non-coding RNA in diabetes and cardiovascular diseases

Editorial on the Research Topic: [https://www.frontiersin.org/research-topics/33365/non-coding-rna-in-diabetes-and-cardiovascular-diseases

Polyphenol Content and Antioxidant Activity of Sour Cherries From Serbia

The aim of this study was to evaluate the content of phenolics: the total phenols (TP), flavonoids (TF), anthocyanins (TA), as well as the total antioxidant\ud capacity (TAC) in three sour cherry cultivars (Prunus cerasus L.) introduced to the southeast Serbia climate conditions. Among the investigated sour cherries,\ud „Oblačinska“ cultivar contained the highest amounts of all groups of phenolics, followed by „Cigančica“ > „Marela“. A significant difference were observed in the phenolic content among different cultivars and growing seasons (p  0.05), and the phenolic compounds were significantly higher in the growing season 2009. The examined cultivars possess a high antioxidant capacity, and all phenolics of highy correlation with TAC. The following compounds were identified and quantified using HPLC-DAD: 4 anthocyanins, the most abundant of which was cyanidin-3-glucoside in “Marela” and “Oblačinska”, and cyanidin-3-glucosylrutinoside in „Cigančica“, and 4 hydroxycinnamic acids, the most abundant of which was neochlorogenic acid in all sour cherry cultivars. The growing and ripening process on the tree of sour cherry cv. „Oblačinska“ was evaluated also. The results showed significant increases in total phenols during the ripening, the total anthocyanins and total antioxidant capacity and 4 quantified anthocyanins, however the neochlorogenic acid decreased during the ripening. The study indicated that the growing and climate conditions in southeast Serbia are convenient for introducing sour cherry cultivars.\u

Karakterizacija sojeva Xanthomonas euvesicatoria, patogena paprike u Srbiji

During spring and summer of 2008, 101 bacterial strains was isolated from the diseased pepper leaves collected from different pepper growing areas in the Republic of Serbia. The aim of this research was to characterize the isolated strains and determine their taxonomic position according to the most recent nomenclature. Pathogenic, biochemical and physiological characteristics of isolated bacteria were tested using standard bacteriological tests. The pathogen races were determined according to the reaction of differential varieties of Early Calwonder (ECW), their isogenic lines (ECW-10R, ECW-20R, ECW-30R) and Capsicum pubescens. The sensitivity of strains to bactericides was studied in vitro by culturing bacteria on sucrose pepton agar (SPA) plates, amended with filter-sterilized aqueous solution of streptomycin and kasugamycin (50, 100, 200 ppm) or copper-sulphate (100, 200 ppm). Based on pathogenic, biochemical and physiological characteristics, the investigated strains belonged to Xanthomonas euvesicatoria. The reaction of pepper differential varieties indicated that these strains belonged to pepper races P1, P3, P7, P8. Streptomycin resistant strains were not detected, but 6 strains were resistant to kasugamycin (50 ppm) and 13 strains to copper-sulphate (200 ppm), indicating bacterial resistance development.Tokom 2008. godine prikupljeni su uzorci obolelog lišća paprike sa simptomima bakteriozne pegavosti iz različitih lokaliteta Republike Srbije. Izolacijom iz zaraženih listova dobijen je 101 soj bakterija. Cilj ovog rada je proučavanje odlika i identifikacija izolovanih sojeva bakterije u skladu sa najnovijom nomenklaturom. Patogene i biohemijsko-fiziološke karakteristike sojeva proučene su korišćenjem standardnih bakterioloških testova. Određivanje fizioloških rasa bakterijske populacije izvršeno je na osnovu reakcije diferencijalnih sorti paprike Early Calwonder (ECW), njenih izogenih linija (ECW-10, ECW-20 ECW-30) i reakcijom sorte Capsicum pubescens. Proučena je osetljivost sojeva u in vitro uslovima na streptomicin, kasugamicin i bakar-sulfat. Rezultati proučavanja biohemijsko-fizioloških odlika sojeva ukazuju da naši sojevi pripadaju genetičkoj grupi „A“, odnosno vrsti Xanthomonas euvesicatoria. Proučavani sojevi, takođe, predstavljaju heterogenu populaciju u kojoj su zastupljene četiri fiziološke rase bakterije X. euvesicatoria (P1, P3, P7, P8). Sojevi rezistentni na streptomicin nisu detektovani ovim istraživanjima. Otpornost na 50 ppm kasugamicina utvrđena je kod 6 sojeva, a 13 sojeva je bilo otporno na 200 ppm bakar-sulfata. Prikazani rezultati ukazuju na opasnost od razvoja rezistentnosti bakterija na ova jedinjenja

Zuclopenthixol decanoate in pregnancy: Successful outcomes in two consecutive off springs of the same mother

Introduction. Almost all individual antipsychotics are classified into the intermediate pregnancy risk category as no or limited data exist about human pregnancy outcomes. We presented the case of zuclopenthixol decanoate using in two successive pregnancies of the same woman, which had not been published in the available peer-reviewed literature. Case report. A middle-age female subject who suffered from schizophrenia received zuclopenthixol decanoate injection during her two consecutive pregnancies. About four and a half months before diagnosis of the first pregnancy (~3.5 years after psychosis emergence), zuclopenthixol decanoate (400 mg every other week, im injection) was introduced to the treatment protocol (due to previous non-compliance with halo-peridol and risperidone). A significant clinical improvement was achieved and the dose during pregnancy was reduced to 200 mg once monthly and maintained to date. In both pregnancies the women gave birth to healthy girls who have been developing normally until now, at their ages of 6 months and of 3.5 years. During pregnancy and after giving birth to children the mothers' psychiatric status and her social functioning were significantly improved and are still stable. Close monitoring of the mother's health, a multidisciplinary approach to both her treatment and the monitoring of pregnancies as well as the complete compliance with the prescribed drug protocol were likely to be crucial for the therapeutic success. Conclusion. A favorable outcome of the present case suggests that the zuclopenthixol decanoate is a rational therapeutic option for pregnant women suffering from psychosis when the expected benefit exceed the potential risk, but a definitive evidence for its safety requires large, controlled studies

Nitric oxide, thyroglobulin, and calcitonin: unraveling the nature of thyroid nodules

BackgroundThyroid nodules (TN) are localized morphological changes in the thyroid gland and can be benign or malignant.ObjectiveThe present study investigates the relationships between biochemical markers in serum (s) and their homologs in washout (w) after fine-needle aspiration biopsy (FNAB) of the TN of interest and their correlation with cytology specimen findings.MethodsWe investigated the relationships between serum biochemical markers nitric oxide (NO), thyroglobulin (TG), and calcitonin (CT), their homologs in washout after FNAB of the TN of interest, and cytology findings of biopsy samples classified according to the Bethesda system for thyroid cytopathology in this study, which included 86 subjects.ResultsWashout TG (TGw) level positively correlates with the cytology finding of the biopsy. A higher level of TGw correlates with higher categories of the Bethesda classification and indicates a higher malignant potential. The levels of serum NO (NOs), serum TG (TGs), serum CT (CTs), and washout CT (CTw) do not correlate with the cytology finding of the biopsy, and the higher levels of washout NO (NOw) correspond to the more suspicious ultrasound findings.ConclusionThe findings of our study suggest that TGw and NOw could be used as potential predictors of malignancy in TN

Conductometric and pH metric investigations of the oxalic acid and NaAsO2 reaction

The reaction between NaAsO2 and oxalic acid was studied by pH-metric and conductometric measurements, applying the methods of continual variation and pH-metric and conductometric titration. It was found that oxalic acid forms a complex anion of the type [AsOC2O4]-. The relative stability constant of the complex at ionic strengths, I, of 0.10 (log Kr = 4.70), 0.20 (log Kr = 4.51), 0.50 (log Kr = 4.24) and 0 (log K0r = 5.05) and thermodynamic para¬meters were calculated using the data obtained by pH-metric measurements at 25.0±0.1 °C (DH = 10.5 kJ mol-1, DG = –29.0 kJ mol-1, DS = 133 J mol-1 K-1)