24 research outputs found
Raw data for review on the effects of toxicants on freshwater ecosystem functions
Raw data for review on the effects of toxicants on freshwater ecosystem function
The experimental set-up.
<p>The flow-through testing apparatus, showing four experimental units (volume, 500 mL) with five <i>D. magna</i> each. Approximately 40 mL of the test medium was introduced every other hour slightly below the surface of the water. The old test medium was passively discharged using hydrostatic pressure at the bottom of each vessel. A fine mesh screen (0.1 mm) prevented the loss of recently hatched juvenile daphnids.</p
Titanium Dioxide Nanoparticles Increase Sensitivity in the Next Generation of the Water Flea <em>Daphnia magna</em>
<div><p>The nanoparticle industry is expected to become a trillion dollar business in the near future. Therefore, the unintentional introduction of nanoparticles into the environment is increasingly likely. However, currently applied risk-assessment practices require further adaptation to accommodate the intrinsic nature of engineered nanoparticles. Combining a chronic flow-through exposure system with subsequent acute toxicity tests for the standard test organism <em>Daphnia magna</em>, we found that juvenile offspring of adults that were previously exposed to titanium dioxide nanoparticles exhibit a significantly increased sensitivity to titanium dioxide nanoparticles compared with the offspring of unexposed adults, as displayed by lower 96 h-EC<sub>50</sub> values. This observation is particularly remarkable because adults exhibited no differences among treatments in terms of typically assessed endpoints, such as sensitivity, number of offspring, or energy reserves. Hence, the present study suggests that ecotoxicological research requires further development to include the assessment of the environmental risks of nanoparticles for the next and hence not directly exposed generation, which is currently not included in standard test protocols.</p> </div
The sensitivity of juveniles released during the third set of experiments.
<p>96 h- EC<sub>50</sub> values with respective 95% CIs of the fifth brood released by adults exposed to 0.00 and 2.00 mg/L P25-nTiO<sub>2</sub> during the third set of experiments, which considered exclusively potential implication of nTiO<sub>2</sub> exposure within the brood pouch. No statistically significant difference among treatments was observed.</p
The sensitivity of juveniles released by nTiO<sub>2</sub>-exposed adults.
<p>The cumulative mean (±95% CIs) difference in sensitivity â in terms of 96 h-EC50 values â of the offspring (fifth brood) released by adults exposed to 0.02 or 2.00 mg nTiO2/L and offspring released by control (uncontaminated) daphnids is displayed using the standardised effect size Cohenâs <i>d</i>. (<i>A</i>) The cumulative effect sizes for all bioassays conducted (nâ=â7) with the fifth brood during the first and second set of experiments. (<i>B</i>) The cumulative effect sizes for acute toxicity experiments conducted with offspring released in the control medium by adults previously exposed to the above-mentioned nTiO2 concentrations during the second set of experiments (nâ=â3). The statistical significance of a cumulative effect is highlighted by an asterisk (*). Negative effect sizes indicate increased toxicity.</p
Heavy Metal Uptake and Toxicity in the Presence of Titanium Dioxide Nanoparticles: A Factorial Approach Using <i>Daphnia magna</i>.
Unintentionally
released titanium dioxide nanoparticles (nTiO<sub>2</sub>) may co-occur
in aquatic environments together with other
stressors, such as, metal ions. The effects of P25-nTiO<sub>2</sub> on the toxicity and uptake of the elements silver (Ag), arsenic
(As) and copper (Cu) were assessed by applying a factorial test design.
The test design consisted of two developmental stages of <i>Daphnia
magna</i>, two levels of nTiO<sub>2</sub> (0 versus 2 mg/L) as
well as seven nominal test concentrations of the respective element.
The presence of nTiO<sub>2</sub> increased Ag toxicity for juveniles
as indicated by a 40% lower 72-h EC<sub>50</sub>, while the toxicities
of As and Cu were reduced by up to 80%. This reduction was even more
pronounced for Cu in the presence of dissolved organic carbon (i.e.,
seaweed extract) and nTiO<sub>2</sub>. This outcome coincides with
the body burden of the elements, which was elevated 2-fold for Ag
and decreased 14-fold for Cu in the presence of nTiO<sub>2</sub>.
Although the underlying mechanisms could not be uncovered, the data
suggest that the carrier function of nTiO<sub>2</sub> plays a central
role. However, to understand the processes and mechanisms occurring
in the field due to the presence of nTiO<sub>2</sub> further systematic
investigations considering environmental variables and nanoparticle
characteristics are required
The sensitivity of juveniles released by adults exposed to different nTiO<sub>2</sub> products.
<p>Sensitivity, displayed as percent relative to the 96 h-EC<sub>50</sub> of the respective control, of the fifth brood released by adult <i>D. magna</i> exposed to different nTiO<sub>2</sub> treatments using the products P25 or A-100. The data displayed for P25 represent the weighted mean values of the seven experiments (first and second set of experiments), each with four replicates per treatment, whereas the 96 h-EC<sub>50</sub> for the offspring released from the control parents was 3.13 mg/L nTiO<sub>2</sub>. For the product A-100, the results of one experiment with four replicates of pre-treatment are displayed (fourth set of experiments). In this situation, the 96 h-EC<sub>50</sub> for the offspring released from the control parents was 1.98 mg/L nTiO<sub>2</sub>. The error bars and dashed lines indicate the standard error. The dashed lines are related to the control. Asterisks (*) denote significant differences between a treatment and the respective control.</p
Scanning electron microscope image.
<p>An image of the size-homogenised, stable nTiO<sub>2</sub> suspension of the product P25 taken by an scanning electron microscope using 100,000-fold magnification (Hitachi SU8030).</p
Experimental design.
<p>(<i>A</i>) Schematic diagram illustrating the experimental procedure of each of the five sets of experiments conducted. (<i>B</i>) Visualised experimental procedure for the assessment of the âearly exposureâ hypothesis (second set of experiments).</p
Modeling Remobilization of Neonicotinoid Residues from Tree Foliage in StreamsîžA Relevant Exposure Pathway in Risk Assessment?
Systemic
neonicotinoid insecticides are increasingly used as a
crop protection measure to suppress insect pests on trees. However,
senescent foliage falling from treated trees represents a rarely studied
pathway through which neonicotinoids may enter nontarget environments,
e.g., surface waters. To estimate risk posed by this pathway, neonicotinoid
residues were analyzed in foliage from black alder trees treated with
one of three neonicotinoid insecticides (imidacloprid, thiacloprid,
or acetamiprid) at five concentrations, each ranging from 0.0375â9.6
g active ingredient/cm trunk diameter at breast height (<i>n</i> = 3). Foliar residues measured at the time of leaf fall were used
as input parameters for a model predicting imidacloprid water concentrations
over a 100-m-long stream stretch as a consequence of remobilization
from introduced foliage (input: 600 g foliage/m<sup>2</sup> containing
80 ÎŒg imidacloprid/g). The water concentration (up to âŒ250
ng/L) predicted by the model exceeded the recently proposed Maximum
Permissible Concentration of 8.3 ng/L for âŒ6.5 days. Moreover,
dietary uptake was identified as an additional exposure route for
aquatic organisms. The alternative pathway (i.e., introduction via
leaf fall) and exposure route (i.e., dietary uptake) associated with
the systemic nature of neonicotinoids should be accounted for during
their registration process in order to safeguard ecosystem integrity