Identification of Trypanorhyncha parasite from green tiger shrimp larvae in Bushehr Province coastal waters

In this study, 37 gut contents (22 females and 15 males) green tiger shrimp (Penaeus semisulcatus) from point of parasite infection were studied, The results showed that infection frequency in female and male were 72 and 86%, respectively, and there was no Significant difference between two species from point of reaction to infection. Furthermore, there is no relations between size of Shrimp and infection prevalence. The most important point is that the Trypanorhyncha cestodes showed a high distribution all along the Bushehr province coastal waters in the North West of Persian Gulf

Screening and evaluation of indigenous bacteria from the Persian Gulf as a probiotic and biocontrol agent against Vibrio harveyi in Litopenaeus vannamei post larvae

Isolation of autochthonous bacteria from marine sources as a potential probiont in biocontrolling against pathogenic Vibrio species in the shrimp culture industry was the aim of current research. A total of 198 bacterial strains were isolated from pond water, sediment, hepatopancreas and gut samples of shrimps after culturing the samples on Tryptic Soy Agar and incubated at 30 °C for 24-48 h. The isolates were tested for their antagonistic activity in contact with Vibrio harveyi. Two strains (IS02 and IS03) that isolated from the gut and pond sediment were showed antagonistic against V. harveyi. According to 16S ribosomal DNA gene sequence analysis, the strain IS02 was identified as Bacillus subtilis and IS03 as B. vallismortis. Further, the two bacterial species, B. subtilis and B. vallismortis were challenged separately for probiotic activity in the post larvae of Litopenaeus vannamei against pathogenic V. harveyi. The present study identified B. subtilis IS02 and B. vallismortis IS03 had biocontrol activity against V. harveyi in vitro and in vivo and they increase growth performance of L. vannamei in post larvae stage

Risk assessment in the laboratories of the research centers affiliated to Iranian Fisheries Research Organization

Identification of risks in laboratories and trying to create safe conditions is very important from different aspects. The main objective of this study was to evaluate the potential risks in the laboratories of three research centers affiliated to Iranian Fisheries Science Research Institute. In order to assess and classify risks associated with working in the laboratories (11 laboratories of the Persian Gulf and Oman Sea Ecological Research Center, 9 laboratories of National Shrimp Research Center and 2 laboratories of National Aquatic Organisms Processing Center), the method of "Failure Mode Effects Analysis" (FMEA) as well as some statistical methods were used. The risk levels in all the laboratories of the Persian Gulf and Oman Sea Ecological Research Center, except for benthos laboratory, could be evaluated as moderate or high. Only in the case of the sample preparation laboratory, significant differences between the values of RPN before and after corrective action could be observed. However, in this case the corrective actions have not been effective in decreasing the risk level. In most laboratories of National Shrimp Research Center, the corrective actions were effective in reducing the risk levels (with the exception of three laboratories including contaminants, microbiology and chemistry-physics). In both laboratories of National Aquatic Organisms Processing Center, after the corrective actions, the risk levels have been sharply reduced. In general it can be concluded that FMEA is an effective method for risk assessment in the research laboratories and appropriate statistical methods can also be used for complementary analysis

Effects of aflatoxin B1 on growth performance, health indices, phagocytic activity and histopathological alteration in Fenneropenaeus indicus

Mycotoxins contamination of feedstuff for aquatic animals is common in regions with humid tropical conditions. In this study Indian white shrimp, Fenneropenaeus indicus, (11.79 ± 1.76 g) were fed with diets containing 0, 20, 50, 100, 200, 400, 800 and 1600 ppb levels of aflatoxin B1 (AFLB1) for 8 weeks. Final weight, aflatoxin B1 residue (2-week intervals), Total Hemocyte Count (THC), Total Plasma Protein (TPP), Phagocytic Activity (PA), Survival rate (4-week intervals) were determined. Histopathological alterations in hepatopancreas, midgut and muscle tissues were studied at the end of 4 and 8 weeks. Shrimps fed with the 1600, 800 and 400 ppb concentrations of AFLB1 exhibited slow growth, and more reddish discoloration disseminated over the body at 4th week. Growth parameters, survival rate and health indices (THC, TPP) of F. indicus, are affected by the different doses of AFLB1 in diets. At the end of 8th week, doses of AFLB1 in the diets showed negative correlation to final weight, survival rate, THC and TPP (r = - 0.312, -0.603, -0.237 and - 0.649 at P<0.001, respectively). Moreover, significant histopathological alterations in the hepatopancreas, midgut and muscle tissues of exposed shrimps to different levels of AFLB1 were observed and these alterations are obviously indicated by changes in the health indexes (THC and TPP)

Screening of pathological agent and recognize of disease in shrimp specific pathogen free (SPF) production

The production of specific pathogen free (SPF) of native shrimp is the first step for sustainable shrimp culture in Iran. This concept needs better understanding of health managmet and biosecurity in shrimp broodstock production for produc 25000MT in the five programe and development of Iran. In SPF center the main pathogen consist of viruses, bacteria and fungei that will be exclude the shrimp SPF.Theses pathogen divided to three categories, the first category consist of highly virlent pathogen such as white spot syndrome virus, Tura syndrome virus, Infection myonecrosis virus, infection hypodermal and hematopoietic virus. The second category are hepatopancratuc parvo like virus, and vibrio bacteria, Necrotizing hepatopnacratits, microsporidia and haplosporidia and the third category consist gregarines with light virlent.In the high level project that conducted in Iran between 2011 untill now about 8000 brodstoock collectd from farm culture in bousher province during September and October of 2012 from high health and Moluky stock. All shrimp tested for viruse, bacteria and fingai and the results showed the shrimp wrer free of viruse and bacterial pathogen. During the study one sample showed necrotizing hepatopancreatitis and we removed from the center

Effect of hot-water extract of brown seaweed Sargassum glaucescens via immersion route on immune responses of Fenneropenaeus indicus

The development of shrimp aquaculture, in spite of its global necessity, is largely at stake as significant ecological and pathological problems are increasing in the vast majority of the shrimp producing countries. Shrimp immunology is a key element in establishing strategies for controlling diseases in shrimp aquaculture. The total haemocyte count (THC), differential haemocyte count (DHC), total plasma protein (TPP), Phagocytic activity (PA), bacterial clearance efficiency (BCE) and bactericidal activity (BE) were examined when the F. indicus shrimps (11.32±1.20 g) were immersed in seawater (39 ppt and 25 ± 1 ºC) containing hot-water extracts of brown alga Sargassum glaucescens at 100, 300 and 500 mg/l. These parameters increased significantly (p < 0.05) when the shrimp were immersed in seawater containing hot-water extracts at 100 mg/l after 3h and 300 and 500 mg/l after 2 h. F. indicus shrimps that were immersed in hot-water extracts at 300 and 500 mg/l had increased phagocytic activity and clearance efficiency to Vibrio spp. after 2 hours. But bactericidal activity increased significantly after 1 hour in the same concentrations

On Cross-Domain Transfer in Venue Recommendation

Venue recommendation strategies are built upon Collaborative Filtering techniques that rely on Matrix Factorisation (MF), to model users’ preferences. Various cross-domain strategies have been proposed to enhance the effectiveness of MF-based models on a target domain, by transferring knowledge from a source domain. Such cross-domain recommendation strategies often require user overlap, that is common users on the different domains. However, in practice, common users across different domains may not be available. To tackle this problem, recently, several cross-domains strategies without users’ overlaps have been introduced. In this paper, we investigate the performance of state-of-the-art cross-domain recommendation that do not require overlap of users for the venue recommendation task on three large Location-based Social Networks (LBSN) datasets. Moreover, in the context of cross-domain recommendation we extend a state-of-the-art sequential-based deep learning model to boost the recommendation accuracy. Our experimental results demonstrate that state-of-the-art cross-domain recommendation does not clearly contribute to the improvements of venue recommendation systems, and, further we validate this result on the latest sequential deep learning-based venue recommendation approach. Finally, for reproduction purposes we make our implementations publicly available

An Investigation on the possibility of utilization of chemical material for mitigation of Cochlodinium sp. bloom and their impact on the Litopenaeus vannamei shrimp

Cochlodinium polykrikoides is a common red tide former associated with fish kills in the most coastal waters of the world. Pay attention red tide problems to human and environment, forecast and control of algal bloom are aim of a lot of coastal environmental studies. Following Cochlodinium polykrikoideswas bloom in Oman Sea and Persian Gulf, extensive mortality of aquatic animal were seen in 2008, instances verified extensive damaged to wetland habitats during algal bloom. In this study have been tried for finding a suitable chemical composition for Mitigation of Cochlodinium polykrikoides bloom in wetlands. To identify the safe chemical substance against red tide in coastal waters wetlands, concentration effects of 0.01 mg/l, 0.04 mg/l, 0.4 mg/l, 1 mg/l and 1 gr/l Magnesium Hydroxide, Poly Aluminum Chloride (PAC), Aluminum Sulfate, Sodium Hypochlorite, Calcium Carbonate, Ferrous Sulfate, Starch on Laboratory cultures of Cochlodinium polykrikoides (1000000 cell/lit) and Litopenaeus vannamei were studied in Iranian Shrimp research center. Cochlodinium polykrikoides were cultured under a cool white fluorescent light of 2000 lux intensity with a 12:12 h light: dark cycle. Temperature and salinity were controlled 28 °C and of 30 g/l (ppt), respectively. Finding show that, all concentration of above substance, completely disintegrated of cultured Cochlodinium polykrikoides. Also except 1 g/l Sodium Hypochlorite, in other concentrations of studied substances, there weren’t seen shrimp mortality after 96h. Among studied substance, starch have limit effects on the shrimp health and environment, therefore it can be suitable for controlling of Cochlodinium polykrikoides in shrimp ponds

The سtudy on health situation of shrimp Litopenaus vannamei broodstoock production in earthen pond and comparing with fiberglass or cement tanks

The investigation of health and diseas situation of shrimp broodstook (Litopenaeus vannamei) in Boushehr province in earthen pond and cancreat and fiberglas was carried out from May 2014 until July 2014 with collected 100 samples brood stook from earthen pond and 100 samples from earth and fiberglas thank.The clinical sign of samples documented in take history form and then the samples transport to Iran Shrimp Research Instituet in Bousheher. The bacterial and fungal studied was carried out with hemolymp, hepatopacreas and gill tissue and then the shrimp preserved in Davidson Fixative for histopathology. A part of uropoda also preserved in ethyl alcholo for PCR study and detecting viruses. The result showed 10 bacteria consist V.harveyii, V.parahaemolyticus, V.anguillarum, V.vulnificus, V.mimicus, V.damsela,V.nereis, plesiomonas shigelloides V.alginolyticus V.proteolyticus, in earthen pond and 5 bacteria consit ,V.alginolyticus V.proteolyticus ، V.parahaemolyticus، V. damsela ،V.mimicus were identified in fiberglas thank. In this study 10 fungi consist Penecilium, Asp. Niger, Asp. Flavious, Asp. fomigatus, Acromonium, Ulocladium, Mucor, Cladosporium, Alternaria, Rhizopus, and 5 of them were identified in both broodstock from earthen pond and fiberglas thank. However 6 parasite consist of zoothamnium, vorticella, Acneta, Ephelota, Epistylis, Epistylis and Apastomom identified in earthen pond and three of them were identified in fiberglas thank. In histoplatholgy some tissue showed the effect of vibrio infecting in different organs as well in gill and midgut and the PCR examined were negative for all viruses. Regarding the produce healthy broodstock we need excuted the High Health procdure

Monitoring and identification of viral pathogens in SPF (Specific Pathogen Free) shrimp production project

Shrimp aquaculture has developed rapidly in 1980s decade and this development caused to increase shrimp diseases in farms. In the last 0f 1980s and first of 1990s find of health shrimp Postlarvae for stocking was hard. In Iran also after two decades diseases are the main problems, then a national project (Shrimp SPF production) was performed that this section was monitoring and identification of viral pathogens in that project. For this propose selected shrimps in farms was sampled and screened for main viruses (OIE list), shrimps without infection bring to quarantine system for one month and in the end of quarantine period were screened again. Then in winter spending, before and after spawning, it’s offspring in F1 and F2 also screened for viral pathogens by IQ2000 kit in Iranian veterinary organization lab. Moreover all shrimp feeds in clouding: fresh feed, live feed and pellet feed had been screened for OIE viral pathogens listed for three years. Results of viruses screening in shrimp sampled in all stages of project and feed that use in this project were negative. This project (SPF shrimp production in IRAN) performed successfully, we think this success relate to biosecurity system that were influence in this project