qRT-PCR summary statistics on biological samples collected from the three surveyed Australian <i>Pteropus</i> species.

<p>¹Packed haemocytes</p><p>qRT-PCR summary statistics on biological samples collected from the three surveyed Australian <i>Pteropus</i> species.</p

qRT-PCR Ct results on biological samples, and physiological characteristics of 42 HeV-positive Black flying-foxes (<i>P</i>. <i>alecto</i>)¹.

<p>¹All 42 HeV-positive flying-foxes in the study were Black flying-foxes (<i>P</i>. <i>alecto</i>) caught at the Boonah roost.</p><p>²M = Male; F = Female: A = Adult; SA = Sub adult; J = Juvenile: G = Gestation; L = Lactation; P = Pup attached: Urogenital = vulvovaginal swab in females and preputial swab in males; PHemo = Packed haemocytes.</p><p>qRT-PCR Ct results on biological samples, and physiological characteristics of 42 HeV-positive Black flying-foxes (<i>P</i>. <i>alecto</i>)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140670#t003fn001" target="_blank">¹</a>.</p

qRT-PCR status summary statistics on biological samples from 42 HeV-positive Black flying-foxes (<i>P</i>. <i>alecto</i>)¹.

<p>¹All 42 HeV-positive flying-foxes in the study were Black flying-foxes (<i>P</i>. <i>alecto</i>) caught at the Boonah roost.</p><p>²PHemo = packed haemocytes.</p><p>³ Urine samples were collected from 29/42 confirmed positive black flying-foxes.</p><p>⁴ Urogenital samples were collected from 32/42 confirmed positive black flying-foxes.</p><p>qRT-PCR status summary statistics on biological samples from 42 HeV-positive Black flying-foxes (<i>P</i>. <i>alecto</i>)<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140670#t004fn001" target="_blank">¹</a>.</p

Details of 2840 flying-foxes captured and sampled in the eastern Australian states of Queensland (QLD) and New South Wales (NSW) between February 2012 and June 2014.

<p>¹BFF = Black flying-fox (<i>Pteropus alecto</i>); GHFF = Grey-headed flying-fox (<i>P</i>. <i>poliocephalus</i>); LRFF = Little red flying-fox (<i>P</i>. <i>scapulatus</i>).</p><p>² Roosts shared with a structured longitudinal study investigating HeV infection and transmission dynamics in flying-foxes (reported elsewhere); site selection changed after first bi-monthly sampling event from Toowoomba to Boonah.</p><p>³ Roosts shared with a cross-sectional study series investigating the association between flying-fox roost disturbance and HeV infection dynamics [<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0140670#pone.0140670.ref025" target="_blank">25</a>].</p><p>Details of 2840 flying-foxes captured and sampled in the eastern Australian states of Queensland (QLD) and New South Wales (NSW) between February 2012 and June 2014.</p

Classification of flying-fox demographic, morphometric and reproductive variables.

<p>¹ Adult males distinguished from sub-adult males on the basis of fully developed penis and testes. Adult females distinguished from sub-adult females on the basis of worn, elongated nipples, indicating that they had suckled at least once in their lifetime. Females that were palpably pregnant but with no evidence of worn, elongated nipples were classified as adults. Juveniles (< 12 months old) were classified on their smaller size and rudimentary development of sexual characteristics.</p><p>² Assessed primarily through palpation of the pectoral muscle mass and associated prominence of the sternal carinum; quantified on a 5-point scale.</p><p>³ Pregnancy was assessed through gentle trans-abdominal palpation of a gravid uterus (early–mid-pregnancy) or foetal structures (late pregnancy). Dependent pup is in reference to an adult female flying-fox with a totally dependent pup attached, and indicating the early post-partum period. Lactation (in the absence of a dependent pup) was confirmed through gentle expression of milk from enlarged nipples, and indicated the later post-partum period associated with a semi-independent juvenile.</p><p>Classification of flying-fox demographic, morphometric and reproductive variables.</p