Sera from Visceral Leishmaniasis Patients Display Oxidative Activity and Affect the TNF-αProduction by MacrophagesIn Vitro

Submitted by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-05T17:58:16Z No. of bitstreams: 1 Soares NM Sera from Visceral Leishmaniasis Patients....pdf: 712177 bytes, checksum: d58fde5167450c1a9b12e7e1afe5c0d2 (MD5)Approved for entry into archive by Ana Maria Fiscina Sampaio ([email protected]) on 2018-04-05T18:11:20Z (GMT) No. of bitstreams: 1 Soares NM Sera from Visceral Leishmaniasis Patients....pdf: 712177 bytes, checksum: d58fde5167450c1a9b12e7e1afe5c0d2 (MD5)Made available in DSpace on 2018-04-05T18:11:20Z (GMT). No. of bitstreams: 1 Soares NM Sera from Visceral Leishmaniasis Patients....pdf: 712177 bytes, checksum: d58fde5167450c1a9b12e7e1afe5c0d2 (MD5) Previous issue date: 2017Universidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Faculdade de Farmácia. Departamento de Análises Clínicas e Toxicológicas. Salvador, BA, BrasilMammalian protection against leishmanial infection depends on the development of an effective immune response. Zoonotic visceral leishmaniasis (ZVL) patients are usually unable to mount an effective immune response against the parasite and indeed appear to be severely immunosuppressed. This suppression has strong nonspecific and specific components mediated by serum factors and leishmanicidal activity of infected macrophages, respectively. The lipid profile has been shown to be altered in ZVL patients' sera. This work aimed at (i) determining the HDL, Apo A1, LDL, and VLDL concentrations in ZVL patients' sera; (ii) investigating the oxidative effect of ZVL patients' sera on theβ-carotene matrix; (iii) measuring IL-10, IL-6, IL-12p40, and tumour necrosis factor-α(TNF-α) concentrations in the macrophage cultures, to which 10% of ZVL patients' serum had been added. Levels of HDL, LDL fraction, and apolipoprotein A1 in ZVL patients' sera were lower than those of healthy individuals' sera, except for the mean level of VLDL. The matrix ofβ-carotene and linoleic acid system was oxidized in the presence of ZLV patients' sera. The presence of ZVL patients' sera did not modify the cytokine production of IL-6, IL-12p40, and IL-10 by human macrophagesin vitrobut TNF-αproduction was altered, probably due to lack of macrophage stimulation by lipoprotein