Transduction of p27 to Induce Apoptosis in Tumor Cells

Transduction is a biochemical technique for the introduction of full-length proteins into the cells. It has the potential to be used in the development of a new therapeutic strategy for cancer therapy. Different forms of p27 (TAT-p27, TAT-Mp27, TAT-p23) were transduced into tumor cell lines, lymphocytes and B-CLL cells, and their influence on proliferation and apoptosis was investigated. The metabolism of transduced proteins differed between the cell types. TAT-p27 protein is metabolized faster than the mutated form. Furthermore, the half-life of TAT-p27 depended on the type of cells. All forms of TAT-p27 fusion protein moderately decreased the proliferation of different types of the cells and induced apoptosis. The cells from some B-CLL patients were sensitive to TAT fusion proteins, and the sensitivity was increased with the addition of Fluda. This study provides valuable results for further development of TAT technology as the potential tool for a specially targeted therapy of tumors

Drug Delivery by TAT-technology

TAT technology is a biochemical technique for introduction of full-length peptides or proteins into cells. This process occurs in a rapid, concentration-dependent fashion that appears to be independent of receptors and transporters. It has broad implications in experimental systems for regulating intracellular processes and has the potential to be used in the development of new therapeutic strategies for cancer, infectious diseases, and development of vaccines. It has been shown that different forms of TAT-p27 protein can modulate the cell cycle of cultured cell lines, depending on the concentration and type of cells. Transfer of TAT-proteins/peptides use from cell culture systems to animal disease models has been slow, but the ability of TAT conjugates to protect mice against ischemia, inhibit tumor growth, and enhance gene delivery suggests that they offer wide ranging pharmaceutical applications for treating a whole range of diseases

Antibakterijsko djelovanje glikolipoproteina G-90 iz gujavica.

G-90 is a glycolipoprotein mixture obtained from the tissue homogenate of earthworm Eisenia foetida (Annelida, Lumbricidae) which exhibits pleiotropic biological functions. The antibacterial activity of G-90 was investigated in vitro and in vivo. Concentrations of 10 ng/mL and 10 μg/mL of G-90 exhibited an inhibitory effect on in vitro growth of non-pathogenic and facultative-pathogenic bacteria. The bacteriostatic effect of the G-90 was 21% stronger for facultative-pathogenic bacteria than that observed for non-pathogenic bacteria. The analysis of CFU/mL values following swabbing of surgical wounds in mice demonstrated that G-90 used in either 10 ng/mL or 10 μg/mL concentrations inhibited in vivo growth of the bacteria obtained from these wounds. The antibacterial activity of G-90, as well as the biological characteristics exhibited by the preparation, may be of interest for clinical investigations in veterinary and human medicine.G-90 je glikolipoproteinska smjesa dobivena iz tkivnog homogenata gujavica Eisenia foetida (Annelida, Lumbricidae) koja pokazuje brojne biološki značajne aktivnosti. U ovom radu istražena je antibakterijska aktivnost G-90 in vitro i in vivo. G-90 u koncentracijama od 10 ng/mL i 10 μg/mL inhibira rast nekih nepatogenih i patogenih bakterija in vitro. Inhibicijski učinak G-90 na rast bakterijskih kultura uvjetno-patogenih vrsta bio je za 21% jači u odnosu na nepatogene bakterija. Analizom vrijednosti CFU/mL, dobivenih iz obriska kožnih rana miševa, pokazano je da G-90 i in vivo u koncentracijama od 10 ng/mL i 10 μg/mL inhibicijski djeluje na rast bakterija u tretiranim ranama. U ovom radu opisana antibakterijska aktivnost G-90, uz ranije utvrđene biološke aktivnosti, upućuje da bi pripravak mogao biti od interesa za klinička istraživanja u veterinarskoj i humanoj medicini