Hyaluronate–Peanut Agglutinin Conjugates for Target-Specific Bioimaging of Colon Cancer

Colon cancer is one of the most common death-related cancers in the world. For treating colon cancer, it is crucial to detect and remove malignant lesions early. Here, we developed hyaluronate (HA)–peanut agglutinin (PNA) conjugates for the bioimaging of colon cancer. The HA–PNA conjugates were successfully synthesized by the coupling reaction between aldehyde-modified HA and the N-terminal amine group of PNA. For diagnostic imaging, rhodamine B (RhoB) was chemically conjugated onto PNA in HA–PNA conjugates. After intraluminal injection of HA–PNA–RhoB conjugates into tumor-bearing mice, small-sized colon cancers could be effectively visualized by ex vivo imaging with an in vivo imaging system (IVIS) and a two-photon microscope. With these results taken together, we could confirm the feasibility of HA–PNA–RhoB conjugates as a bioimaging agent for detecting colon cancers