11 research outputs found
Studies of Promoter Recognition and Start Site Selection by T7 RNA Polymerase Using a Comprehensive Collection of Promoter Variants â€
No full textThe Stability of Abortively Cycling T7 RNA Polymerase Complexes Depends upon Template Conformation â€
No full textA Transient Receptor Potential Channel Expressed in Taste Receptor Cells
No full textWe used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as -gustducin, G13, phospholipase C-2 (PLC-2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds
A Transient Receptor Potential Channel Expressed in Taste Receptor Cells
No full textWe used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as -gustducin, G13, phospholipase C-2 (PLC-2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds
Self-Assembly Behavior of Triphenylene-Based Side-Chain Discotic Liquid Crystalline Polymers
No full textWe constructed a generic coarse-grained
model of triphenylene-based
side-chain discotic liquid crystalline polymers (SDLCPs). Then dissipative
particle dynamics (DPD) simulation was employed to systematically
study how composition and structural factors of SDLCPs such as molecular
weight, main chain, spacer and aliphatic tails, and the incompatibility
between mesogenic core and its substituents influence their mesophases
and self-assembly behavior. Eight mesophases were obtained by changing
the factors mentioned above. The eight phases are hexagonal columnar–amorphous
(Col<sub>h</sub>-Am), nematic columnar–amorphous (Col<sub>ne</sub>-Am), nematic columnar–clustered (Col<sub>ne</sub>-Clu), nematic
columnar–columnar (Col<sub>ne</sub>-Col), random columns–amorphous
(Col<sub>ran</sub>-Am), random columnar–clustered (Col<sub>ran</sub>-Clu), amorphous–amorphous (Am-Am), and sphere–amorphous
(Sph-Am). The name of mesophase is denoted as “assembly of
discogens-aggregation of backbone”. By checking conformation
of molecules, the intracolumnar self-assembling patterns based on
the discrete columnar stack (DCS) were observed in Col<sub>h</sub>-Am, Col<sub>ne</sub>-Am, and Col<sub>ne</sub>-Clu, while Col<sub>ne</sub>-Col and Sph-Am adopted different packing modes. Moreover,
molecular weight effect and positive coupling of spacer were discolosed,
which matches well with experiments. Moderate or strong incompatibility
between mesogenic cores and substituents and proper peripheral aliphatic
tails are needed for SDLCPs to form ordered mesophases. The in-depth
understranding of their superstructures may offer inspiring guidance
for rational polymer design, preparation, and further exploration
in optoelectronic field
A mutant T7 RNA polymerase that is defective in RNA binding and blocked in the early stages of transcription
No full textA Transient Receptor Potential Channel Expressed in Taste Receptor Cells
No full textWe used differential screening of cDNAs from individual taste receptor cells to identify candidate taste transduction elements in mice. Among the differentially expressed clones, one encoded Trpm5, a member of the mammalian family of transient receptor potential (TRP) channels. We found Trpm5 to be expressed in a restricted manner, with particularly high levels in taste tissue. In taste cells, Trpm5 was coexpressed with taste-signaling molecules such as -gustducin, G13, phospholipase C-2 (PLC-2) and inositol 1,4,5-trisphosphate receptor type III (IP3R3). Our heterologous expression studies of Trpm5 indicate that it functions as a cationic channel that is gated when internal calcium stores are depleted. Trpm5 may be responsible for capacitative calcium entry in taste receptor cells that respond to bitter and/or sweet compounds
