Survival of mice infected with TIGR4 and Δ<i>idtr</i>.

<p>CBA/CaHN-Btk<i>^xid</i>/J mice were inoculated (A) intranasally with 10⁶ CFU and (B) intravenously with 10⁵ CFU of TIGR4 and Δ<i>idtr</i>. Kaplan Meier curves shown are a representative of triplicate experiments (n = 5 in each experiment).</p

Average bacterial counts from mouse blood TIGR4 and Δ<i>idtr</i>.

<p>A group of 5 mice each were infected intravenously with 10⁵ CFU of TIGR4 or Δ<i>idtr</i>. Blood samples at different time points were plated to determine bacterial counts. The error bars represent standard error of mean. ^**Significantly decreased as compared to TIGR4 infected blood counts (P<0.01).</p

Growth of TIGR4 and Δ<i>idtr</i> and Gram stain morphology of Δ<i>idtr</i> in vitro.

<p>The growth of TIGR4 and Δ<i>idtr</i> in CDM and iron depleted CDM was monitored by measuring absorbance at 600 nm. B) The morphology of Δ<i>idtr</i> was observed in (I) Iron depleted CDM (II) CDM by Gram staining. The results shown are average of three independent experiments cells grown in iron.</p

Pneumococcal gene expression in Δ<i>idtr</i> in vitro and in vivo.

<p>Expression of ten pneumococcal genes in Δ<i>idtr</i> relative to TIGR4 in CDM (A) and from nasopharyngeal washes, lung homogenates and blood samples (B) was quantified by RT-PCR. Each experiment was performed using three separate biological sample, each done in triplicate.</p

Schematic representation of Δ<i>idtr</i> construction.

<p>H-<i>HindIII</i>, B-<i>BamHI</i>. T1, T2 amplify the <i>tmp</i> cassette (495 bp); T1 and T2 have H and B at 5′ end. I1, I2 and I3, I4 amplify 5′ and 3′ end of <i>idtr</i>. I2 and I3 have H and B at 5′ end. I1, I2 amplify a 945 bp product and I3, I4 amplify a product of 489 bp.</p