5 research outputs found
Light-Triggered Intramolecular Cyclization in Poly(lactic-<i>co</i>-glycolic acid)-Based Polymers for Controlled Degradation
Polylactide (PLA) and polyÂ(dl-lactide-<i>co</i>-glycolide) (PLGA) are two
prominent FDA-approved polymers
because of their useful biodegradation into largely innocuous substances.
Their hydrolytic degradation is slow and offers minimal control over
degradation kinetics, especially in the minutes time scale. However,
molecular engineering of their structures could allow triggered degradation.
We have synthesized, by ring-opening polymerization (ROP), a series
of PLGA-based polymers containing pendant nucleophiles protected with
photocleavable groups. Upon deprotection, two of the polymers degrade
rapidly via intramolecular cyclization into small molecules. Nanoparticles
formulated from these polymers undergo rapid structural changes in
response to UV light. This work introduces a novel polymeric structure
to enable rapid on-demand degradation and expands the library of polymers
that degrade by cyclization
Iron Oxide Nanoparticle-Based Magnetic Resonance Method to Monitor Release Kinetics from Polymeric Particles with High Resolution
A new method to precisely monitor rapid release kinetics
from polymeric
particles using super paramagnetic iron oxide nanoparticles, specifically
by measuring spin–spin relaxation time (<i>T</i><sub>2</sub>), is reported. Previously, we have published the formulation
of logic gate particles from an acid-sensitive poly-β-aminoester
ketal-2 polymer. Here, a series of poly-β-aminoester ketal-2
polymers with varying hydrophobicities were synthesized and used to
formulate particles. We attempted to measure fluorescence of released
Nile red to determine whether the structural adjustments could finely
tune the release kinetics in the range of minutes to hours; however,
this standard technique did not differentiate each release rate of
our series. Thus, a new method based on encapsulation of iron oxide
nanoparticles was developed, which enabled us to resolve the release
kinetics of our particles. Moreover, the kinetics matched the relative
hydrophobicity order determined by octanol–water partition
coefficients. To the best of our knowledge, this method provides the
highest resolution of release kinetics to date
Long-Lasting and Efficient Tumor Imaging Using a High Relaxivity Polysaccharide Nanogel Magnetic Resonance Imaging Contrast Agent
Clinically
approved small-molecule magnetic resonance imaging (MRI)
contrast agents are all rapidly cleared from the body and offer weak
signal enhancement. To avoid repeated administration of contrast agent
and improve signal-to-noise ratios, agents with stronger signal enhancement
and better retention in tumors are needed. Therefore, we focused on
hydrogels because of their excellent water accessibility and biodegradability.
Gadolinium (Gd)-chelating cross-linkers were incorporated into self-assembled
pullulan nanogels to both impart magnetic properties and to stabilize
this material that has been extensively studied for medical applications.
We show that these Gd-chelating pullulan nanogels (Gd-CHPOA) have
the highest reported relaxivity for any hydrogel-based particles and
accumulate in the 4T1 tumors in mice at high levels 4 h after injection.
This combination offers high signal enhancement and lasts up to 7
days to delineate the tumor clearly for longer imaging time scales.
Importantly, this long-term accumulation does not cause any damage
or toxicity in major organs up to three months after injection. Our
work highlights the clinical potential of Gd-CHPOA as a tumor-imaging
MRI contrast agent, permitting tumor identification and assessment
with a high signal-to-background ratio
Collective Activation of MRI Agents via Encapsulation and Disease-Triggered Release
An activation mechanism based on
encapsulated ultrasmall gadolinium
oxide nanoparticles (Gd oxide NPs) in bioresponsive polymer capsules
capable of triggered release in response to chemical markers of disease
(i.e., acidic pH, H<sub>2</sub>O<sub>2</sub>) is presented. Inside
the hydrophobic polymeric matrices, the Gd oxide NPs are shielded
from the aqueous environment, silencing their ability to enhance water
proton relaxation. Upon disassembly of the polymeric particles, activation
of multiple contrast agents generates a strong positive contrast enhancement
of >1 order of magnitude
Short Soluble Coumarin Crosslinkers for Light-Controlled Release of Cells and Proteins from Hydrogels
Materials
that degrade or dissociate in response to low power light
promise to enable on-demand, precisely localized delivery of drugs
or bioactive molecules in living systems. Such applications remain
elusive because few materials respond to wavelengths that appreciably
penetrate tissues. The photocage bromohydroxycoumarin (Bhc) is efficiently
cleaved upon low-power ultraviolet (UV) and near-infrared (NIR) irradiation
through one- or two-photon excitation, respectively. We have designed
and synthesized a short Bhc-bearing crosslinker to create light-degradable
hydrogels and nanogels. Our crosslinker breaks by intramolecular cyclization
in a manner inspired by the naturally occurring ornithine lactamization,
in response to UV and NIR light, enabling rapid degradation of polyacrylamide
gels and release of small hydrophilic payloads such as an ∼10
nm model protein and murine mesenchymal stem cells, with no background
leakage