Comparative transcriptome profiling of the fertile and sterile flower buds of a dominant genic male sterile line in sesame (Sesamum indicum L.)

Expressions and annotations of the 1502 differentially expressed unigenes in sesame. (XLSX 338 kb

Effect of Pathogenic Fungal Infestation on the Berry Quality and Volatile Organic Compounds of Cabernet Sauvignon and Petit Manseng Grapes

The effect of pathogenic fungal infestation on berry quality and volatile organic compounds (VOCs) of Cabernet Sauvignon (CS) and Petit Manseng (PM) were investigated by using biochemical assays and gas chromatography-ion mobility spectrometry. No significant difference in diseases-affected grapes for 100-berry weight. The content of tannins and vitamin C decreased significantly in disease-affected grapes, mostly in white rot-affected PM, which decreased by 71.67% and 66.29%. The reduced total flavonoid content in diseases-affected grape, among which the least and most were anthracnose-affected PM (1.61%) and white rot-affected CS (44.74%). All diseases-affected CS had much higher titratable acid, a maximum (18.86 g/100 ml) was observed in the gray mold-affected grapes, while only anthracnose-affected grapes with a higher titratable acid level (21.8 g/100 mL) were observed in PM. A total of 61 VOCs were identified, including 14 alcohols, 13 esters, 12 aldehydes, 4 acids, 4 ketones, 1 ether, and 13 unknown compounds, which were discussed from different functional groups, such as C6-VOCs, alcohols, ester acetates, aldehydes, and acids. The VOCs of CS changed more than that of Petit Manseng’s after infection, while gray mold-affected Cabernet Sauvignon had the most change. C6-VOCs, including hexanal and (E)-2-hexenal were decreased in all affected grapes. Some unique VOCs may serve as hypothetical biomarkers to help us identify specific varieties of pathogenic fungal infestation

Citrus sinensis MYB Transcription Factor CsMYB85 Induce Fruit Juice Sac Lignification Through Interaction With Other CsMYB Transcription Factors

Varieties of Citrus are commercially important fruits that are cultivated worldwide and are valued for being highly nutritious and having an appealing flavor. Lignification of citrus fruit juice sacs is a serious physiological disorder that occurs during postharvest storage, for which the underlying transcriptional regulatory mechanisms remain unclear. In this study, we identified and isolated a candidate MYB transcription factor, CsMYB85, that is involved in the regulation of lignin biosynthesis in Citrus sinensis, which has homologs in Arabidopsis and other plants. We found that during juice sac lignification, CsMYB85 expression levels increase significantly, and therefore, suspected that this gene may control lignin biosynthesis during the lignification process. Our results indicated that CsMYB85 binds the CsMYB330 promoter, regulates its expression, and interacts with CsMYB308 in transgenic yeast and tobacco. A transient expression assay indicated that Cs4CL1 expression levels and lignin content significantly increased in fruit juice sacs overexpressing CsMYB85. At4CL1 expression levels and lignin content were also significantly increased in Arabidopsis overexpressing CsMYB85. We accordingly present convincing evidence for the participation of the CsMYB85 transcription factor in fruit juice sac lignification, and thereby provide new insights into the transcriptional regulation of this process in citrus fruits

Epidermal Growth Factor Gene Polymorphism and Risk of Hepatocellular Carcinoma: A Meta-Analysis

BACKGROUND: Hepatocarcinogenesis is a complex process that may be influenced by many factors, including polymorphism in the epidermal growth factor (EGF) gene. Previous work suggests an association between the EGF 61*A/G polymorphism (rs4444903) and susceptibility to hepatocellular carcinoma (HCC), but the results have been inconsistent. Therefore, we performed a meta-analysis of several studies covering a large population to address this controversy. METHODS: PubMed, EMBASE, Google Scholar and the Chinese National Knowledge Infrastructure databases were systematically searched to identify relevant studies. Data were abstracted independently by two reviewers. A meta-analysis was performed to examine the association between EGF 61*A/G polymorphism and susceptibility to HCC. Odds ratios (ORs) and 95% confidence intervals (95% CIs) were calculated. RESULTS: Eight studies were chosen in this meta-analysis, involving 1,304 HCC cases (1135 Chinese, 44 Caucasian and 125 mixed) and 2,613 controls (1638 Chinese, 77 Caucasian and 898 mixed). The EGF 61*G allele was significantly associated with increased risk of HCC based on allelic contrast (OR = 1.29, 95% CI = 1.16-1.44, p<0.001), homozygote comparison (OR = 1.79, 95% CI = 1.39-2.29, p<0.001) and a recessive genetic model (OR = 1.34, 95% CI = 1.16-1.54, p<0.001), while patients carrying the EGF 61*A/A genotype had significantly lower risk of HCC than those with the G/A or G/G genotype (A/A vs. G/A+G/G, OR = 0.66, 95% CI = 0.53-0.83, p<0.001). CONCLUSION: The 61*G polymorphism in EGF is a risk factor for hepatocarcinogenesis while the EGF 61*A allele is a protective factor. Further large and well-designed studies are needed to confirm this conclusion

Habenula “Cholinergic” Neurons Corelease Glutamate and Acetylcholine and Activate Postsynaptic Neurons via Distinct Transmission Modes

Acetylcholine is an important neurotransmitter, and the habenulo-interpeduncular projection is a major cholinergic pathway in the brain. To study the physiological properties of cholinergic transmission in the interpeduncular nucleus (IPN), we used a transgenic mouse line in which the light-gated cation channel ChannelRhodopsin-2 is selectively expressed in cholinergic neurons. Cholinergic axonal terminals were activated by light pulses, and postsynaptic responses were recorded from IPN neurons. Surprisingly, brief photostimulation produces fast excitatory postsynaptic currents that are mediated by ionotropic glutamate receptors, suggesting wired transmission of glutamate. By contrast, tetanic photostimulation generates slow inward currents that are largely mediated by nicotinic acetylcholine receptors, suggesting volume transmission of acetylcholine. Finally, vesicular transporters for glutamate and acetylcholine are coexpressed on the same axonal terminals in the IPN. These results strongly suggest that adult brain “cholinergic” neurons can corelease glutamate and acetylcholine, but these two neurotransmitters activate postsynaptic neurons via different transmission modes

Graphene-metal nanoparticles for enhancing thermoelectric power factor

Chemical vapor deposition (CVD) grown graphene has been reported for a myriad of nanoelectronics and nanophotonics applications. However, CVD grown graphene suffers from low electrical conductivity due to the presence of multiple grain boundaries and intragranular defects. In order to improve its performance, graphene is often functionalized with other materials. In this work, graphene was hybridized with metallic nanoparticles (Au, Ag and Pt). Metal-containing inorganic compound solution was drop casted onto patterned graphene and was subsequently reduced by ultraviolet light. The hybridized graphene systems were explored as possible thermoelectric generators. It was shown that nanoparticles hybridized graphene displayed a significant decrease in sheet resistance. Amongst them, Au-graphene revealed an 80% decrease in sheet resistance. Ag- and Pt-graphene did not show any enhancement in the Seebeck effect while Au-graphene observed a 25% decline due to the thermal cooling effect. Overall, thermoelectric power factor was shown to increase by a factor of 2.96, 1.72 and 1.25 times for Au-graphene, Ag-graphene, and Pt-graphene, respectively.Ministry of Education (MOE)This work was supported by the Ministry of Education, Singapore under Grant MOE2015-T2-2-043. The review of this letter was arranged by Associate Editor G.-B. Lee

The application of multi-baseline digital close-range photogrammetry in three-dimensional imaging and measurement of dental casts

<div><p>Objective</p><p>To explore a new technique for reconstructing and measuring three-dimensional (3D) models of orthodontic plaster casts using multi-baseline digital close-range photogrammetry (MBDCRP) with a single-lens reflex camera.</p><p>Study design</p><p>Thirty sets of orthodontic plaster casts that do not exhibit severe horizontal overlap (>2 mm) between any two teeth were recorded by a single-lens reflex camera with 72 pictures taken in different directions. The 3D models of these casts were reconstructed and measured using the open source software MeshLab. These parameters, including mesio-distal crown diameter, arch width, and arch perimeter, were recorded six times on both the 3D digital models and on plaster casts by two examiners. Statistical analysis was carried out using the Bland–Altman method <a href="http://www.sciencedirect.com/science/article/pii/S0736467908006008" target="_blank">to measure agreement between the novel method and the traditional calliper method</a> by calculating the differences between mean values.</p><p>Results</p><p>The average differences between the measurements of the photogrammetric 3D models and the plaster casts were 0.011–0.402mm. The mean differences between measurements obtained by the photogrammetric 3D models and the dental casts were not significant except for the lower arch perimeter (<i>P</i>>0.05), and all the differences were regarded as clinically acceptable (<0.5 mm).</p><p>Conclusions</p><p>Measurements obtained by MBDCRP are compared well with those obtained from plaster casts, indicating that MBDCRP is an alternate way to store and measure dental plaster casts without severe horizontal overlap between any two teeth.</p></div

Measurements of intercanine and intermolar width using MeshLab.

<p>Measurements of intercanine and intermolar width using MeshLab.</p