3 research outputs found
Role of an Active Site Loop in the Promiscuous Activities of <i>Amycolatopsis</i> sp. T‑1-60 NSAR/OSBS
The <i>o</i>-succinylbenzoate synthase (OSBS) family
is part of the functionally diverse enolase superfamily. Many proteins
in one branch of the OSBS family catalyze both OSBS and <i>N</i>-succinylamino acid racemization in the same active site. In some
promiscuous NSAR/OSBS enzymes, NSAR activity is biologically significant
in addition to or instead of OSBS activity. Identifying important
residues for each reaction could provide insight into how proteins
evolve new functions. We have made a series of mutations in <i>Amycolatopsis</i> sp. T-1-60 NSAR/OSBS in an active site loop,
referred to as the 20s loop. This loop affects substrate specificity
in many members of the enolase superfamily but is poorly conserved
within the OSBS family. Deletion of this loop decreased OSBS and NSAR
catalytic efficiency by 4500-fold and 25,000-fold, respectively, showing
that it is essential. Most point mutations had small effects, changing
the efficiency of both NSAR and OSBS activities <10-fold compared
to that of the wild type. An exception was F19A, which reduced <i>k</i><sub>cat</sub>/<i>K</i><sub>M</sub><sup>OSBS</sup> 200-fold and <i>k</i><sub>cat</sub>/<i>K</i><sub>M</sub><sup>NSAR</sup> 120-fold. Mutating the surface residue
R20E, which can form a salt bridge to help close the 20s loop over
the active site, had a more modest effect, decreasing <i>k</i><sub>cat</sub>/<i>K</i><sub>M</sub> of OSBS and NSAR reactions
32- and 8-fold, respectively. Several mutations increased <i>K</i><sub>M</sub> of the NSAR reaction more than that of the
OSBS reaction. Thus, both activities require the 20s loop, but differences
in how mutations affect OSBS and NSAR activities suggest that some
substitutions in this loop made a small contribution to the evolution
of NSAR activity, although additional mutations were probably required
Comparison of <i>Alicyclobacillus acidocaldarius</i> <i>o</i>‑Succinylbenzoate Synthase to Its Promiscuous <i>N</i>‑Succinylamino Acid Racemase/<i>o</i>‑Succinylbenzoate Synthase Relatives
Studying
the evolution of catalytically promiscuous enzymes like
those from the <i>N</i>-succinylamino acid racemase/<i>o</i>-succinylbenzoate synthase (NSAR/OSBS) subfamily can reveal
mechanisms by which new functions evolve. Some enzymes in this subfamily
have only OSBS activity, while others catalyze OSBS and NSAR reactions.
We characterized several NSAR/OSBS subfamily enzymes as a step toward
determining the structural basis for evolving NSAR activity. Three
enzymes were promiscuous, like most other characterized NSAR/OSBS
subfamily enzymes. However, <i>Alicyclobacillus acidocaldarius</i> OSBS (AaOSBS) efficiently catalyzes OSBS activity but lacks detectable
NSAR activity. Competitive inhibition and molecular modeling show
that AaOSBS binds <i>N</i>-succinylphenylglycine with moderate
affinity in a site that overlaps its normal substrate. On the basis
of possible steric conflicts identified by molecular modeling and
sequence conservation within the NSAR/OSBS subfamily, we identified
one mutation, Y299I, that increased NSAR activity from undetectable
to 1.2 × 10<sup>2</sup> M<sup>–1</sup> s<sup>–1</sup> without affecting OSBS activity. This mutation does not appear to
affect binding affinity but instead affects <i>k</i><sub>cat</sub>, by reorienting the substrate or modifying conformational
changes to allow both catalytic lysines to access the proton that
is moved during the reaction. This is the first site known to affect
reaction specificity in the NSAR/OSBS subfamily. However, this gain
of activity was obliterated by a second mutation, M18F. Epistatic
interference by M18F was unexpected because a phenylalanine at this
position is important in another NSAR/OSBS enzyme. Together, modest
NSAR activity of Y299I AaOSBS and epistasis between sites 18 and 299
indicate that additional sites influenced the evolution of NSAR reaction
specificity in the NSAR/OSBS subfamily