High-Performance Strain Sensors Based on Spirally Structured Composites with Carbon Black, Chitin Nanocrystals, and Natural Rubber

In this research, a new type of conductive composite with high tensile strength, high elasticity, and cost competitiveness has been developed through solution mixing–spraying–rolling methods. Naturel rubber (NR) latex with chitin nanocrystals (ChNCs) as reinforcing filler and carbon black (CB) are thermally sprayed on glass substrate layer by layer, and then, spirally structured conductive composites are obtained by rolling the sheets. When the CB content is 4.44%, the conductivity of the NR/ChNCs-CB composite can reach 6.92 s/m. The tensile strength of 5% ChNCs reinforced conductive composites is 3.47 MPa, which is 3.1 times that of NR-CB composites without ChNCs. The strain sensor exhibits a high gauge factor (GF ≈ 5) and electrical conductivity stability in a small deformation range and still shows good stability and recoverability upon 25%, 50%, and 100% strain. The high-sensitivity strain sensors are further employed for monitoring human activities such as finger movements and pronunciation, which shows good reproducibility and reliability. This study provides a routine of preparing highly stretchable and multifunctional strain sensors based on inexpensive raw materials by a simple manner, which opens up new opportunities for the development of stretchable electronic devices

High-Performance Strain Sensors Based on Spirally Structured Composites with Carbon Black, Chitin Nanocrystals, and Natural Rubber

In this research, a new type of conductive composite with high tensile strength, high elasticity, and cost competitiveness has been developed through solution mixing–spraying–rolling methods. Naturel rubber (NR) latex with chitin nanocrystals (ChNCs) as reinforcing filler and carbon black (CB) are thermally sprayed on glass substrate layer by layer, and then, spirally structured conductive composites are obtained by rolling the sheets. When the CB content is 4.44%, the conductivity of the NR/ChNCs-CB composite can reach 6.92 s/m. The tensile strength of 5% ChNCs reinforced conductive composites is 3.47 MPa, which is 3.1 times that of NR-CB composites without ChNCs. The strain sensor exhibits a high gauge factor (GF ≈ 5) and electrical conductivity stability in a small deformation range and still shows good stability and recoverability upon 25%, 50%, and 100% strain. The high-sensitivity strain sensors are further employed for monitoring human activities such as finger movements and pronunciation, which shows good reproducibility and reliability. This study provides a routine of preparing highly stretchable and multifunctional strain sensors based on inexpensive raw materials by a simple manner, which opens up new opportunities for the development of stretchable electronic devices

Sustainable, High-Performance, and Biodegradable Plastics Made from Chitin

A high-performance biodegradable plastic was made from a chitin KOH/urea solution. The solution was transferred into a hydrogel by cross-linking using epichlorohydrin and ethanol immersion, and a chitin bioplastic was finally prepared by drying in a mold at 40 °C. The solution concentration positively impacts viscosity, crystallinity, and smoothness. A 4% chitin bioplastic exhibits high barrier properties, flame retardancy, high-temperature resistance, mechanical properties (tensile strength up to 107.1 MPa), and soil degradation properties. The chitin bioplastic can be completely degraded by microorganisms in 7 weeks. In addition, biosafety tests suggest that chitin is safe for cells and crops (wheat and mung beans). The chitin bioplastic was further applied to containers, straws, cups, and photoprotection, and it was found that the water resistance and transparency were comparable to those of commercial polypropylene plastics. Due to the excellent performance, safety, and sustainability of the chitin bioplastic, it is expected to become a good substitute for conventional fossil fuel-based plastics

Sustainable, High-Performance, and Biodegradable Plastics Made from Chitin

A high-performance biodegradable plastic was made from a chitin KOH/urea solution. The solution was transferred into a hydrogel by cross-linking using epichlorohydrin and ethanol immersion, and a chitin bioplastic was finally prepared by drying in a mold at 40 °C. The solution concentration positively impacts viscosity, crystallinity, and smoothness. A 4% chitin bioplastic exhibits high barrier properties, flame retardancy, high-temperature resistance, mechanical properties (tensile strength up to 107.1 MPa), and soil degradation properties. The chitin bioplastic can be completely degraded by microorganisms in 7 weeks. In addition, biosafety tests suggest that chitin is safe for cells and crops (wheat and mung beans). The chitin bioplastic was further applied to containers, straws, cups, and photoprotection, and it was found that the water resistance and transparency were comparable to those of commercial polypropylene plastics. Due to the excellent performance, safety, and sustainability of the chitin bioplastic, it is expected to become a good substitute for conventional fossil fuel-based plastics

Sustainable, High-Performance, and Biodegradable Plastics Made from Chitin

A high-performance biodegradable plastic was made from a chitin KOH/urea solution. The solution was transferred into a hydrogel by cross-linking using epichlorohydrin and ethanol immersion, and a chitin bioplastic was finally prepared by drying in a mold at 40 °C. The solution concentration positively impacts viscosity, crystallinity, and smoothness. A 4% chitin bioplastic exhibits high barrier properties, flame retardancy, high-temperature resistance, mechanical properties (tensile strength up to 107.1 MPa), and soil degradation properties. The chitin bioplastic can be completely degraded by microorganisms in 7 weeks. In addition, biosafety tests suggest that chitin is safe for cells and crops (wheat and mung beans). The chitin bioplastic was further applied to containers, straws, cups, and photoprotection, and it was found that the water resistance and transparency were comparable to those of commercial polypropylene plastics. Due to the excellent performance, safety, and sustainability of the chitin bioplastic, it is expected to become a good substitute for conventional fossil fuel-based plastics

Stripe-like Clay Nanotubes Patterns in Glass Capillary Tubes for Capture of Tumor Cells

Here, we used capillary tubes to evaporate an aqueous dispersion of halloysite nanotubes (HNTs) in a controlled manner to prepare a patterned surface with ordered alignment of the nanotubes . Sodium polystyrenesulfonate (PSS) was added to improve the surface charges of the tubes. An increased negative charge of HNTs is realized by PSS coating (from −26.1 mV to −52.2 mV). When the HNTs aqueous dispersion concentration is higher than 10%, liquid crystal phenomenon of the dispersion is found. A typical shear flow behavior and decreased viscosity upon shear is found when HNTs dispersions with concentrations higher than 10%. Upon drying the HNTs aqueous dispersion in capillary tubes, a regular pattern is formed in the wall of the tube. The width and spacing of the bands increase with HNTs dispersion concentration and decrease with the drying temperature for a given initial concentration. Morphology results show that an ordered alignment of HNTs is found especially for the sample of 10%. The patterned surface can be used as a model for preparing PDMS molding with regular micro-/nanostructure. Also, the HNTs rough surfaces can provide much higher tumor cell capture efficiency compared to blank glass surfaces. The HNTs ordered surfaces provide promising application for biomedical areas such as biosensors

Self-Assembling Halloysite Nanotubes into Concentric Ring Patterns in a Sphere-on-Flat Geometry

Highly ordered and concentric ring patterns consisting of halloysite nanotubes (HNTs) with hierarchical cholesteric architectures are prepared by evaporation-induced self-assembly in a sphere-on-flat geometry. The structure and properties of HNTs are investigated. HNTs show a perfect tubular morphology on the nanoscale with high dispersion stability in water. Upon drying the HNTs aqueous suspension in a sphere-on-flat confined space, regular concentric HNTs rings are formed on the substrate via a self-assembly process. The widths of the inner and outer rings and the spacing between the adjacent rings increase with an increase in the concentration of the HNTs suspension. The highly ordered and concentric HNTs rings show a pronounced Maltese cross-like pattern under crossed polarizers, which suggests the formation of hierarchical cholesteric architectures. Scanning electron microscopy and atomic force microscopy observations show a disclination alignment of HNTs in the ring strips, especially with a high concentration of the HNTs suspension. The patterned rough surfaces of the HNTs show low cytotoxicity and can be used as a cell-supporting scaffold. The HNTs rings can guide the growth and orientation of C2C12 myoblast cells perpendicular to the rings. This work provides a simple, repeatable, mild, and high-efficiency method for obtaining HNTs with hierarchical architectures, which show potential for a large variety of applications, for example, in vascular grafts and skin regeneration

Functionalization of Halloysite Nanotubes via Grafting of Dendrimer for Efficient Intracellular Delivery of siRNA

Here, polyamidoamine grafted halloysite nanotubes (PAMAM-<i>g</i>-HNTs) were synthesized for loading of siRNA in order to intracellular delivery of siRNA and treat of breast cancer via gene therapy. The successful grafting of PAMAM on HNTs was confirmed by various analytical methods. The size, zeta potential, and grafting ratio of PAMAM-<i>g</i>-HNTs is ∼206.2 nm, +19.8 mV, and 3.04%, respectively. PAMAM-<i>g</i>-HNTs showed good cytocompatibility toward HUVECs (84.7%) and MCF-7 cells (82.3%) even at high concentration of 100 μg/mL. PAMAM-<i>g</i>-HNTs/siRNA exhibited enhanced cellular uptake efficiency of 94.3% compared with Lipofectamine 2000 (Lipo2000)/siRNA (83.6%). PAMAM-<i>g</i>-HNTs/small interfering RNA-vascular endothelial growth factor (siVEGF) led to 78.0% knockdown of cellular VEGF mRNA and induced 33.6% apoptosis in the MCF-7 cells, which is also much higher than that of Lipo2000/siVEGF. In vivo anti-cancer results demonstrated that PAMAM-<i>g</i>-HNTs/siVEGF treated 4T1-bearing mice showed enhanced anti-cancer efficacy than Lipo2000/siVEGF group. Also, the nanocarrier system showed negligible toxic effects toward the major organs of mice. In vivo fluorescence imaging studies showed that there is a slight decrease in the fluorescence signal of PAMAM-<i>g</i>-HNTs/cy5-siVEGF after 72 h post-injection. Therefore, PAMAM-<i>g</i>-HNTs show promising application as novel nanovectors for siRNA delivery and gene therapy of cancer

3D Printing Drug-Free Scaffold with Triple-Effect Combination Induced by Copper-Doped Layered Double Hydroxides for the Treatment of Bone Defects

Tissue-engineered poly­(l-lactide) (PLLA) scaffolds have been widely used to treat bone defects; however, poor biological activities have always been key challenges for its further application. To address this issue, introducing bioactive drugs or factors is the most commonly used method, but there are often many problems such as high cost, uncontrollable and monotonous drug activity, and poor bioavailability. Here, a drug-free 3D printing PLLA scaffold with a triple-effect combination induced by surface-modified copper-doped layered double hydroxides (Cu-LDHs) is proposed. In the early stage of scaffold implantation, Cu-LDHs exert a photothermal therapy (PTT) effect to generate high temperature to effectively prevent bacterial infection. In the later stage, Cu-LDHs can further have a mild hyperthermia (MHT) effect to stimulate angiogenesis and osteogenic differentiation, demonstrating excellent vascularization and osteogenic activity. More importantly, with the degradation of Cu-LDHs, the released Cu2+ and Mg2+ provide an ion microenvironment effect and further synergize with the MHT effect to stimulate angiogenesis and osteogenic differentiation, thus more effectively promoting the healing of bone tissue. This triple-effect combined scaffold exhibits outstanding antibacterial, osteogenic, and angiogenic activities, as well as the advantages of low cost, convenient procedure, and long-term efficacy, and is expected to provide a promising strategy for clinical repair of bone defects

Bone ECM-like 3D Printing Scaffold with Liquid Crystalline and Viscoelastic Microenvironment for Bone Regeneration

Implanting a 3D printing scaffold is an effective therapeutic strategy for personalized bone repair. As the key factor for the success of bone tissue engineering, the scaffold should provide an appropriate bone regeneration microenvironment and excellent mechanical properties. In fact, the most ideal osteogenic microenvironment is undoubtedly provided by natural bone extracellular matrix (ECM), which exhibits liquid crystalline and viscoelastic characteristics. However, mimicking a bone ECM-like microenvironment in a 3D structure with outstanding mechanical properties is a huge challenge. Herein, we develop a facile approach to fabricate a bionic scaffold perfectly combining bone ECM-like microenvironment and robust mechanical properties. Creatively, 3D printing a poly(l-lactide) (PLLA) scaffold was effectively strengthened via layer-by-layer electrostatic self-assembly of chitin whiskers. More importantly, a kind of chitin whisker/chitosan composite hydrogel with bone ECM-like liquid crystalline state and viscoelasticity was infused into the robust PLLA scaffold to build the bone ECM-like microenvironment in 3D structure, thus highly promoting bone regeneration. Moreover, deferoxamine, an angiogenic factor, was encapsulated in the composite hydrogel and sustainably released, playing a long-term role in angiogenesis and thereby further promoting osteogenesis. This scaffold with bone ECM-like microenvironment and excellent mechanical properties can be considered as an effective implantation for bone repair