2 research outputs found
Development of Photoaffinity Probe for the Discovery of Steviol Glycosides Biosynthesis Pathway in <i>Stevia rebuadiana</i> and Rapid Substrate Screening
Functional discovery
and characterization of the target enzymes
responsible for the biosynthesis pathway coded for the genes is ongoing,
and the unknown functional diversity of this class of enzymes has
been revealed by genome sequencing. Commonly, it is feasible in annotating
of biosynthetic genes of prokaryotes due to the existence of gene
clusters of secondary metabolites. However, in eukaryotes, the biosynthetic
genes are not compactly clustered in the way of prokaryotes. Hence,
it remains challenging to identify the biosynthetic pathways of newly
discovered natural products in plants. Steviol glycosides are one
class of natural sweeteners found in high abundance in the herb <i>Stevia rebaudiana</i>. Here, we applied the chemoproteomic strategy
for the proteomic profiling of the biosynthetic enzymes of steviol
glycosides in <i>Stevia rebaudiana</i>. We not only identified
a steviol-catalyzing UDP-glycosyltransferase (UGT) UGT73E1 involved
in steviol glycoside biosynthesis but also built up a probe-based
platform for the screening of potential substrates of functional uncharacterized
UGT rapidly. This approach would be a complementary tool in mining
novel synthetic parts for assembling of synthetic biological systems
for the biosynthesis of other complex natural products
Development of Photoaffinity Probe for the Discovery of Steviol Glycosides Biosynthesis Pathway in <i>Stevia rebuadiana</i> and Rapid Substrate Screening
Functional discovery
and characterization of the target enzymes
responsible for the biosynthesis pathway coded for the genes is ongoing,
and the unknown functional diversity of this class of enzymes has
been revealed by genome sequencing. Commonly, it is feasible in annotating
of biosynthetic genes of prokaryotes due to the existence of gene
clusters of secondary metabolites. However, in eukaryotes, the biosynthetic
genes are not compactly clustered in the way of prokaryotes. Hence,
it remains challenging to identify the biosynthetic pathways of newly
discovered natural products in plants. Steviol glycosides are one
class of natural sweeteners found in high abundance in the herb <i>Stevia rebaudiana</i>. Here, we applied the chemoproteomic strategy
for the proteomic profiling of the biosynthetic enzymes of steviol
glycosides in <i>Stevia rebaudiana</i>. We not only identified
a steviol-catalyzing UDP-glycosyltransferase (UGT) UGT73E1 involved
in steviol glycoside biosynthesis but also built up a probe-based
platform for the screening of potential substrates of functional uncharacterized
UGT rapidly. This approach would be a complementary tool in mining
novel synthetic parts for assembling of synthetic biological systems
for the biosynthesis of other complex natural products