Additional file 1: Table S1. of Reduced Kidney Function, Albuminuria, and Risks for All-cause and Cardiovascular Mortality in China: A Population-based Cohort Study

and S2.Hazard ratios for all-cause and cardiovascular mortality by indicators of chronic kidney disease among participants aged less than 65 years old, as well as among those aged more than 65 years old. (DOCX 17 kb

Table_1_Phytohormone biosynthesis and transcriptional analyses provide insight into the main growth stage of male and female cones Pinus koraiensis.xlsx

The cone is a crucial component of the whole life cycle of gymnosperm and an organ for sexual reproduction of gymnosperms. In Pinus koraiensis, the quantity and development process of male and female cones directly influence seed production, which in turn influences the tree’s economic value. There are, however, due to the lack of genetic information and genomic data, the morphological development and molecular mechanism of female and male cones of P. koraiensis have not been analyzed. Long-term phenological observations were used in this study to document the main process of the growth of both male and female cones. Transcriptome sequencing and endogenous hormone levels at three critical developmental stages were then analyzed to identify the regulatory networks that control these stages of cones development. The most significant plant hormones influencing male and female cones growth were discovered to be gibberellin and brassinosteroids, according to measurements of endogenous hormone content. Additionally, transcriptome sequencing allowed the identification of 71,097 and 31,195 DEGs in male and female cones. The synthesis and control of plant hormones during cones growth were discovered via enrichment analysis of key enrichment pathways. FT and other flowering-related genes were discovered in the coexpression network of flower growth development, which contributed to the growth development of male and female cones of P. koraiensis. The findings of this work offer a cutting-edge foundation for understanding reproductive biology and the molecular mechanisms that control the growth development of male and female cones in P. koraiensis.</p

DataSheet_1_Phytohormone biosynthesis and transcriptional analyses provide insight into the main growth stage of male and female cones Pinus koraiensis.docx

The cone is a crucial component of the whole life cycle of gymnosperm and an organ for sexual reproduction of gymnosperms. In Pinus koraiensis, the quantity and development process of male and female cones directly influence seed production, which in turn influences the tree’s economic value. There are, however, due to the lack of genetic information and genomic data, the morphological development and molecular mechanism of female and male cones of P. koraiensis have not been analyzed. Long-term phenological observations were used in this study to document the main process of the growth of both male and female cones. Transcriptome sequencing and endogenous hormone levels at three critical developmental stages were then analyzed to identify the regulatory networks that control these stages of cones development. The most significant plant hormones influencing male and female cones growth were discovered to be gibberellin and brassinosteroids, according to measurements of endogenous hormone content. Additionally, transcriptome sequencing allowed the identification of 71,097 and 31,195 DEGs in male and female cones. The synthesis and control of plant hormones during cones growth were discovered via enrichment analysis of key enrichment pathways. FT and other flowering-related genes were discovered in the coexpression network of flower growth development, which contributed to the growth development of male and female cones of P. koraiensis. The findings of this work offer a cutting-edge foundation for understanding reproductive biology and the molecular mechanisms that control the growth development of male and female cones in P. koraiensis.</p

Comparison of plasma suPAR levels between subgroups of variable clinical or histologic parameters in patients with IgAN.

<p>Abbreviations: IgAN, IgA nephropathy; CKD, chronic kidney disease.</p><p>Comparison of plasma suPAR levels between subgroups of variable clinical or histologic parameters in patients with IgAN.</p

Classification of canine oocytes.

<p>Oocytes were categorized as immature, mature, and aged.</p

Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.

<p>Canine cloned using <i>in vivo</i> matured oocytes as predicted by RIA and ECLI methods.</p

Comparison of progesterone concentrations (ng/mL) based on RIA and ECLI methods.

<p>(a'–j') Samples were separated based on the RIA data. Colocalization of RIA and ECLI methods were evaluated using the Pearson correlation coefficient. B) Average of RIA and ECLI methods. Data are presented as mean ± SD. The asterisk denotes significant differences (P<0.05).</p

Percentage of oocyte donors at each stage detected by (A) RIA or (B) ECLI methods using P4 concentrations of 4–8 ng/mL or (C) 6–15 ng/mL as ovulation standards.

<p>Pre: preovulation, IM, immature, M, mature and A, aging. Thirty dogs were used for analysis in each group.</p

Two canines derived from SCNT.

<p>(A) Canine derived using RIA and (B) ECLI methods.</p

Plasma suPAR levels among IgAN patients with different degrees of effacement of foot processes.

<p>Epithelial cells were detected by electron microscopy and the numbers of patients with segmental, most, and extensive effacement of foot processes were 408, 96, and 42, respectively. We found that plasma suPAR levels in the extensive effacement group were significantly higher than those in the segmental effacement group (2741.57 ± 894.81 pg/mL vs. 2407.68 ± 987.88 pg/mL, <i>P</i> = 0.036, Fig 3). Moreover, eGFR was comparable between groups (81.29 ± 30.19 mL/min/1.73 m² vs. 86.80 ± 26.60 mL/min/1.73 m², <i>P</i> = 0.208).</p