2 research outputs found
Structures of chitosan-based adhesive from Environment-friendly wood fibre composite with high bonding strength and water resistance
Synthesis mechanism and structure of the chitosan-based adhesiv
Zipper-Confined DNA Nanoframe for High-Efficient and High-Contrast Imaging of Heterogeneous Tumor Cell
Current
study in the heterogeneity and physiological
behavior of
tumor cells is limited by the fluorescence in situ hybridization technology in terms of probe assembly efficiency,
background suppression capability, and target compatibility. In a
typically well-designed assay, hybridization probes are constructed
in a confined nanostructure to achieve a rapid assembly for efficient
signal response, while the excessively high local concentration between
different probes inevitably leads to nonspecific background leakage.
Inspired by the fabric zipper, we propose a novel confinement reaction
pattern in a zipper-confined DNA nanoframe (ZCDN), where two kinds
of hairpin probes are independently anchored respective tracks. The
metastable states of the dual tracks can well avoid signal leakage
caused by the nonspecific probe configuration change. Biomarker-mediated
proximity ligation reduces the local distance of dual tracks, kinetically
triggering an efficient allosteric chain reaction between the hairpin
probes. This method circumvents nonspecific background leakage while
maintaining a high efficiency in responding to targets. ZCDN is employed
to track different cancer biomarkers located in both the cytoplasm
and cytomembrane, of which the expression level and oligomerization
behavior can provide crucial information regarding intratumoral heterogeneity.
ZCDN exhibits high target response efficiency and strong background
suppression capabilities and is compatible with various types of biological
targets, thus providing a desirable tool for advanced molecular diagnostics