Aberrant Cerebral Activity in Early Postmenopausal Women: A Resting-State Functional Magnetic Resonance Imaging Study

Background: Early postmenopausal women frequently suffer from cognitive impairments and emotional disorders, such as lack of attention, poor memory, deficits in executive function and depression. However, the underlying mechanisms of these impairments remain unclear.Method: Forty-three early postmenopausal women and forty-four age-matched premenopausal controls underwent serum sex hormone analysis, neuropsychological testing and resting-state functional magnetic resonance imaging (rs-fMRI). Degree centrality (DC) analysis was performed to confirm the peak points of the functionally abnormal brain areas as the centers of the seeds. Subsequently, the functional connectivity (FC) between these abnormal seeds and other voxels across the whole brain was calculated. Finally, the sex hormone levels, neuroimaging indices and neuropsychological data were combined to detect potential correlations.Results: Compared with the premenopausal controls, the early postmenopausal women exhibited significantly higher serum follicle-stimulating hormone (FSH) levels, more severe climacteric and depressive symptoms, worse sleep quality and more extensive cognitive impairments. Concurrently, the neuroimaging results showed elevated DC values in the left amygdala (AMYG.L), reduced DC values in the left middle occipital gyrus (MOG.L) and right middle occipital gyrus (MOG.R). When we used the AMYG.L as the seed point, FC with the left insula (INS.L), bilateral prefrontal cortex (PFC) and right superior frontal gyrus (SFG.R) was increased; these regions are related to depressive states, poor sleep quality and decreased executive function. When bilateral MOG were used as the seed points, FC with left inferior parietal gyrus (IPG.L), this area closely associated with impaired memory, was decreased.Conclusion: These results illuminated the regional and network-level brain dysfunction in early postmenopausal women, which might provide information on the underlying mechanisms of the different cognitive impairments and emotional alterations observed in this group

Aberrant Brain Function in Active-Stage Ulcerative Colitis Patients: A Resting-State Functional MRI Study

Background: Patients with ulcerative colitis (UC) usually display cognitive impairments, such as memory loss, attention deficits, and declining executive functions, particularly during the active stage of the disease. However, the potential neurological mechanisms of these symptoms remain unclear.Method: Forty-one patients with mildly to moderately active UC, as well as 42 matched healthy controls, were recruited for an examination using psychological scales, cognitive function tests and resting-state functional magnetic resonance imaging (rs-fMRI). Seed points were identified via analysis of amplitude of low-frequency fluctuation (ALFF), and functional connectivity (FC) was calculated between these seed regions and other voxels in the whole brain. Correlation analyses were performed among clinical indexes, neuropsychological assessments and neuroimaging data.Result: Compared with the healthy controls, patients with UC exhibited lower ALFF values in the bilateral hippocampal/parahippocampal (HIPP/ParaHIPP) region and higher ALFF values in the left posterior cingulate cortex (PCC.L) and left middle frontal gyrus (MFG.L). With HIPP/ParaHIPP as the seed point, the strengths of the FC in the bilateral middle frontal gyri (MFG), anterior cingulate cortex (ACC), and left caudate nucleus (CAU.L) increased; using the PCC.L as the seed point, the strengths of the FC in the middle cingulate cortex (MCC) and the left angular gyrus (AUG.L) increased. These abnormal brain regions were mainly located in the limbic system. By analyzing the correlations between these brain regions and behavioral data, we observed a close correlation between decreased HIPP/ParaHIPP activity and memory loss; increased PCC activity and strength of FC with the AUG.L were related to dysfunction of executive function and attention network in patients with UC.Conclusion: Based on these results, the limbic lobe might be the core of the brain-gut axis (BGA) and play an important role in cognitive impairments, suggesting potential mechanisms for cognitive impairment in patients with UC in the active stage of the disease

Developmenrt of EST-SSR and genomic-SSR markers to assess genetic diversity in Jatropha Curcas L.

<p>Abstract</p> <p>Background</p> <p><it>Jatropha curcas L. </it>has attracted a great deal of attention worldwide, regarding its potential as a new biodiesel crop. However, the understanding of this crop remains very limited and little genomic research has been done. We used simple sequence repeat (SSR) markers that could be transferred from <it>Manihot esculenta </it>(cassava) to analyze the genetic relationships among 45 accessions of <it>J. curcas </it>from our germplasm collection.</p> <p>Results</p> <p>In total, 187 out of 419 expressed sequence tag (EST)-SSR and 54 out of 182 genomic (G)-SSR markers from cassava were polymorphic among the <it>J. curcas </it>accessions. The EST-SSR markers comprised 26.20% dinucleotide repeats, 57.75% trinucleotide repeats, 7.49% tetranucleotide repeats, and 8.56% pentanucleotide repeats, whereas the majority of the G-SSR markers were dinucleotide repeats (62.96%). The 187 EST-SSRs resided in genes that are involved mainly in biological and metabolic processes. Thirty-six EST-SSRs and 20 G-SSRs were chosen to analyze the genetic diversity among 45 <it>J. curcas </it>accessions. A total of 183 polymorphic alleles were detected. On the basis of the distribution of these polymorphic alleles, the 45 accessions were classified into six groups, in which the genotype showed a correlation with geographic origin. The estimated mean genetic diversity index was 0.5572, which suggests that our <it>J. curcas </it>germplasm collection has a high level of genetic diversity. This should facilitate subsequent studies on genetic mapping and molecular breeding.</p> <p>Conclusion</p> <p>We identified 241 novel EST-SSR and G-SSR markers in <it>J. curcas</it>, which should be useful for genetic mapping and quantitative trait loci analysis of important agronomic traits. By using these markers, we found that the intergroup gene diversity of <it>J. curcas </it>was greater than the intragroup diversity, and that the domestication of the species probably occurred partly in America and partly in Hainan, China.</p

Cassava genome from a wild ancestor to cultivated varieties

Cassava is a major tropical food crop in the Euphorbiaceae family that has high carbohydrate production potential and adaptability to diverse environments. Here we present the draft genome sequences of a wild ancestor and a domesticated variety of cassava and comparative analyses with a partial inbred line. We identify 1,584 and 1,678 gene models specific to the wild and domesticated varieties, respectively, and discover high heterozygosity and millions of single-nucleotide variations. Our analyses reveal that genes involved in photosynthesis, starch accumulation and abiotic stresses have been positively selected, whereas those involved in cell wall biosynthesis and secondary metabolism, including cyanogenic glucoside formation, have been negatively selected in the cultivated varieties, reflecting the result of natural selection and domestication. Differences in microRNA genes and retrotransposon regulation could partly explain an increased carbon flux towards starch accumulation and reduced cyanogenic glucoside accumulation in domesticated cassava. These results may contribute to genetic improvement of cassava through better understanding of its biology

Multiple Regression Analysis of Influencing Factors on Yield of New Sugarcane Variety Yuetang 03-373

[Objectives] The purpose of this study was to provide reference for cultivation and promotion of a new sugarcane variety Yuetang 03-373, on the basis of analyzing and summarizing the characters of the variety. [Methods] Correlation, multiple regression and path analyses were performed for the yield and yield components of Yuetang 03-373. [Results] Correlation analysis shows that cane yield was significantly correlated with millable stalk number, stalk length and stalk diameter, and among them, the correlation with millable stalk number was the strongest. Multiple regression and path analyses show that millable stalk number contributed the most to cane yield, followed by stalk length, and stalk diameter contributed the least. The regression equation of cane yield against the three yield components was y = -2.871 3 + 1.549 7 x1 + 5.899 0 x2 - 395.429 4 x3 (R = 0.967 2**). [Conclusions] Millable stalk number and stalk length were the important and major factors for high yield of Yuetang 03-373, indicating that Yuetang 03-373 is a sugarcane variety of millable stalk type. In cultivation, full play should be given to the advantage of Yuetang 03-373 in millable stalk number, as well as stalk length (plant height), in order to achieve the purpose of increasing yield

Approaches and Policies to Promote Zero-Waste City Construction: China’s Practices and Lessons

The rapidly growing output of solid waste has brought tremendous pressure to urban development. China launched an action plan known as “Zero-waste city” (ZWC), that refers to an urban development model aimed at reducing the generation of and enhancing the recycling of solid waste, in order to alleviate environmental impacts. Eleven cities and five special zones achieved positive results of solid waste management were selected as pilot areas for exploring empirical methods until 2019. The practices and lessons of the pilot cities need to be deeply analyzed and summarized, so as to promote successful models, learn lessons and better implement the policy comprehensively for other cities. This study presents a review of China’s ZWC policies and practices with constructive suggestions for further development. Based on the policy objective of ZWC and the field investigation of solid waste flow, five crucial approaches to developing ZWC are proposed, namely, solid waste reduction throughout the industrial chain, collaborative treatment of classified municipal solid waste (MSW), efficient utilization of agricultural waste with multiple purposes, safety control of hazardous waste flow, and optimization of market mechanisms. The case study demonstrates that the five paths are appropriate to Xuzhou city. However, deficiencies in MSW classification, pesticide packaging waste collection systems, solid waste product application, management, and policymaking, have emerged. Regulatory prohibitions, extended producer responsibility and market vitality should be adopted to improve the collection, transportation, and utilization of solid waste. Key findings from this research are to summarize crucial paths toward fulfiling ZWC goal, and to reveal some successful practices of, and lessons from ZWC construction by case studies. This study provides a method to further implement zero solid waste management in a targeted manner. The recommendations drawn from the study, which include law, market and institutional measures, may contribute to the achievement of developing sustainable cities

On-Demand Energy Transfer and Energy-Aware Polling-Based MAC for Wireless Powered Sensor Networks

To improve the performance of the wireless powered sensor network (WPSN), this paper proposes a frequency division duplex (FDD)-based on-demand energy transfer protocol and an energy-aware polling-based medium access control (MAC) protocol, called composite energy and data first (CEDF), by using the numbers of data packets and energy packets to determine polling priorities. The performance of the proposed MAC protocol, i.e., CEDF, along with the on-demand energy transfer protocol was evaluated through simulations, with comparison to the closely related protocols such as the round robin (RR) and data first (DF) polling protocols. Compared with RR and DF, our proposed CEDF performs much better in terms of throughput, data packet loss rate, and delay. Additionally, the doubly near&ndash;far problem in WPSNs under our proposed on-demand energy transfer protocol and CEDF was investigated to come up with good solutions to alleviate such a problem

Breeding of Yuetang 08776, a New Sugarcane Variety Suitable for Mechanized Production

[Objectives] To study the breeding of a new sugarcane variety Yuetang 08776 suitable for mechanized production. [Methods] With Yuetang 96-86 as the female parent and Yuetang 99-66 as the male parent, Yuetang 08776 was bred by the Bioengineering Institute of Guangdong Academy of Sciences. It was approved as a non-major crop variety by the Ministry of Agriculture and Rural Affairs in December, 2019. Through many years of multi-pilot trials, the characters, traits and disease resistance of Yuetang 08776 were investigated, and the key points of its cultivation technique were summarized. [Results] Yuetang 08776 is characterized by medium maturity, high sugar content, high and stable yield, strong cold and drought resistance, large number of effective stems, strong ratooning ability and lodging resistance. Yuetang 08776 has excellent agronomic traits, and has been identified as moderately resistant to smut and mosaic disease. The average cane yield of Yuetang 08776 was 119.89 t/ha, 18.50 t/ha higher than that of CK, an increase of 18.25%. The average sugar yield was 17.15 t/ha, 2.11 t/ha higher than that of CK, an increase of 14.03%. The average sucrose content of Yuetang 08776 in the early period (November to December) was 13.20%, 0.71 percentage points lower than that of CK; in the middle and late period (January to March of the following year), the average sucrose content was 15.23%, 0.22 percentage points lower than that of CK; and the average sucrose content of the whole period was 14.21%, 0.47 percentage points lower than that of CK. [Conclusions] Yuetang 08776 is a sugarcane variety with medium maturity, high sugar, high and stable yield, strong resistance and good ratooning. It is suitable for mechanized seeding, management and harvesting, and suitable for planting and popularizing in most sugarcane production areas of Guangdong, Guangxi and Yunnan

High lymphatic vessel density and presence of lymphovascular invasion both predict poor prognosis in breast cancer

Abstract Background Lymphatic vessel density and lymphovascular invasion are commonly assessed to identify the clinicopathological outcomes in breast cancer. However, the prognostic values of them on patients’ survival are still uncertain. Methods Databases of PubMed, Embase, and Web of Science were searched from inception up to 30 June 2016. The hazard ratio with its 95% confidence interval was used to determine the prognostic effects of lymphatic vessel density and lymphovascular invasion on disease-free survival and overall survival in breast cancer. Results Nineteen studies, involving 4215 participants, were included in this study. With the combination of the results of lymphatic vessel density, the pooled hazard ratios and 95% confidence intervals were 2.02 (1.69–2.40) for disease-free survival and 2.88 (2.07–4.01) for overall survival, respectively. For lymphovascular invasion study, the pooled hazard ratios and 95% confidence intervals were 1.81 (1.57–2.08) for disease-free survival and 1.64 (1.43–1.87) for overall survival, respectively. In addition, 29.56% (827/2798) of participants presented with lymphovascular invasion in total. Conclusions Our study demonstrates that lymphatic vessel density and lymphovascular invasion can predict poor prognosis in breast cancer. Standardized assessments of lymphatic vessel density and lymphovascular invasion are needed