Additional file 1: of A potential gliovascular mechanism for microglial activation: differential phenotypic switching of microglia by endothelium versus astrocytes

Figure S1. Cell viability of endothelial cells and astrocytes after OGD for 4 h and reoxygenation for 24 h. (PDF 56 kb

Additional file 4: of A potential gliovascular mechanism for microglial activation: differential phenotypic switching of microglia by endothelium versus astrocytes

Figure S4. (a) IgG immunostaining of the brain sections of rat transient MCAo models at 1, 3, and 7 days after ischemia. Data were expressed as mean ± SEM. *p < 0.05 (one-way ANOVA). (b) GFAP immunostaining of the brain sections of rat transient MCAo models at 1, 3, and 7 days after ischemia. (PDF 3094 kb

Additional file 3: of A potential gliovascular mechanism for microglial activation: differential phenotypic switching of microglia by endothelium versus astrocytes

Figure S3. Gene expression of microglia sorted from the ischemic brains by FACS was detected using real-time PCR at different time points after ischemia. (PDF 75 kb

Expression of disease-related genes in the vasculome of mouse brain.

<p>Expression of disease-related genes in the vasculome of mouse brain.</p

Protein-protein interaction (PPI) networks in the vasculome of mouse brain.

<p><b>A,</b> PPI network for leukocyte transendothelial migration. <b>B,</b> PPI network for the WNT signaling pathway. <b>C,</b> PPI network for adherence junctions. The expression levels of genes in the vasculome of mouse brain are indexed by color.</p

Enriched pathways detected in the vasculome of mouse brain.

<p>Note: Analysis based on brain endothelial specific genes in the mouse brain vasculome. These enriched pathways suggest that specific pathways and mechanisms are selectively enhanced in brain compared to heart and kidney glomerular vasculomes.</p

Expression of plasma proteins in the vasculome of mouse brain.

<p>Note: *core is the intersect of all 4 independent data set. Lists of circulating proteins in human plasma were compiled from 4 different proteomic studies, then each study was overlapped with the expression profile of the brain vasculome. A core set of 387 proteins were defined as common proteins detected in all 4 human plasma protein studies. Out of the core set of plasma proteins, 100 proteins were expressed in the brain vasculome.</p

The vasculome of mouse brain is unique and different from those found in mouse heart and kidney.

<p>Heatmap for visualization of the expression levels of organ-specific endothelial genes across brain, heart and kidney glomeruli. X-axis represents individual samples and y-axis represents different genes. The expression levels of genes are indexed by color.</p

Angiogenesis networks.

<p><b>A</b>, Protein-protein interaction network for angiogenesis in the vasculome of mouse brain (including nearest neighbors). Circles for genes in angiogenesis and squares for the neighbor genes. The expression levels of genes in the vasculome of mouse brain are indexed by color. <b>B</b>, Heatmap comparison of expression profiles of genes in the VEGF signaling pathway from the vasculome of mouse brain, heart and kidney glomeruli. The expression levels of genes are indexed by color.</p

sj-pdf-1-jcb-10.1177_0271678X231197392 - Supplemental material for Increased task-relevant fMRI responsiveness in comatose cardiac arrest patients is associated with improved neurologic outcomes

Supplemental material, sj-pdf-1-jcb-10.1177_0271678X231197392 for Increased task-relevant fMRI responsiveness in comatose cardiac arrest patients is associated with improved neurologic outcomes by Kiran Dhakal, Eric S Rosenthal, Annelise M Kulpanowski, Jacob A Dodelson, Zihao Wang, Gaston Cudemus-Deseda, Marjorie Villien, Brian L Edlow, Alexander M Presciutti, James L Januzzi, MingMing Ning, W Taylor Kimberly, Edilberto Amorim, M Brandon Westover, William A Copen, Pamela W Schaefer, Joseph T Giacino, David M Greer and Ona Wu in Journal of Cerebral Blood Flow & Metabolism</p