Two new triterpenoid saponins from <i>Ilex cornuta</i>

<div><p>Two new triterpenoid saponins (<b>1</b> and <b>2</b>) were isolated from the stems of <i>Ilex cornuta</i>, along with two known triterpenoids (<b>3</b> and <b>4</b>). The structures of compounds <b>1</b> and <b>2</b> were determined as ursane-12,19-diene-28-oic acid 3β-<i>O</i>-β-d-glucuronopyranoside-6-<i>O</i>-methyl ester (<b>1</b>), 3α,23α-dihydroxy-olean-9(11),12-diene-28-oic acid 28-<i>O</i>-β-d-glucopyranosyl ester (<b>2</b>), on the basis of hydrolysis and spectral evidence, including 1D and 2D NMR and high resolution electrospray ionization mass spectrometry (HR-ESI-MS) analyses. Protective effects of compounds <b>1</b>–<b>4</b> were tested against H₂O₂-induced H9c2 cardiomyocyte injury, and the data showed that all these compounds had no cell-protective effect.</p></div