TRIM28-Regulated Transposon Repression Is Required for Human Germline Competency and Not Primed or Naive Human Pluripotency.

Transition from primed to naive pluripotency is associated with dynamic changes in transposable element (TE) expression and demethylation of imprinting control regions (ICRs). In mouse, ICR methylation and TE expression are each regulated by TRIM28; however, the role of TRIM28 in humans is less clear. Here, we show that a null mutation in TRIM28 causes significant alterations in TE expression in both the naive and primed states of human pluripotency, and phenotypically this has limited effects on self-renewal, instead causing a loss of germline competency. Furthermore, we discovered that TRIM28 regulates paternal ICR methylation and chromatin accessibility in the primed state, with no effects on maternal ICRs. Taken together, our study shows that abnormal TE expression is tolerated by self-renewing human pluripotent cells, whereas germline competency is not

Effectiveness of RSS feed item duplication detection using word matching

Users of feed aggregators know that duplicated articles are found occasionally on the feeds they subscribe to. It can be time consuming to read all articles and stumble upon duplicated items they have already read. Our work here is to determine the effectiveness of using basic word matching to remove duplicated items and only show the most relevant item, thus saving readers’ time. The method described in this paper to remove duplicates involves word matching heuristics with an appropriate matching percentage. The duplicated feeds are then ranked to only display the highest ranked article. Ranking is done using the number of search items found on the titles of the news feeds where the highest number returned will be considered the highest ranked article. Using Malaysian online news feeds, our method found that with a matching percentage of 40%, our method will be able to minimize duplicates effectively with minimal errors. We did further empirical studies using 9 technology blog feeds over a longer period to provide us with a better averaging results. The matching percentage obtained is also within the same quantum. The method described here has a low overhead in terms of processing for the duplicates and with careful selection of matching percentage, the system will effectively remove the majority of duplicate

ROC Analysis for Phase II Group Sequential Basket Clinical Trial

The basket trial is a recent development in the clinical trial practice. It conducts the test of the same treatment on several different related diseases in a single trial, and has the advantage of reduced cost and enhanced efficiency. A natural question is how to assess the performance of the group sequential basket trial against the classical group sequential trial? To our knowledge, a formal assessment hasn't been seen in the literature, and is the goal of this study. Specifically, we use the receiver operating characteristic curve to assess the performance of the mentioned two trials. We considered two cases, parametric and nonparametric settings. The former is efficient when the parametric model is correctly specified, but can bemis-leading if the model is incorrect; the latter is less efficient but is robust in that it cannot be wrong no matter what the true data generating model is. Simulation studies are conducted to evaluate the experiments, and it suggests that the group sequential basket trial generally outperforms the group sequential trial in either the parametric and nonparametric cases, and that the nonparametric method gives more accurate evaluation than the parametric one for moderate to large sample sizes

Crystal structure of 1-{(Z)-[(2E)-3-(4-chlorophenyl)-1-phenylprop-2-en-1-ylidene]amino}-3-ethylthiourea

In the title thiosemicarbazone compound, C18H18ClN3S, the CN3S residue is almost planar (r.m.s. deviation = 0.0031 Å) and forms dihedral angles of 65.99 (7) and 34.60 (10)° with the phenyl and chlorobenzene rings, respectively; the dihedral angle between the aromatic rings is 85.13 (8)°. The conformation about the C=N bond is Z, and that about the C=C bonds is E. The imine N and ethyl N atoms are syn and are linked by an ethyl–imine N—H...N hydrogen bond. This H atom also forms an intermolecular hydrogen bond to the thione S atom, resulting in a supramolecular helical chain propagating along the b axis. The chains are consolidated into a three-dimensional architecture by phenyl-C—H...Cl contacts and weak π–π interactions between centrosymmetrically related chlorobenzene rings [inter-centroid distance = 3.9127 (15) Å]

Uncertainty-aware Gait Recognition via Learning from Dirichlet Distribution-based Evidence

Existing gait recognition frameworks retrieve an identity in the gallery based on the distance between a probe sample and the identities in the gallery. However, existing methods often neglect that the gallery may not contain identities corresponding to the probes, leading to recognition errors rather than raising an alarm. In this paper, we introduce a novel uncertainty-aware gait recognition method that models the uncertainty of identification based on learned evidence. Specifically, we treat our recognition model as an evidence collector to gather evidence from input samples and parameterize a Dirichlet distribution over the evidence. The Dirichlet distribution essentially represents the density of the probability assigned to the input samples. We utilize the distribution to evaluate the resultant uncertainty of each probe sample and then determine whether a probe has a counterpart in the gallery or not. To the best of our knowledge, our method is the first attempt to tackle gait recognition with uncertainty modelling. Moreover, our uncertain modeling significantly improves the robustness against out-of-distribution (OOD) queries. Extensive experiments demonstrate that our method achieves state-of-the-art performance on datasets with OOD queries, and can also generalize well to other identity-retrieval tasks. Importantly, our method outperforms the state-of-the-art by a large margin of 51.26% when the OOD query rate is around 50% on OUMVLP

Bis(N′-{(E)-[(2E)-1,3-diphenylprop-2-en-1-ylidene]amino}-N-ethylcarbamimidothioato-κ2 N′,S)zinc(II): crystal structure and Hirshfeld surface analysis

The title ZnII complex, [Zn(C18H18N3S)2], (I), features two independent but chemically equivalent molecules in the asymmetric unit. In each, the thiosemicarbazonate monoanion coordinates the ZnII atom via the thiolate-S and imine-N atoms, with the resulting N2S2 donor set defining a distorted tetrahedral geometry. The five-membered ZnSCN2 chelate rings adopt distinct conformations in each independent molecule, i.e. one ring is almost planar while the other is twisted about the Zn—S bond. In the crystal, the two molecules comprising the asymmetric unit are linked by amine-N—H...N(imine) and amine-N—H...S(thiolate) hydrogen bonds via an eight-membered heterosynthon, {...HNCN...HNCS}. The dimeric aggregates are further consolidated by benzene-C—H...S(thiolate) interactions and are linked into a zigzag supramolecular chain along the c axis via amine-N—H...S(thiolate) hydrogen bonds. The chains are connected into a three-dimensional architecture via phenyl-C—H...π(phenyl) and π–π interactions, the latter occurring between chelate and phenyl rings [inter-centroid separation = 3.6873 (11) Å]. The analysis of the Hirshfeld surfaces calculated for (I) emphasizes the different interactions formed by the independent molecules in the crystal and the impact of the π–π interactions between chelate and phenyl rings

Bis(4-methoxychalcone 4-ethylthiosemicarbazonato-κ2 N 1,S)zinc(II): Crystal structure and Hirshfeld surface analysis

The title ZnII complex, [Zn(C19H20N3OS)2] {systematic name: bis[(N-ethyl-N0-{(Z)-[(2E)-3-(4-methoxyphenyl)-1-phenylprop-2-en-1-ylidene]amino}carbamimidoyl)sulfanido]zinc(II)}, features a tetrahedrally coordinated ZnII ion within an N2S2 donor set provided by two N,S-chelating thiosemicarbazone anions. The resulting five-membered Zn,C,N2,S chelate rings adopt different conformations, i.e. almost planar and an envelope with the Zn atom being the flap atom. The configuration about the imine bond within the chelate ring is Z but those about the exocyclic imine and ethylene bonds are E. In the crystal, supramolecular [100] chains mediated by thioamide-N—H...S(thione) hydrogen bonds and eight-membered thioamide {.....HNCS}2 synthons are observed. A range of interactions, including C—H...O, C—H...., C—H....(chelate ring) and .(methoxybenzene)—.(chelate ring) consolidate the packing. The Hirshfeld surface analysis performed on the title complex also indicates the influence of the interactions involving the chelate rings upon the packing along with the more conventional contacts