Electrochemical detection techniques in micro- and nanofluidic devices

© 2014, Springer-Verlag Berlin Heidelberg. Electrochemical techniques are widely used in microfluidic and nanofluidic devices because they are suitable for miniaturization, have better sensitivity compared to optical detection techniques, and their components can be reliably microfabricated. In addition to the detection and quantification of analytes, electrochemical techniques can be used to monitor processes such as biological cell death and protein/DNA separations/purifications. Such techniques are combined with micro- and nanofluidic devices with point-of-care (POC) applications in mind, where cost, footprint, ease of use, and independence from peripheral equipment are critical for a viable design. A large variety of electrode materials and device configurations have been employed to meet these requirements. This review introduces the reader to the major electrochemical techniques, materials, and fabrication methods for working and reference electrodes, and to surface modifications of electrodes to facilitate electrochemical measurements, in the context of micro- and nanofluidic devices. The continuing development of these techniques holds promise for the next-generation lab-on-a-chip devices, which can realize the goals of this technology such as POC clinical analysis

BioMEMS and Electrophoresis in 2006: Review of the 23rd Annual Meeting of the American Electrophoresis Society

The 23rd Annual Meeting of the American Electrophoresis Society (AES) was held at the San Francisco Hilton in San Francisco, California on 12–17 November 2006. This year’s meeting featured a look toward the future, with an emphasis on theoretical and experimental advances in miniaturization of BioMEMS, electrokinetics, and proteomics technologies. A total of 13 sessions accommodating 71 presentations and 18 posters were held in conjunction with the Annual Meeting of the American Institute of Chemical Engineers (AIChE). This review and corresponding special issue of Biomicrofluidics provide a sampling of some of the exciting research presented at the conference

Recruitment and Retention of International Graduate Students in US Universities

International students account for 3.5% of graduate students in the US with 17% enrolled in engineering programs in 2008. Recruiting and retention of International Graduate Students (IGS) is an essential and important consideration for US universities with increased competition from other countries. Understanding concerns and preferences influencing the IGS’s decision to choose a school in addition to factors affecting decisions to continue advanced degrees at a university will help schools effectively recruit and retain better quality students and thus increase research productivity. This paper addresses the design of comprehensive online survey to find preferences and influential factors affecting the decision of IGS in choosing a graduate school. IGS and alumni at US schools were invited anonymously to participate and complete the survey. Survey results were analyzed and interpreted both quantitatively and qualitatively. Results indicate that preferences of IGS to choose a school depend on their nationality, gender, age and several other factors. The paper concludes with recommendations to improve recruitment and retention, considering preferences and concerns of different groups of IGS and strategizing the efforts in view of diversity of IGS

Spatially variant red blood cell crenation in alternating current non-uniform fields

© 2014 AIP Publishing LLC. Alternating-current (AC) electrokinetics involve the movement and behaviors of particles or cells. Many applications, including dielectrophoretic manipulations, are dependent upon charge interactions between the cell or particle and the surrounding medium. Medium concentrations are traditionally treated as spatially uniform in both theoretical models and experiments. Human red blood cells (RBCs) are observed to crenate, or shrink due to changing osmotic pressure, over 10min experiments in non-uniform AC electric fields. Cell crenation magnitude is examined as functions of frequency from 250 kHz to 1 MHz and potential from 10 Vpp to 17.5 Vpp over a 100 lm perpendicular electrode gap. Experimental results show higher peak to peak potential and lower frequency lead to greater cell volume crenation up to a maximum volume loss of 20%. A series of experiments are conducted to elucidate the physical mechanisms behind the red blood cell crenation. Non-uniform and uniform electrode systems as well as high and low ion concentration experiments are compared and illustrate that AC electroporation, system temperature, rapid temperature changes, medium pH, electrode reactions, and convection do not account for the crenation behaviors observed. AC electroosmotic was found to be negligible at these conditions and AC electrothermal fluid flows were found to reduce RBC crenation behaviors. These cell deformations were attributed to medium hypertonicity induced by ion concentration gradients in the spatially nonuniform AC electric fields

DC insulator dielectrophoretic applications in microdevice technology: A review

Dielectrophoresis is a noninvasive, nondestructive, inexpensive, and fast technique for the manipulation of bioparticles. Recent advances in the field of dielectrophoresis (DEP) have resulted in new approaches for characterizing the behavior of particles and cells using direct current (DC) electric fields. In such approaches, spatial nonuniformities are created in the channel by embedding insulating obstacles in the channel or flow field in order to perform separation or trapping. This emerging field of dielectrophoresis is commonly termed DC insulator dielectrophoresis (DC-iDEP), insulator-based dielectrophoresis (iDEP), or electrodeless dielectrophoresis (eDEP). In many microdevices, this form of dielectrophoresis has advantages over traditional AC-DEP, including single material microfabrication, remotely positioned electrodes, and reduced fouling of the test region. DC-iDEP applications have included disease detection, separation of cancerous cells from normal cells, and separation of live from dead bacteria. However, there is a need for a critical report to integrate these important research findings. The aim of this review is to provide an overview of the current state-of-art technology in the field of DC-iDEP for the separation and trapping of inert particles and cells. In this article, a review of the concepts and theory leading to the manipulation of particles via DC-iDEP is given, and insulating obstacle geometry designs and the characterization of device performance are discussed. This review compiles and compares the significant findings obtained by researchers in handling and manipulating particles. © 2010 Springer-Verlag

Solution pH change in non-uniform alternating current electric fields at frequencies above the electrode charging frequency

© 2014 AIP Publishing LLC. AC Faradaic reactions have been reported as a mechanism inducing non-ideal phenomena such as flow reversal and cell deformation in electrokinetic microfluidic systems. Prior published work described experiments in parallel electrode arrays below the electrode charging frequency (fc), the frequency for electrical double layer charging at the electrode. However, 2D spatially non-uniform AC electric fields are required for applications such as in plane AC electroosmosis, AC electrothermal pumps, and dielectrophoresis. Many microscale experimental applications utilize AC frequencies around or above fc. In this work, a pH sensitive fluorescein sodium salt dye was used to detect [H+] as an indicator of Faradaic reactions in aqueous solutions within non-uniform AC electric fields. Comparison experiments with (a) parallel (2D uniform fields) electrodes and (b) organic media were employed to deduce the electrode charging mechanism at 5?kHz (1.5fc). Time dependency analysis illustrated that Faradaic reactions exist above the theoretically predicted electrode charging frequency. Spatial analysis showed [H+] varied spatially due to electric field non-uniformities and local pH changed at length scales greater than 50??m away from the electrode surface. Thus, non-uniform AC fields yielded spatially varied pH gradients as a direct consequence of ion path length differences while uniform fields did not yield pH gradients; the latter is consistent with prior published data. Frequency dependence was examined from 5 kHz to 12?kHz at 5.5 Vpp potential, and voltage dependency was explored from 3.5 to 7.5 Vpp at 5?kHz. Results suggest that Faradaic reactions can still proceed within electrochemical systems in the absence of well-established electrical double layers. This work also illustrates that in microfluidic systems, spatial medium variations must be considered as a function of experiment time, initial medium conditions, electric signal potential, frequency, and spatial position

Changes in Membrane Dielectric Properties of Porcine Kidney Cells Provide Insight into the Antiviral Activity of Glycine

The ability to monitor the status and progression of viral infections is important for development and screening of new antiviral drugs. Previous research illustrated that the osmolyte glycine (Gly) reduced porcine parvovirus (PPV) infection in porcine kidney (PK-13) cells by stabilizing the capsid protein and preventing virus capsid assembly into viable virus particles. Dielectrophoresis (DEP) was examined herein as a noninvasive, electric field- and frequency-dependent tool for real-time monitoring of PK-13 cell responses to obtain information about membrane barrier functionality and polarization. DEP responses of PK-13 cells were compared to those of PPV-infected cells in the absence and presence of the osmolyte glycine. With infection progression, PK-13 DEP spectra shifted toward lower frequencies, reducing crossover frequencies (fCO). The spherical single-shell model was used to extract PK-13 cell dielectric properties. Upon PPV infection, specific membrane capacitance increased over the time progression of virus attachment, penetration, and capsid protein production and assembly. Following glycine treatment, the DEP spectra displayed attenuated fCO and specific membrane capacitance values shifted back toward uninfected PK-13 cell values. These results suggest that DEP can be used to noninvasively monitor the viral infection cycle and screen antiviral compounds. DEP can augment traditional tools by elucidating membrane polarization changes related to drug mechanisms that interrupt the virus infection cycle

Analyzing self-reported challenges and preferences for enhancing recruitment and retention of international Female Graduate Engineering Students

Recruitment and retention of graduate students are well-recognized needs for higher education institutions. Particularly, increasing women students is one of the major priorities, as they are under-represented, especially in engineering fields. There is a need to identify the factors influencing female students in choosing a school and handling challenges during their graduate study. This paper addresses the preferences to choose a graduate school and challenges faced by current international women graduate engineering students at US universities. An online survey was conducted and analyzed to identify female students\u27 concerns and influential factors before applying to or attending a graduate school. Results indicate that female students tend to choose a school on a different set of criteria compared to their male colleagues. Statistical analyses showed female students having concerns with language and culture change more than males. Furthermore, female students also reported location of university and funding opportunities as more important in choosing a school. Female graduate students reported in-school issues, such as courses, funding, and adjusting to culture, as the most challenging phase they experienced, significantly more than male students reported. There is a difference in perceptions among female and male students for choosing a school and handling challenges during graduate study. One of the key factors in increasing female students\u27 recruitment and retention was found to be communication and mentorship. Another key factor was providing some sort of financial stability before they entered a graduate program. The main reasons female graduate students transferred to a different university were faculty issues and funding. We provide examples of how these findings can be incorporated by universities in recruitment and retention strategies to attract more international women graduate students in engineering programs. © 2013 by Begell House, Inc

Development of a 3D graphene electrode dielectrophoretic device

The design and fabrication of a novel 3D electrode microdevice using 50 μm thick graphene paper and 100 μm double sided tape is described. The protocol details the procedures to construct a versatile, reusable, multiple layer, laminated dielectrophoresis chamber. Specifically, six layers of 50 μm × 0.7 cm × 2 cm graphene paper and five layers of double sided tape were alternately stacked together, then clamped to a glass slide. Then a 700 μm diameter micro-well was drilled through the laminated structure using a computer-controlled micro drilling machine. Insulating properties of the tape layer between adjacent graphene layers were assured by resistance tests. Silver conductive epoxy connected alternate layers of graphene paper and formed stable connections between the graphene paper and external copper wire electrodes. The finished device was then clamped and sealed to a glass slide. The electric field gradient was modeled within the multi-layer device. Dielectrophoretic behaviors of 6 μm polystyrene beads were demonstrated in the 1 mm deep micro-well, with medium conductivities ranging from 0.0001 S/m to 1.3 S/m, and applied signal frequencies from 100 Hz to 10 MHz. Negative dielectrophoretic responses were observed in three dimensions over most of the conductivity-frequency space and cross-over frequency values are consistent with previously reported literature values. The device did not prevent AC electroosmosis and electrothermal flows, which occurred in the low and high frequency regions, respectively. The graphene paper utilized in this device is versatile and could subsequently function as a biosensor after dielectrophoretic characterizations are complete