Toxicity assessment of long-term exposure to non-thermal plasma activated water in mice

Non-thermal plasma activated water (PAW) has recently emerged as a powerful antimicrobial agent. Despite numerous potential bio-medical applications, studies concerning toxicity in live animals, especially after long-term exposure, are scarce. Our study aimed to assess the effects of long-term watering with PAWon the health of CD1 mice. PAWwas prepared from distilled water with a GlidArc reactor according to a previously published protocol. The pH was 2.78. The mice received PAW(experimental group) or tap water (control group) daily for 90 days as the sole water source. After 90 days, the following investigations were performed on the euthanatized animals: gross necropsy, teeth mineral composition, histopathology, immunohistochemistry, hematology, blood biochemistry, methemoglobin level and cytokine profile. Mice tolerated PAWvery well and no adverse effects were observed during the entire period of the experiment. Histopathological examination of the organs and tissues did not reveal any structural changes. Moreover, the expression of proliferation markers PCNA and Ki67 has not been identified in the epithelium of the upper digestive tract, indicating the absence of any pre- or neoplastic transformations. The results of our study demonstrated that long-term exposure to PAWcaused no toxic effects and could be used as oral antiseptic solution in dental medicine

CRiSIS 2011 : proceedings of the sixth International Conference on Risks and Security of Internet and Systems, Timisoara, september 26-28

International audienceSeveral trends in computer security have become prominent since the beginning of the new century and are represented in the program. They include the proliferation of intrusions that exploit new vulnerabilities, the necessity to respond to an increasing number of computer security incidents, the emergence of new security threats and the need to adapt existing approaches and models to handle these threats. Reflecting these trends, the conference includes sessions on attacks and defenses, security of resource-constrained devices, trust and quality of security management, and advanced security models

PHARMACOLOGICAL VERSUS PSYCHOLOGICAL TREATMENT IN ODONTOPHOBIA: THE RIGHT APPROACH

This review synthesizes existing literature on the comparative effectiveness of psychotherapy versus pharmacotherapy in treating odontophobia. It critically examines various studies and clinical trials that explore the outcomes of cognitive-behavioral therapy (CBT), exposure therapy, and other psychotherapeutic interventions, against pharmacological approaches like anxiolytics and sedatives in managing dental anxiety. The review assesses the efficacy, sustainability, and patient acceptability of these treatments, focusing on anxiety reduction, improvement in dental visit compliance, and long-term management of odontophobia. By integrating findings from diverse studies, this review aims to elucidate the most effective treatment modality, offering valuable guidance for clinicians in the optimized management of odontophobia

Etiologic Agents and Antifungal Susceptibility of Oral Candidosis from Romanian patients with HIV-infection or type 1 diabetes mellitus

This is the first Romanian investigation of oral candidosis in patients suffering of HIV-infection or type 1 diabetes mellitus (T1DM).Candida albicans was the dominant species in both types of isolates: n = 14 (46.7%) in T1DM, n = 60 (69.8%) in HIV. The most frequent non-albicans Candida spp. were Candida kefyr (n = 6; 20%) in T1DM and Candida dubliniensis (n = 8; 9.3%) in HIV. Resistance to fluconazole was detected only in the HIV non-albicans Candida group (n = 8; 9.3%). All isolates were susceptible to VOR. The experimental drug MXP had MIC values equal or close to the ones of VOR. Echinocandin resistance was more frequent than azole resistance

THE ROLE OF MITOCHONDRIAL CALCIUM OVERLOAD IN CYCLOSPORINE A-INDUCED GINGIVAL HYPERPLASIA

Aim of the study Apoptosis (programmed cell death) is a possible mechanism of cyclosporine A (CsA)-induced gingival hyperplasia. The purpose of this paper is to study the effect of ionomycin and ionophore A23187 on normal gingival fibroblasts and also on fibroblasts treated with CsA using flow cytometry methods. Material and methods All of our experiments were performed on fibroblasts obtained from gingival male rats through explant technique and the opening of the mitochondrial transient permeability pore as a result of calcium cytosol overload was followed using calcein AM and CoCl2. Results Our results show a significant difference between normal gingival fibroblasts and those treated with CsA when using ionophore A23187. On the other hand, ionomycin hasn’t significant effects on mitochondrial calcein load in normal or treated fibroblasts. Conclusions: Cytosolic calcium overload is one of the mechanisms involved in drug-induced gingival hyperplasia

Genotype comparison of Candida albicans isolates from different clinical samples

Background: Fungal infections are a health issue paradoxically fuelled by the developments in medical care. Objectives: Our study is an investigation on the correlation between the infection site and the genotypes of Candida albicans strains isolated from Romanian patients. Methods: A total number of 301 isolates from different clinical sites were investigated in terms of genotype determination. Results: The isolates were clustered in three groups according to their genotype: 55.81% showed genotype A, 14.62% genotype B, and 29.57% genotype C. Conclusions: No significant correlation was found between the genotype and the infection site, but a significant correlation was found between genotype C and isolates from HIV patients proving that C. albicans pathogenicity probably relies on factors related to the host

Phenotypic and genotypic variations in Candida albicans isolates from Romanian patients

Background: During the last two decades a major increase in the proportion of severe fungal infections has been noted due to the excessive use of broad-spectrum antibiotics, catheters, and a growing number of immunocompromised patients