Liposome loaded doxorubicin delivery to cells via sonoporation

Biochemijos katedraBiologijos katedraVytauto Didžiojo universiteta

Investigation of microbubble cavitation-induced calcein release from cells in vitro

In the present study, microbubble (MB) cavitation signal analysis was performed together with calcein release evaluation in both pressure and exposure duration domains of the acoustic field. A passive cavitation detection system was used to simultaneously measure MB scattering and attenuation signals for subsequent extraction efficiency relative to MB cavitation activity. The results indicate that the decrease in the efficiency of extraction of calcein molecules from Chinese hamster ovary cells, as well as cell viability, is associated with MB cavitation activity and can be accurately predicted using inertial cavitation doses up to 0.18 V × s (R2 > 0.9, p < 0.0001). No decrease in additional calcein release or cell viability was observed after complete MB sonodestruction was achieved. This indicates that the optimal exposure duration within which maximal sono-extraction efficiency is obtained coincides with the time necessary to achieve complete MB destruction. These results illustrate the importance of MB inertial cavitation in the sono-extraction process. To our knowledge, this study is the first to (i) investigate small molecule extraction from cells via sonoporation and (ii) relate the extraction process to the quantitative characteristics of MB cavitation acoustic spectraBiologijos katedraGamtos mokslų fakultetasKauno technologijos universitetasVytauto Didžiojo universiteta

The investigation of calcein sonoextraction from cells in vitro

This work was funded by Research Council of Lithuania project SVE-08/2014In current study sonoporation induced calcein release efficiency was evaluated altogether with cell viability. The results show that the efficiency of the extraction of small molecules from cells occurs at relatively high cell viability. These results imply transient pores to be the dominant pathway for small molecules to cross cell membrane during sonoporationBiochemijos katedraBiologijos katedraGamtos mokslų fakultetasKauno technologijos universitetasVytauto Didžiojo universiteta

The influence of ultrasound parameters on cell viability and sonoporation effectiveness

Biologijos katedraKauno technologijos universitetasVytauto Didžiojo universiteta