15 research outputs found
Oligonucleotide synthesis onto poly(N-acryloylmorpholine-co-N-acryloxysuccinimide): Assessment of the resulting conjugates in a DNA sandwich hybridization test
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A polyphenylene dendrimer-detergent complex as a highly fluorescent probe for bioassays
No full textThe synthesis of a polyphenylene dendrimer carrying three perylenemonoimide dyes as well as one biotin group is presented. Due to the hydrophobic polyphenylene scaffold, this dendrimer is insoluble in water thus preventing investigations in aqueous media. However, the use of an appropriate detergent results in the formation of well-defined supramolecular dendrimer-detergent complexes being soluble in aqueous media. The dendrimer-detergent complexes have a constant hydrodynamic radius of 7.1 nm measured by light scattering and fluorescence correlation spectroscopy and exhibit a high stability in the presence of blood serum proteins. The specific binding of the dendrimer-detergent complexes carrying a single biotin group to the protein streptavidin is demonstrated using a magnetic bead assay
A polyphenylene dendrimer-detergent complex as a highly fluorescent probe for bioassays
No full textThe synthesis of a polyphenylene dendrimer carrying three perylenemonoimide dyes as well as one biotin group is presented. Due to the hydrophobic polyphenylene scaffold, this dendrimer is insoluble in water thus preventing investigations in aqueous media. However, the use of an appropriate detergent results in the formation of well-defined supramolecular dendrimer-detergent complexes being soluble in aqueous media. The dendrimer-detergent complexes have a constant hydrodynamic radius of 7.1 nm measured by light scattering and fluorescence correlation spectroscopy and exhibit a high stability in the presence of blood serum proteins. The specific binding of the dendrimer-detergent complexes carrying a single biotin group to the protein streptavidin is demonstrated using a magnetic bead assay
Oligonucleotide−Polymer Conjugates: Effect of the Method of Synthesis on Their Structure and Performance in Diagnostic Assays
No full textAutomated Oligonucleotide Solid-Phase Synthesis on Nanosized Silica Particles Using Nano-on-Micro Assembled Particle Supports
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Site-Specific Labeling of Surface Proteins on Living Cells Using Genetically Encoded Peptides that Bind Fluorescent Nanoparticle Probes
No full textSilica Nanoparticle Architecture Determines Radiative Properties of Encapsulated Fluorophores
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