infarction and non-ST-elevated myocardial infarction via LC/Q-TOF/MS/MS

Acute coronary syndrome (ACS) is a clinical condition caused by a disturbance in myocardial blood flow. ACS can be basically divided into two forms: ST elevation myocardial infarction (STEMI) due to complete occlusion of the coronary artery and non-ST elevation myocardial infarction (NSTEMI) due to partial occlusion of the coronary artery. In this study, we aimed to monitor the metabolite profile of STEMI and NSTEMI patients and compare the results via untargeted metabolomics approach. Serum samples were collected from STEMI and NSTEMI patients, and each group consists of 20 participants. Extraction was achieved by acetonitrile, and chromatographic separation was performed by LC/Q-TOF/MS/MS accompanied with dual AJS ESI positive ion mode. METLIN, MATLAB 2017a-PLS Toolbox7.2, and Human Metabolome Database were utilized for bioinformatics evaluation of obtained findings. In our results, 203 m/z ratio was detected and 163 m/z ratio passed the significance criteria (fold analysis > 1.5 and p < 0.05). Twenty-five metabolites including BCAAs, LysoPC species, lactic acid, succinate, malonic acid, maleic acid, butyric acid, carnitine, and betaine were identified. In conclusion, new biomarker candidates were identified to differentiate the diagnosis of STEMI and NSTEMI. Identified metabolites are indicative of alterations in oxidative stress, hypoxia, TCA cycle, and amino acid metabolism.C1 [Gundogdu, Gulsah] Pamukkale Univ, Fac Med, Dept Physiol, TR-20070 Denizli, Turkey.[Miloglu, Fatma Demirkaya; Senol, Onur] Ataturk Univ, Analyt Chem Dept, Eczacilik Fak, Erzurum, Turkey.[Koza, Yavuzer; Gundogdu, Fuat] Ataturk Univ, Cardiol Dept, Tip Fak, Erzurum, Turkey

Quantification and Anti-Cancer Activity on SH-SY5Y Neuroblastoma Cells

Background: Eremurus spectabilis BIEB. (Liliaceae) is an edible and medicinal plant in Turkey.Introduction: This study was designed to isolate and quantify isoorientin in leaves of E. spectabilis and exhibit its effect on SH-SY5Y neuroblastoma cell line.Methods: Purity and identification of isoorientin were evaluated by 1D-NMR, 2D-NMR and Q-TOF were isolated from E. spectabilis via column chromatography. An HPLC method was also developed and validated for isoorientin.Results: Quantitative measurements indicated that contents of isoorientin in E. spectabilis leaves were 81.01 mg/g and MeOH extract were 23.75 mg/gr. All measurements were performed at 350 nm. Anti-cancer activity was investigated on cell culture. IC50 doses of isoorientin were detected as 250 mu M at the 48th hour in SH-SY5Y cells by XTT assay. Real-time PCR analysis in SH-SY5Y cells showed that CCND1, CDK6, casp-9, Bax, ATR, Bcl-2, CHEK1 and CHEK2, expressions significantly reduced in experimental group when compared with the control group. p53, p21, caspase-3, caspase-8, Bcl-2, ATM and ERCCI expressions increased in the experimental group when compared with the control group (P<0.05).Conclusion: Measurements revealed that E. spectabilis contains high amount of isoorientin and can be used as a new source of this compound. In addition to this, isoorientin affects cell proliferation of neuroblastoma cells by cell cycle control and apoptosis gene expression and it could be used as a therapeutic agent, however, more studies must be performed to clarify its mechanism

Park City High School [9]

Exterior of Park City High School building on Park AvenueKendall Webb Collectio