Early pregnancy weight gain and fat accrual predict pregnancy outcome in growing adolescent sheep

Funding: This work was funded by the Scottish Government’s Rural and Environment Science and Analytical Services Division. Author contribution statement: JW developed the animal model, performed experiments, analysed the data and wrote the paper. RA and JM performed experiments and conducted the laboratory analysis. Acknowledgements: Dr Graham Horgan (Biomathematics and Statistics Scotland) provided statistical advice and Dr Clare Adam gifted the leptin antibody.Peer reviewedPostprin

Radio Emission from the Composite Supernova Remnant G326.3-1.8 (MSH15-56)

High resolution radio observations of the composite supernova remnant (SNR) G326.3-1.8 or MSH 15-56 with the Australia Telescope Compact Array show details of both the shell and the bright plerion which is offset about 1/3 of the distance from the center of the SNR to the shell. The shell appears to be composed of thin filaments, typical of older shell SNRs. The central part of the elongated plerion is composed of a bundle of parallel ridges which bulge out at the ends and form a distinct ring structure on the northwestern end. The magnetic field with a strength of order 45 microGauss, is directed along the axis of the ridges but circles around the northwestern ring. This plerion is large and bright in the radio but is not detected in x-ray or optical wavelengths. There is, however, a faint hard x-ray feature closer to the shell outside the plerion. Perhaps if the supernova explosion left a rapidly moving magnetar with large energy input but initially rapid decay of both relativistic particles and magnetic field, the observed differences with wavelength could be explained.Comment: 15 pages, 10 figures, accepted by Ap

Undernutrition and stage of gestation influence fetal adipose tissue gene expression

Funded by the Scottish Government’s Rural and Environment Science and Analytical Services Division (RESAS), including the Strategic Partnership for Animal Science Excellence (SPASE) and the U.S. National Institutes of Health (HD045784). None of the authors had any financial or personal conflicts of interest.Peer reviewedPostprin

The Distance to the Cygnus Loop from Hubble Space Telescope Imaging of the Primary Shock Front

We present a Hubble Space Telescope/WFPC2 narrow-band H-alpha image of a region on the northeastern limb of the Cygnus Loop supernova remnant. This location provides a detailed example of where the primary blast wave first encounters the surrounding interstellar medium. The filament structure is seen in exquisite detail in this image, which was obtained primarily as an EARLY ACQuisition image for a follow-up spectroscopic program. We compare the HST image to a digitized version of the POSS-I red plate to measure the proper motion of this filament. By combining this value for the proper motion with previous measurements of the shock velocity at this position we find that the distance to the Cygnus Loop is 440 (+130, -100) pc, considerably smaller than the canonical value of 770 pc. We briefly discuss the ramifications of this new distance estimate for our understanding of this prototypical supernova remnant.Comment: 18 pages, 3 Figures (2 JPEG and one Postscript

Perinatal complications and maximising lamb survival in an adolescent paradigm characterised by premature delivery and low birthweight

Acknowledgments Dr Anja Petrie provided supporting veterinary input in her role as Named Veterinary Surgeon. Funding acquisition: Jacqueline M. Wallace.Peer reviewedPublisher PD

Ovine prenatal growth-restriction and sex influence fetal adipose tissue phenotype and impact postnatal lipid metabolism and adiposity in vivo from birth until adulthood

Funding: This work was funded by the Scottish Government’s Rural and Environmental Science and Analytical Services Division (RESAS) including the Strategic Partnership for Animal Science. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Peer reviewedPublisher PD

From Newton's Laws to the Wheeler-DeWitt Equation

This is a pedagogical paper which explains some ideas in cosmology at a level accessible to undergraduate students. It does not use general relativity, but uses the ideas of Newtonian cosmology worked out by Milne and McCrea. The cosmological constant is also introduced within a Newtonian framework. Following standard quantization procedures the Wheeler-DeWitt equation in the minisuperspace approximation is derived for empty and non-empty universes.Comment: 13 pages, 1 figur

A 4.8- and 8.6-GHz Survey of the Large Magellanic Cloud: I The Images

Detailed 4.8- and 8.6-GHz radio images of the entire Large Magellanic Cloud with half-power beamwidths of 33" at 4.8 GHz and 20" at 8.6 GHz have been obtained using the Australia Telescope Compact Array. A total of 7085 mosaic positions were used to cover an area of 6 degrees on a side. Full polarimetric observations were made. These images have sufficient spatial resolution (~8 and 5 pc, respectively) and sensitivity (3-sigma of 1 mJy/beam) to identify most of the individual SNRs and H II regions and also, in combination with available data from the Parkes 64-m telescope, the structure of the smooth emission in that galaxy. In addition, limited data using the sixth antenna at 4.5 to 6-km baselines are available to distinguish bright point sources (<3 and 2 arcsec, respectively) and to help estimate sizes of individual sources smaller than the resolution of the full survey. The resultant database will be valuable for statistical studies and comparisons with x-ray, optical and infrared surveys of the LMC with similar resolution.Comment: 28 pages, 10 figures, 2 tables, accepted for publication in the Feb 2005 A

3D RNA-seq:A powerful and flexible tool for rapid and accurate differential expression and alternative splicing analysis of RNA-seq data for biologists

RNA-sequencing (RNA-seq) analysis of gene expression and alternative splicing should be routine and robust but is often a bottleneck for biologists because of different and complex analysis programs and reliance on specialized bioinformatics skills. We have developed the ‘3D RNA-seq’ App, an R shiny App and web-based pipeline for the comprehensive analysis of RNA-seq data from any organism. It represents an easy-to-use, flexible and powerful tool for analysis of both gene and transcript-level gene expression to identify differential gene/transcript expression, differential alternative splicing and differential transcript usage (3D) as well as isoform switching from RNA-seq data. 3D RNA-seq integrates state-of-the-art differential expression analysis tools and adopts best practice for RNA-seq analysis. The program is designed to be run by biologists with minimal bioinformatics experience (or by bioinformaticians) allowing lab scientists to analyse their RNA-seq data. It achieves this by operating through a user-friendly graphical interface which automates the data flow through the programs in the pipeline. The comprehensive analysis performed by 3D RNA-seq is extremely rapid and accurate, can handle complex experimental designs, allows user setting of statistical parameters, visualizes the results through graphics and tables, and generates publication quality figures such as heat-maps, expression profiles and GO enrichment plots. The utility of 3D RNA-seq is illustrated by analysis of data from a time-series of cold-treated Arabidopsis plants and from dexamethasone-treated male and female mouse cortex and hypothalamus data identifying dexamethasone-induced sex- and brain region-specific differential gene expression and alternative splicing

Peri- and postnatal effects of prenatal adenoviral VEGF gene therapy in growth-restricted sheep

Supported by Wellcome Trust project grant 088208 to A.L.D., J.M.W., D.M.P., I.C.Z., and J.F.M. Wellbeing of Women research training fellowship 318 to D.J.C., Scottish Government work package 4.2 to J.M.W., J.S.M., and R.P.A., as well as funding from the National Institute for Health Research University College London Hospitals Biomedical Research Centre A.L.D. and D.M.P., the British Heart Foundation to I.C.Z., and Ark Therapeutics Oy, Kuopio, Finland, which supplied adenovirus vectors free of charge.Peer reviewedPublisher PD