2,312 research outputs found
Imaging interactions between the immune and cardiovascular systems in vivo by multiphoton microscopy
Several recent studies in immunology have used multiphoton laser-scanning microscopy to visualise the induction of an immune response in real time in vivo. These experiments are illuminating the cellular and molecular interactions involved in the induction, maintenance and regulation of immune responses. Similar approaches are being applied in cardiovascular research where there is an increasing body of
evidence to support a significant role for the adaptive immune system in vascular disease. As such, we have begun to dissect the role of T lymphocytes in atherosclerosis in real time in vivo. Here, we provide step-by-step guides to the various stages involved in visualising the migration of T cells within a lymph node and their infiltration into inflamed tissues such as atherosclerotic arteries. These methods provide an insight into the mechanisms involved in the activation and function of immune cells in vivo
On variational principles for coherent vortex structures
Different approaches are discussed of variational principles characterizing coherent vortex structures in two-dimensional flows. Turbulent flows seem to form ordered structures in the large scales of the motion and the self-organization principle predicts asymptotic states realizing an extremal value of the energy or a minimum of enstrophy. On the other hand the small scales take care of the increase of entropy, and asymptotic results can be obtained by applying the theory of equilibrium statistical mechanics
Efficient Mixing at low Reynolds numbers using polymer additives
Mixing in fluids is a rapidly developing field of fluid mechanics
\cite{Sreen,Shr,War}, being an important industrial and environmental problem.
The mixing of liquids at low Reynolds numbers is usually quite weak in simple
flows, and it requires special devices to be efficient. Recently, the problem
of mixing was solved analytically for a simple case of random flow, known as
the Batchelor regime \cite{Bat,Kraich,Fal,Sig,Fouxon}. Here we demonstrate
experimentally that very viscous liquids at low Reynolds number, . Here we
show that very viscous liquids containing a small amount of high molecular
weight polymers can be mixed quite efficiently at very low Reynolds numbers,
for a simple flow in a curved channel. A polymer concentration of only 0.001%
suffices. The presence of the polymers leads to an elastic instability
\cite{LMS} and to irregular flow \cite{Ours}, with velocity spectra
corresponding to the Batchelor regime \cite{Bat,Kraich,Fal,Sig,Fouxon}. Our
detailed observations of the mixing in this regime enable us to confirm sevearl
important theoretical predictions: the probability distributions of the
concentration exhibit exponential tails \cite{Fal,Fouxon}, moments of the
distribution decay exponentially along the flow \cite{Fouxon}, and the spatial
correlation function of concentration decays logarithmically.Comment: 11 pages, 5 figure
Positive words carry less information than negative words
We show that the frequency of word use is not only determined by the word
length \cite{Zipf1935} and the average information content
\cite{Piantadosi2011}, but also by its emotional content. We have analyzed
three established lexica of affective word usage in English, German, and
Spanish, to verify that these lexica have a neutral, unbiased, emotional
content. Taking into account the frequency of word usage, we find that words
with a positive emotional content are more frequently used. This lends support
to Pollyanna hypothesis \cite{Boucher1969} that there should be a positive bias
in human expression. We also find that negative words contain more information
than positive words, as the informativeness of a word increases uniformly with
its valence decrease. Our findings support earlier conjectures about (i) the
relation between word frequency and information content, and (ii) the impact of
positive emotions on communication and social links.Comment: 16 pages, 3 figures, 3 table
Quantum Acoustics with Surface Acoustic Waves
It has recently been demonstrated that surface acoustic waves (SAWs) can
interact with superconducting qubits at the quantum level. SAW resonators in
the GHz frequency range have also been found to have low loss at temperatures
compatible with superconducting quantum circuits. These advances open up new
possibilities to use the phonon degree of freedom to carry quantum information.
In this paper, we give a description of the basic SAW components needed to
develop quantum circuits, where propagating or localized SAW-phonons are used
both to study basic physics and to manipulate quantum information. Using
phonons instead of photons offers new possibilities which make these quantum
acoustic circuits very interesting. We discuss general considerations for SAW
experiments at the quantum level and describe experiments both with SAW
resonators and with interaction between SAWs and a qubit. We also discuss
several potential future developments.Comment: 14 pages, 12 figure
Improving statistical inference on pathogen densities estimated by quantitative molecular methods: malaria gametocytaemia as a case study
BACKGROUND: Quantitative molecular methods (QMMs) such as quantitative real-time polymerase chain reaction (q-PCR), reverse-transcriptase PCR (qRT-PCR) and quantitative nucleic acid sequence-based amplification (QT-NASBA) are increasingly used to estimate pathogen density in a variety of clinical and epidemiological contexts. These methods are often classified as semi-quantitative, yet estimates of reliability or sensitivity are seldom reported. Here, a statistical framework is developed for assessing the reliability (uncertainty) of pathogen densities estimated using QMMs and the associated diagnostic sensitivity. The method is illustrated with quantification of Plasmodium falciparum gametocytaemia by QT-NASBA. RESULTS: The reliability of pathogen (e.g. gametocyte) densities, and the accompanying diagnostic sensitivity, estimated by two contrasting statistical calibration techniques, are compared; a traditional method and a mixed model Bayesian approach. The latter accounts for statistical dependence of QMM assays run under identical laboratory protocols and permits structural modelling of experimental measurements, allowing precision to vary with pathogen density. Traditional calibration cannot account for inter-assay variability arising from imperfect QMMs and generates estimates of pathogen density that have poor reliability, are variable among assays and inaccurately reflect diagnostic sensitivity. The Bayesian mixed model approach assimilates information from replica QMM assays, improving reliability and inter-assay homogeneity, providing an accurate appraisal of quantitative and diagnostic performance. CONCLUSIONS: Bayesian mixed model statistical calibration supersedes traditional techniques in the context of QMM-derived estimates of pathogen density, offering the potential to improve substantially the depth and quality of clinical and epidemiological inference for a wide variety of pathogens
Pedestrian, Crowd, and Evacuation Dynamics
This contribution describes efforts to model the behavior of individual
pedestrians and their interactions in crowds, which generate certain kinds of
self-organized patterns of motion. Moreover, this article focusses on the
dynamics of crowds in panic or evacuation situations, methods to optimize
building designs for egress, and factors potentially causing the breakdown of
orderly motion.Comment: This is a review paper. For related work see http://www.soms.ethz.c
Monitoring cardiac fibrosis: a technical challenge
The heart contains a collagen network that contributes to the contractility of the heart and provides cardiac strength. In cardiac diseases, an increase in collagen deposition is often observed. This fibrosis formation causes systolic and diastolic dysfunction, and plays a major role in the arrythmogenic substrate. Therefore, accurate detection of cardiac fibrosis and its progression is of clinical importance with regard to diagnostics and therapy for patients with cardiac disease. To evaluate cardiac collagen deposition, both invasive and non-invasive techniques are used. In this review the different techniques that are currently used in clinical and experimental setting are summarised, and the advantages and disadvantages of these techniques are discussed
Clinical examination, MRI and arthroscopy in meniscal and ligamentous knee Injuries – a prospective study
Data from 565 knee arthroscopies performed by two experienced knee surgeons between 2002 and 2005 for degenerative joint disorders, ligament injuries, loose body removals, lateral release of the patellar retinaculum, plica division, and adhesiolysis was prospectively collected. A subset of 109 patients from the above group who sequentially had clinical examination, MRI and arthroscopy for suspected meniscal and ligament injuries were considered for the present study and the data was reviewed. Patients with previous menisectomies, knee ligament repairs or reconstructions and knee arthroscopies were excluded from the study. Patients were categorised into three groups on objective clinical assessment: Those who were positive for either meniscal or cruciate ligament injury [group 1]; both meniscal and cruciate ligament injury [group 2] and those with highly suggestive symptoms and with negative clinical signs [group 3]. MRI was requested for confirmation of diagnosis and for additional information in all these patients. Two experienced radiologists reported MRI films. Clinical and MRI findings were compared with Arthroscopy as the gold standard. A thorough clinical examination performed by a skilled examiner more accurately correlated at Arthroscopy. MRI added no information in group 1 patients, valuable information in group 2 and was equivocal in group 3 patients. A negative MRI did not prevent an arthroscopy. In this study, specificity, positive and negative predictive values were more favourable for clinical examination though MRI was more sensitive for meniscal injuries. The use of MRI as a supplemental tool in the management of meniscal and ligament injuries should be highly individualised by an experienced surgeon
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Correction: Precision weighting of cortical unsigned prediction error signals benefits learning, is mediated by dopamine, and is impaired in psychosis.
An amendment to this paper has been published and can be accessed via a link at the top of the paper.</jats:p
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