Acid pH activation of the PmrA/PmrB two-component regulatory system of Salmonella enterica

Acid pH often triggers changes in gene expression. However, little is known about the identity of the gene products that sense fluctuations in extracytoplasmic pH. The Gram-negative pathogen Salmonella enterica serovar Typhimurium experiences a number of acidic environments both inside and outside animal hosts. Growth in mild acid (pH 5.8) promotes transcription of genes activated by the response regulator PmrA, but the signalling pathway(s) that mediates this response has thus far remained unexplored. Here we report that this activation requires both PmrA's cognate sensor kinase PmrB, which had been previously shown to respond to Fe(3+) and Al(3+), and PmrA's post-translational activator PmrD. Substitution of a conserved histidine or of either one of four conserved glutamic acid residues in the periplasmic domain of PmrB severely decreased or abolished the mild acid-promoted transcription of PmrA-activated genes. The PmrA/PmrB system controls lipopolysaccharide modifications mediating resistance to the antibiotic polymyxin B. Wild-type Salmonella grown at pH 5.8 were > 100 000-fold more resistant to polymyxin B than organisms grown at pH 7.7. Our results suggest that protonation of the PmrB periplasmic histidine and/or of the glutamic acid residues activate the PmrA protein, and that mild acid promotes cellular changes resulting in polymyxin B resistance

Influence of temperature gradients on tunnel junction thermometry below 1 K: cooling and electron-phonon coupling

We have studied thermal gradients in thin Cu and AlMn wires, both experimentally and theoretically. In the experiments, the wires were Joule heated non-uniformly at sub-Kelvin temperatures, and the resulting temperature gradients were measured using normal metal-insulator-superconducting tunnel junctions. The data clearly shows that even in reasonably well conducting thin wires with a short ($\sim 10 \mu$m) non-heated portion, significant temperature differences can form. In most cases, the measurements agree well with a model which includes electron-phonon interaction and electronic thermal conductivity by the Wiedemann-Franz law.Comment: J. Low Temp. Phys. in pres

Generalized Statistics and High Tc Superconductivity

Introducing the generalized, non-extensive statistics proposed by Tsallis[1988], into the standard s-wave pairing BCS theory of superconductivity in 2D yields a reasonable description of many of the main properties of high temperature superconductors, provided some allowance is made for non-phonon mediated interactions.Comment: 14 pages, 5 figure

Nitrosative stress treatment of E. coli targets distinct set of thiol-containing proteins

Reactive nitrogen species (RNS) function as powerful antimicrobials in host defence, but so far little is known about their bacterial targets. In this study, we set out to identify Escherichia coli proteins with RNS-sensitive cysteines. We found that only a very select set of proteins contain cysteines that undergo reversible thiol modifications upon nitric oxide (NO) treatment in vivo . Of the 10 proteins that we identified, six (AtpA, AceF, FabB, GapA, IlvC, TufA) have been shown to harbour functionally important thiol groups and are encoded by genes that are considered essential under our growth conditions. Media supplementation studies suggested that inactivation of AceF and IlvC is, in part, responsible for the observed NO-induced growth inhibition, indicating that RNS-mediated modifications play important physiological roles. Interestingly, the majority of RNS-sensitive E. coli proteins differ from E. coli proteins that harbour H 2 O 2 -sensitive thiol groups, implying that reactive oxygen and nitrogen species affect distinct physiological processes in bacteria. We confirmed this specificity by analysing the activity of one of our target proteins, the small subunit of glutamate synthase. In vivo and in vitro activity studies confirmed that glutamate synthase rapidly inactivates upon NO treatment but is resistant towards other oxidative stressors.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/72397/1/j.1365-2958.2007.05964.x.pd

Network patterns and strength of orbital currents in layered cuprates

In a frame of the $t-J-G$ model we derive the microscopical expression for the circulating orbital currents in layered cuprates using the anomalous correlation functions. In agreement with $\mu$-on spin relaxation ($\mu$SR), nuclear quadrupolar resonance (NQR) and inelastic neutron scattering(INS) experiments in YBa$_2$Cu$_3$O$_{6+x}$ we successfully explain the order of magnitude and the monotonous increase of the {\it internal} magnetic fields resulting from these currents upon cooling. However, the jump in the intensity of the magnetic fields at T$_c$ reported recently seems to indicate a non-mean-field feature in the coexistence of current and superconducting states and the deviation of the extended charge density wave vector instability from its commensurate value {\bf Q}$\approx(\pi,\pi$) in accordance with the reported topology of the Fermi surface

New calculations of the PNC Matrix Element for the JπTJ^{\pi}T 0+1,0−1^{+}1,0^{-}1 doublet in 14^{14}N

A new calculation of the predominantly isoscalar PNC matrix element between the $J^{\pi}T$ $0^{+}1,0^{-}1$ (E$_{x} \approx$ 8.7 MeV) states in $^{14}$N has been carried out in a (0+1+2+3+4)$\hbar \omega$ model space with the Warburton-Brown interaction. The magnitude of the PNC matrix element of 0.22 to 0.34 eV obtained with the DDH PNC interaction is substantially suppressed compared with previous calculations in smaller model spaces but shows agreement with the preliminary Seattle experimental data. The calculated sign is opposite to that obtained experimentally, and the implications of this are discussed.Comment: REVTEX, 28 page

Cosmic Chronometers: Constraining the Equation of State of Dark Energy. I: H(z) Measurements

We present new determinations of the cosmic expansion history from red-envelope galaxies. We have obtained for this purpose high-quality spectra with the Keck-LRIS spectrograph of red-envelope galaxies in 24 galaxy clusters in the redshift range 0.2 < z < 1.0. We complement these Keck spectra with high-quality, publicly available archival spectra from the SPICES and VVDS surveys. We improve over our previous expansion history measurements in Simon et al. (2005) by providing two new determinations of the expansion history: H(z) = 97 +- 62 km/sec/Mpc at z = 0.5 and H(z) = 90 +- 40 km/sec/Mpc at z = 0.8. We discuss the uncertainty in the expansion history determination that arises from uncertainties in the synthetic stellar-population models. We then use these new measurements in concert with cosmic-microwave-background (CMB) measurements to constrain cosmological parameters, with a special emphasis on dark-energy parameters and constraints to the curvature. In particular, we demonstrate the usefulness of direct H(z) measurements by constraining the dark- energy equation of state parameterized by w0 and wa and allowing for arbitrary curvature. Further, we also constrain, using only CMB and H(z) data, the number of relativistic degrees of freedom to be 4 +- 0.5 and their total mass to be < 0.2 eV, both at 1-sigma.Comment: Submitted to JCA