Distinct forebrain and cerebellar isozymes of type II Ca^(2+)/calmodulin-dependent protein kinase associate differently with the postsynaptic density fraction

Forebrain and cerebellar Type II Ca2+/calmodulin-dependent protein kinases have different subunit compositions. The forebrain holoenzyme, characterized in our laboratory, is a 650-kDa holoenzyme composed of 50-kDa alpha-subunits and 60-kDa beta-subunits assembled in approximately a 3:1 ratio (Bennett, M. K., Erondu, N. E., and Kennedy, M. B. (1983) J. Biol. Chem. 258, 12735-12744). The cerebellar isozyme is a 500-kDa holoenzyme composed of alpha-subunits and beta-subunits assembled in almost the converse ratio, approximately four beta-subunits for each alpha-subunit. When compared by tryptic peptide mapping and by immunochemical techniques, the beta-subunits from the two brain regions are indistinguishable and the alpha-subunits appear closely related. The specific activities, substrate specificities, and catalytic constants of the cerebellar and forebrain isozymes are similar, suggesting that the alpha- and beta-subunits contain similar catalytic sites. However, two differences in the properties of the isozymes may result in functional differences between them in vivo. First, the apparent affinity of the cerebellar kinase for Ca2+/calmodulin is 2-fold higher than that of the forebrain kinase. Second, the two isozymes appear to associate differently with subcellular structures. Approximately 85% of the cerebellar kinase and 50% of the forebrain kinase remain in the particulate fraction after homogenization under standard conditions. However, they are present in different amounts in postsynaptic density fractions. Postsynaptic densities prepared from forebrain contain the forebrain isozyme. Immunochemical measurements show that it comprises approximately 16% of their total protein. In contrast, postsynaptic densities prepared from cerebellum contain the cerebellar isozyme, but it comprises only approximately 1-2% of their total protein. Thus, the alpha-subunit may play a role in anchoring Type II Ca2+/calmodulin-dependent protein kinase to postsynaptic densities

Recent Radar Observations of the Sub-Centimeter Orbital Debris Environment

The NASA Orbital Debris Program Office (ODPO) has conducted radar observations of the orbital debris environment since the early 1990s to provide measurement data that supports orbital debris models and risk mitigation activities in support of NASA mission objectives. Orbital debris radar observations are a unique mode for radar operation, employing a fixed beam configuration to statistically sample the environment. An advantage of conducting operations in this fashion is that it enables observations of smaller classes of orbital debris than would otherwise be available from the same sensor operating in a traditional tracking mode. Orbital debris-mode radar observations are used to fill in the gaps, which exist in the currently available data from the Space Surveillance Network (SSN), on small size orbital debris populations that represent significant risk to NASA programs. These gaps have typically covered orbital debris with characteristic sizes less than approximately 10 cm down to approximately 3 mm in low Earth orbit (LEO) depending upon the altitude and sensor configuration. The value of orbital debris radar measurements lies in the ability to extract partial orbital element information about orbital debris in the centimeter to several millimeter size regimes in low Earth orbit which are not available from other measurement sources. This paper will discuss observations of this smaller class of orbital debris observed in recent years from the radars at the MIT Haystack Observatory in Westford, Massachusetts, and the Goldstone Solar System Radar near Barstow, California. The former radar is able to observe orbital debris down to approximately 5 mm, and the latter, orbital debris with characteristic sizes near 3 mm at altitudes less than 1000 km. The characteristics and inferences about the current LEO orbital debris environment, and the different subpopulations that are identifiable in the observations are highlighted

Activation of type II calcium/calmodulin-dependent protein kinase by Ca^(2+)/calmodulin is inhibited by autophosphorylation of threonine within the calmodulin-binding domain

It is now well established that autophosphorylation of a threonine residue located next to each calmodulin-binding domain in the subunits of type II Ca^(2+)/calmodulin-dependent protein kinase causes the kinase to remain active, although at a reduced rate, after Ca^(2+) is removed from the reaction. This autophosphorylated form of the kinase is still sensitive to Ca2+/calmodulin, which is required for a maximum catalytic rate. After removal of Ca^(2+), new sites are autophosphorylated by the partially active kinase. Autophosphorylation of these sites abolishes sensitivity of the kinase to Ca^(2+)/calmodulin (Hashimoto, Y., Schworer, C. M., Colbran, R. J., and Soderling, T. R. (1987) J. Biol. Chem. 262, 8051-8055). We have identified two pairs of homologous residues, Thr^(305) and Ser^(314) in the alpha subunit and Thr^(306) and Ser^(315) in the beta subunit, that are autophosphorylated only after removal of Ca^(2+) from an autophosphorylation reaction. The sites were identified by direct sequencing of labeled tryptic phosphopeptides isolated by reverse-phase high pressure liquid chromatography. Thr^(305-306) is rapidly dephosphorylated by purified protein phosphatases 1 and 2A, whereas Ser^(314-315) is resistant to dephosphorylation. We have shown by selective dephosphorylation that the presence of phosphate on Thr^(305-306) blocks sensitivity of the kinase to Ca^(2+)/calmodulin. In contrast, the presence of phosphate on Ser^(314-315) is associated with an increase in the Kact for Ca^(2+)/calmodulin of only about 2-fold, producing a relatively small decrease in sensitivity to Ca^(2+)/calmodulin

Orbital Debris Radar Measurements from the Haystack Ultra-Wideband Satellite Imaging Radar (HUSIR): 2014-2017

For many years, the NASA Orbital Debris Program Office (ODPO) has partnered with the U.S. Department of Defense and the Massachusetts Institute of Technology Lincoln Laboratory (MIT/LL) to collect data on the orbital debris environment using the Haystack radar. These measurements are used to characterize the small debris environment in low Earth orbit (LEO), down to a noise-limited size of approximately 5 mmdepending on altitude. The Haystack radar operated by MIT Lincoln Lab underwent upgrades starting in May 2010, with operations resuming in 2014 as the Haystack Ultra-wideband Satellite Imaging Radar (HUSIR). Hence, the data collected beginning in 2014 represents the first dataset available from this upgraded sensor. HUSIR is the primary source of data used by the ODPO to statistically sample orbital debris in the 5-mm to 10-cm size regime in LEO and is a key source of data to build and validate the NASA Orbital Debris Engineering Model. In this paper, we will present recent results from measurements performed during the US Government fiscal years 2014 2017. Using the NASA Size Estimation Model, a method based on laboratory radar measurements of debris, we will compare the size distributions of selected orbital debris populations over this 4-year period and flux measurements of orbital debris greater than 1 cm

Student and Teacher Perceptions of Multiliterate Assignments Utilizing 21st Century Skills

Today’s society requires students to be knowledgeable in both content and skill to be successful. In the secondary classroom it is important to fully prepare students for their futures in the post-secondary classroom or for their career, and through the implementation of Common Core State Standards, this focus has been emphasized in educational pedagogy. This thesis outlines a study and the implications of the perceptions of teachers and students on utilizing 21st century skills in the secondary English classroom through the implementation of multiliterate assignments. This thesis outlines reasons for the study, important terminology to ground the study, the methodology, the results, and conclusions of the study. This research was designed to understand: how student and teachers believe multiliterate assignments impact students’ ability to utilize 21st century skills; how those 21st century skills are present in multiliterate assignments; and what effects the use of 21st century skills have on the mastery of course content. The study focuses on a qualitative approach to analyzing if teachers and students believe that multiliterate assignments help to facilitate the use of the 21st century skills of communication, creativity, problem-solving, and critical thinking. Through surveys and reflection data collection, teachers and students indicated that multiliterate assignments do facilitate the use of the four main 21st century skills that are needed for college, career, and life. This research indicates that utilizing multiliterate assignments in the English classroom can benefit students in preparing for life outside of high school

The NaK Population: A 2019 Status

The statistical debris measurement campaigns conducted by the Haystack Ultrawideband Satellite Imaging Radar (HUSIR) on behalf of the NASA Orbital Debris Program Office are used to characterize the long-term behavior of the small, low Earth orbit (LEO) orbital debris environment. A long-recognized, unique component of the LEO environment is composed of small Sodium-Potassium (NaK) eutectic nuclear reactor coolant droplets associated with the Soviet Radar Ocean Reconnaissance Satellite (RORSAT) program. Beginning with the flight of Cosmos 1176, RORSAT vehicles would nominally separate their reactor core at end of mission, thereby venting the NaK coolant and producing the NaK droplet population. In this paper, we describe the methodology by which NaK are segregated from the statistically sampled general debris population and their sizes inferred; the current NaK environment; how it has changed over time; and a potential new source of NaK: RORSAT vehicles that did not separate their reactor core by either design or apparent malfunction

Recent Results from the Goldstone Orbital Debris Radar: 2016-2017

Since 1993, the NASA Orbital Debris Program Office has used the Goldstone Orbital Debris Radar (Goldstone) to sample statistically the orbital debris environment. Due to the sensitivity of this radar, which can detect an approximately 3 mm-diameter conducting sphere at 1,000 km, it has filled an important role in the characterization of the sub-centimeter-sized orbital debris population. Through the years, the capabilities of this system have increased recent updates include increased receiver bandwidth and a change in the bi-static observation geometry both of which enhance the radars ability to estimate orbital parameters. In 2016, dual polarization capability was added, making this the first year where both right- and left-hand circularly polarized information was available from this sensor. This additional polarization information may enable better characterization of sub-centimeter-sized particles in low Earth orbit, particularly since the receiver triggers on reflected energy from both left- and right-handed circular polarizations independently. In this paper, we present measurements and results derived from data taken during the calendar years (CY) 2016-2017 by Goldstone and compare this dataset to measurements taken by the Haystack Ultra-wideband Satellite Imaging Radar (HUSIR) during a similar timeframe

The effect of water immersion on perception of the oculogravic illusion in normal and labyrinthine-defective subjects

Perception of oculogravic illusion in normal and labyrinthine-defective subjects related to water immersio

A predicted dimer-based polymorph of 10,11-dihydrocarbamazepine (Form IV)

A novel polymorph of 10,11-dihydrocarbamazepine (form IV), which had been predicted to be thermodynamically feasible, was obtained from the vapour phase and displays an R22(8) hydrogen bonded dimer motif in contrast to the catemeric motifs in forms I–III