Varijabilnost agronomskih osobina hibrida kukuruza pod uticajem faktora sredine

In this study 36 maize hybrids of different FAO maturity groups were observed in three successive years (2011, 2012 and 2013), on 8 locations. The main objective of this experiment was to observe the GxE interaction concerning yield, grain moisture, grain yield per ear and test weight. The experiment was set up according to the RCBD. Based on the obtained results average estimates, CV and overall ranking of hybrids were calculated. ANOVA was applied in order to estimate the effect of factors: genotype, environment and interaction. Thus the significance of all these factors was observed. Results of this research indicate the importance and necessity of performing multilocation and multiyear trials with the aim of observation and understanding the intensity of GxE interaction, as well as its influence on the grain yield and it components.Istraživanje je obuhvatilo 36 hibrida različitih FAO grupa zrenja, u 2011, 2012 i 2013 godini, na 8 lokaliteta. U ogledu je ispitivana interakcija genotip x sredina u pogledu stabilnosti prinosa zrna, vlage zrna, težine zrna po klipu i zapreminske mase kukuruza. Ogled je bio postavljen potpuno slučajnom blok sistemu (RCBD). Na osnovu dobijenih podataka izračunate su prosečne vrednosti, pokazatelji varijabilnosti posmatranih osobina i ukupan rang hibrida, a analizom varijanse utvrđena je značajnost efekta genotipa, sredina i interakcija. Za sve proučavane agronomske osobine, analizom varijanse su utvrđene statistički visokoznačajne vrednosti genotipa, sredina i interakcije. Najviši prosečan prinos zrna u ogledu ostvaren je u 2011. godini (11,62 t/ha), a najniži u 2012. godini (6,90 t/ha). Najniži prosečan procenat vlage zrna kukuruza u ogledu ostvaren je u veoma sušnoj 2012. godini (14,86%), dok između vrednosti ostvarenih u 2011. godini (19,47%) i 2013. godini (19,52%) nije bilo značajnije razlike. Vrednosti težine zrna po klipu kukuruza bile su direktno srazmerne vrednostima prinosa. Najveća zapreminska masa zrna kukuruza ostvarena je u sušnoj 2012. godini (74,84 kg/hl), dok je najmanje izmerena u kišovitoj 2013. godini (70,47 kg/hl). Rezultati ovog istraživanja potvrđuju neophodnost izvođenja višelokacijskih i višegodišnjih ogleda u cilju što boljeg sagledavanja intenziteta interakcija genotip x sredina, i njihovog uticaja na prinos i komponente prinosa hibrida kukuruza različitih FAO grupa zrenja na teritoriji Srbije. Da bi proizvodnja kukuruza bila stabilna, farmerima koji nemaju mogućnost navodnjavanja, već kukuruz gaje u suvom ratarenju, treba savetovati da seju hibride različite dužine vegetacije

EP12.075 Detection rate of 22q11.2 microdeletion using strict diagnostic criteria

Background/Objectives: 22q11.2 microdeletion, detected in patients with 22q11.2 Deletion Syndrome (22q11.2DS), is the most common microdeletion syndrome in humans. 22q11.2DS has high risk for neurodevelopmental disorders and is associated with more than 180 malformations. Many investigations of the 22q11.2 microdeletion applying different recruitment criteria, revealed detection rate ranging from zero to 34.7%. Here we analyzed the frequency of 22q11.2 microdeletion among children having at least two out of five major characteristics of 22q11.2DS: congenital heart malformations (CHM), facial dysmorphism, immunological problems, palatal clefts and hypocalcemia. Methods: Children with clinical characteristics of 22q11.2DS were analyzed. Fluorescence in situ hybridization and multiplex ligation-dependent probe amplification analysis were applied for detection of 22q11.2 microdeletion. Results: 22q11.2 microdeletion was detected in approximately 40% of children. CHM was found in all patients with 22q11.2 microdeletion. Dysmorphic facial features were present in about 45%, immunological problems in 30%, overt cleft palate in about 4% and hypocalcemia in approximately 60% of patients with 22q11.2 microdeletion. Conclusion: When at least two major features of 22q11.2DS are taking into consideration higher detection rate is obtained compared to one-feature criterion. These criteria could be considered by centers in low-income countries.Abstracts from the 55th European Society of Human Genetics (ESHG) Conference: e-Poster

Effect of chemical and hand thinning young apple tree on yield and fruit quality

Abstract The aim of this study was to evaluate the effectiveness of two thinning agents in relation to hand thinning in reducing fruit set in 'Gala' and 'Granny Smith' young apple trees. The agents tested were NAA (two concentrations) and carbaryl. Chemical and hand thinning showed greater influence on reduction fruit set in cv. 'Granny Smith' than in cv. 'Gala'. Fruit thinning significantly increased the average fruit size, especially in cv. 'Granny Smith'. In cv. 'Gala', similar effect was only observed in treatment with higher concentration of NAA. Fruit thinning did not influence on other fruit quality parameters for both cultivars. A high return bloom was recorded in treatments with thinning fruit in comparison to unsprayed treatment. It was higher in cv. 'Granny Smith' than cv. 'Gala&apos

Karakteristike izolata Botrytis cinerea iz jagodastih voćaka i vinove loze poreklom iz Srbije

Twenty-six single-spore isolates of Botrytis cinerea from blackberry, raspberry, strawberry, and grapevine were investigated using transposable elements, morphological characterization, and sensitivity to fungicides. Both transposable elements, Flipper and Boty, were detected among isolates from all the hosts. Six vacuma (without transposable elements) and seven transposa (containing both elements) isolates were found to be present in sympatry in Serbia. Isolates containing only the Boty element were detected. Eight morphological types of colonies on PDA and MA media were observed, confirming the great phenotypic variability of B. cinerea. Sensitivity to fungicides was various, depending on both the fungicide and the isolate.U radu su proučene morfološke karakteristike, prisustvo transpozona u genomu i osetljivost na fungicide 26 monosporijalnih izolata Botrytis cinerea, izolovanih iz kupine, maline, jagode i vinove loze. Prisustvo oba transpozona, Flipper i Boty, utvrđeno je u izolatima iz svih domaćina. Detektovano je šest vacuma (ne sadrže transpozone) i sedam transposa (sadrže oba transpozona) izolata, kao i 13 izolata koji sadrže samo transpozon Boty. Zapaženo je osam morfoloških tipova kolonija na PDA i MA podlozi, što potvrđuje poznatu visoku varijabilnost vrste B. cinerea. Osetljivost na fungicide je bila različita, zavisno od fungicida i izolata

Growing primocane raspberry cultivars in Serbia

In this paper, first results from trials with primocane fruiting raspberry cultivars 'Autumn Bliss', 'Golden Bliss', 'Polana' and 'Polka' are shown. These cultivars are grown on relative small plots in Serbia. Previous results has shown that mentioned cultivars expressed good fruit properties with regard fruit mass and content of soluble solids. It can be explained with the favourable growing condition for raspberries in Serbia. The best results were obtained with red fruited cultivar Polana and the yellow fruited cultivar 'Golden Bliss'

GROWING PRIMOCANE RASPBERRY CULTIVARS IN SERBIA

In this paper, first results from trials with primocane fruiting raspberry cultivars 'Autumn Bliss', 'Golden Bliss', 'Polana' and 'Polka' are shown. These cultivars are grown on relative small plots in Serbia. Previous results has shown that mentioned cultivars expressed good fruit properties with regard fruit mass and content of soluble solids. It can be explained with the favourable growing condition for raspberries in Serbia. The best results were obtained with red fruited cultivar Polana and the yellow fruited cultivar 'Golden Bliss'

Primary lymphoma of the brain in a young man whose brother died of hemophagocytic lymphohistiocytosis: Case report

Introduction. We represent the unique occurrence of primary central nervous system lymphoma (PCNSL) in a patient whose brother died of genetically confirmed hemophagocytic lymphohistiocytosis (HLH). Case Outline. We report a case of a 25-year-old male patient with primary aggressive diffuse large B-cell lymphoma affecting the brain and PCNSL. Despite one year of medical treatment outcome was lethal. However, our patient had a relatively longer survival compared to median survival time for PCNSL. Additionally, he had two older brothers who died at the age of about 11 years. One died of fulminate malignancy, shortly after pediatric admission, before the diagnosis could be established. The other one died from genetically confirmed (perforin mutation/PRF1) HLH. Our patient was heterozygous carrier of perforin mutation representing the genetic marker for HLH. Our patient’s father was the carrier of the same mutation but had no symptoms of any disease. Conclusion. This case points at the presence of HLH and diffuse large B-cell PCNSL in brothers. Extensive assessment of patients with probable PCNSL and familial HLH is necessary, including genetic analysis for HLH. [Projekat Ministarstva nauke Republike Srbije, br. 175090

The role of modern imaging techniques in the diagnosis of malposition of the branch pulmonary arteries and possible association with microdeletion 22q11.2

Malposition of the branch pulmonary arteries is a rare malformation with two forms. In the typical form, pulmonary arteries cross each other as they proceed to their respective lungs. The "lesser form" is characterised by the left pulmonary artery ostium lying directly superior to the ostium of the right pulmonary artery, without crossing of the branch pulmonary arteries. Malposition of the branch pulmonary arteries is often associated with other congenital heart defects and extracardiac anomalies, as well as with 22q11.2 microdeletion. We report three infants with crossed pulmonary arteries and one adolescent with "lesser form" of the malformation. The results suggest that diagnosis of malposition of the branch pulmonary arteries could be challenging if based solely on echocardiography, whereas modern imaging technologies such as contrast computed tomography and magnetic resonance angiography provide reliable establishment of diagnosis. In addition, we performed the first molecular characterisation of the 22q11.2 region among patients with malposition of the branch pulmonary arteries and revealed a 3-megabase deletion in two out of four patients

Improving the Diagnosis of Children with 22q11.2 Deletion Syndrome: A Single-center Experience from Serbia

Objective: The incidence of the 22q11.2 microdeletion among children who have at least two out of five major clinical criteria for 22q11.2 deletion syndrome. Design: Prospective study. Setting: University Children's Hospital in Belgrade, Serbia between 2005 and 2014. Participants: 57 patients with clinical characteristics of 22q11.2 deletion syndrome. Methods: Standard G-banding cytogenetic analysis was performed in all children, and the 22q11.2 genomic region was examined using fluorescence in situ hybridization (FISH). For patients with no deletion detected by FISH, multiplex ligation dependent probe amplification (MLPA) analysis was also done in order to detect cryptic deletions of this region and to analyze other genomic loci associated with phenotypes resembling the syndrome. A selected group of patients diagnosed to have 22q11.2 microdeletion by FISH underwent MLPA testing in order to characterize the size and position of deletion. Outcome Measure: The frequency of 22q11.2 microdeletion among children with at least two of the five major characteristics of 22q11.2 deletion syndrome (heart malformations, facial dysmorphism, T-cell immunodeficiency, palatal clefts and hypocalcemia/hypoparathyroid ism) Results: Typical 22q11.2 microdeletion was detected in 42.1% of patients; heart malformation were identified in all of them, facial dysmorphism in 79.2%, immunological problems in 63.6%, hypocalcemia in 62.5% and cleft palate in 8.3%. Conclusions: A higher detection rate compared to one-feature criterion is obtained when at least two major features of 22q11.2 deletion syndrome are taking into consideration. The criteria applied in this study could be considered by centers in low-income countries

Genetic evaluation of newborns with critical congenital heart defects admitted to the intensive care unit

Rapid and efficient diagnostics is crucial for newborns with congenital heart defects (CHD) in intensive care unit (ICU) but is often challenging. Given that genetic factors play a role in 20-30% cases of CHD, it is likely that genetic tests could improve both its speed and efficiency. We aimed to analyze the utility of rapid and cost-effective multiplex ligation dependent probe amplification analysis (MLPA) for chromosomal analysis in newborns with critical CHD. One hundred consecutive newborns admitted with critical CHD to the ICU were included in the study. Those with normal MLPA findings were further tested by chromosomal microarray and clinical exome sequencing. Overall, pathogenic/likely pathogenic variants were determined in ten (10%) newborns by MLPA, three (3%) by chromosomal microarray, and three (3%) by clinical exome sequencing. The most common variant detected was deletion of 22q11.2 region. Conclusion: MLPA is fast and cost-effective analysis that could be used as the first-tier test in newborns with critical CHD admitted to the ICU. What is Known: center dot MLPA is an established method for chromosome analysis in patients with CHD, but detection rate in newborns with critical CHD is unknown. What is New: center dot Study suggests that detection rate of casual variants using MLPA in newborns with critical CHD is 10%