2 research outputs found
Orthorexia nervosa, mindful eating, and perfectionism: an exploratory investigation
Purpose
Research has drawn associations between Mindful Eating (ME) and perfectionism in the aetiology and treatment of eating disorders (ED), but understanding into the relationship between these factors and Orthorexia nervosa (ON) is limited. The purpose of this research is to explore the relationship between perfectionism, ME, and ON.
Method
Participants (nâ=â670) completed the DĂŒsseldorf Orthorexia scale, the Mindful Eating Behavior scale, and the Big-Three Perfectionism scale Short-form, to reveal the relationship between ON, ME, and perfectionism. The relationship was assessed using correlational and regression analyses.
Results
A positive association was observed between perfectionism and ON. Moreover, perfectionism demonstrated a significant negative correlation with three out of four ME facets, with âeating without distractionâ displaying the highest correlation. The âeating with awarenessâ facet of ME demonstrated a significant relationship with ON, in a negative direction. An unexpected relationship was observed between the focused eating facet of ME and ON, with a positive association being found. A further regression analysis revealed both perfectionism and ME to predict orthorexic tendencies.
Conclusion
These findings identify a relationship between ON, ME, and perfectionism. It offers suggestion for the complexity of ME, and how it should be recognised by its different components, estimating a differential predictability and estimation of ON. Further research is required to clarify the direction of causality in the relationships observed, to inform the clinical diagnoses and intervention of ON.
Level of evidence
Level V, cross-sectional descriptive study
Optimization of Surface Display of DENV2 E Protein on a Nanoparticle to Induce Virus Specific Neutralizing Antibody Responses
The
dengue virus (DENV) causes over 350 million infections, resulting
in âŒ25,000 deaths per year globally. An effective dengue vaccine
requires generation of strong and balanced neutralizing antibodies
against all four antigenically distinct serotypes of DENV. The leading
live-attenuated tetravalent dengue virus vaccine platform has shown
partial efficacy, with an unbalanced response across the four serotypes
in clinical trials. DENV subunit vaccine platforms are being developed
because they provide a strong safety profile and are expected to avoid
the unbalanced immunization issues associated with live multivalent
vaccines. Subunit vaccines often lack immunogenicity, requiring either
a particulate or adjuvanted formulation. Particulate formulations
adsorbing monomeric DENV-E antigen to the particle surface incite
a strong immune response, but have no control of antigen presentation.
Highly neutralizing epitopes are displayed by DENV-E quaternary structures.
To control the display of DENV-E and produce quaternary structures,
particulate formulations that covalently attach DENV-E to the particle
surface are needed. Here we develop a surface attached DENV2-E particulate
formulation, as well as analysis tools, using PEG hydrogel nanoparticles
created with particle replication in nonwetting templates (PRINT)
technology. We found that adding Tween-20 to the conjugation buffer
controls DENV-E adsorption to the particle surface during conjugation,
improving both protein stability and epitope display. Immunizations
with the anionic but not the cationic DENV2-E conjugated particles
were able to produce DENV-specific and virus neutralizing antibody
in mice. This work optimized the display of DENV-E conjugated to the
surface of a nanoparticle through EDC/NHS chemistry, establishing
a platform that can be expanded upon in future work to fully control
the display of DENV-E