Additional file 4 of TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy

Additional file 4. Individual data points

Additional file 5 of TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy

Additional file 5. Summary of statistics

Additional file 2 of TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy

Additional file 2. Patched neurons in the hilus are SATB1 positive. Representative confocal images of biocytinfilled WTand Trpm4−/−MCs counterstained with SATB1. Note that the biocytin filled cells are also SATB1 positive. Scale bar 5 μm. Image of WT neuronwas modified from previous publication [6]

Additional file 3 of TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy

Additional file 3. Power spectrum of epileptic WT and Trpm4−/− mice is not different.Power spectral density plot of epileptic WTand Trpm4−/−mice during exploration.Statistics showing theta, alpha, betaand gammapower in epileptic WTand Trpm4−/−mice. n = 6 for WT and 6 for Trpm4−/− mice

Additional file 1 of TRPM4 regulates hilar mossy cell loss in temporal lobe epilepsy

Additional file 1. SATB1 and Glur2/3 are colocalized in the hilus. Representative 10xand 60xconfocal images of double immunofluorescence staining for SATB1and GLuR2/3.Left, percentage of SATB1 positive neurons that express GLuR2/3. Right, percentage of GLuR2/3 positive neurons that express SATB1. n = 211 neurons from 2 mice. Scale bar 100 μmand 5 μm