Neuronal precursor cell proliferation in the hippocampus after transient cerebral ischemia: a comparative study of two rat strains using stereological tools

BACKGROUND: We are currently investigating microglial activation and neuronal precursor cell (NPC) proliferation after transient middle cerebral artery occlusion (tMCAo) in rats. This study aimed: (1) to investigate differences in hippocampal NPC proliferation in outbred male spontaneously hypertensive rats (SHRs) and Sprague-Dawley rats (SDs) one week after tMCAo; (2) to present the practical use of the optical fractionator and 2D nucleator in stereological brain tissue analyses; and (3) to report our experiences with an intraluminal tMCAo model where the occluding filament is advanced 22 mm beyond the carotid bifurcation and the common carotid artery is clamped during tMCAo. METHODS: Twenty-three SDs and twenty SHRs were randomized into four groups subjected to 90 minutes tMCAo or sham. BrdU (50 mg/kg) was administered intraperitoneally twice daily on Day 4 to 7 after surgery. On Day 8 all animals were euthanized. NeuN-stained tissue sections were used for brain and infarct volume estimation with the 2D nucleator and Cavalieri principle. Brains were studied for the presence of activated microglia (ED-1) and hippocampal BrdU incorporation using the optical fractionator. RESULTS: We found no significant difference or increase in post-ischemic NPC proliferation between the two strains. However, the response to remote ischemia may differ between SDs and SHRs. In three animals increased post-stroke NPC proliferation was associated with hippocampal ischemic injury. The mean infarct volume was 89.2 ± 76.1 mm(3 )in SHRs and 16.9 ± 22.7 mm(3 )in SDs (p < 0.005). Eight out of eleven SHRs had ischemic neocortical damage in contrast to only one out of 12 SDs. We observed involvement of the anterior choroidal and hypothalamic arteries in several animals from both strains and the anterior cerebral artery in two SHRs. CONCLUSIONS: We found no evidence of an early hippocampal NPC proliferation one week after tMCAo in both strains. Infarction within the anterior choroidal artery could induce hippocampal ischemia and increase NPC proliferation profoundly. NPC proliferation was not aggravated by the presence of activated microglia. Intraluminal tMCAo in SHRs gave a more reliable infarct with neocortical involvement, but affected territories supplied by the anterior cerebral, anterior choroidal and hypothalamic arteries

Comparison of WLAN Probe and Light Sensor Based Estimators of Bus Occupancy Using Live Deployment Data

Bus company operators are interested in obtaining knowledge about the number of passengers on their buses—preferably doing so at low deployment costs and in an automated manner, while keeping accuracy high. One solution, widely used in practice, involves deploying a light sensor-based system, counting the people entering and leaving the bus. The light sensor system is simple, but errors accumulate over time, because it is not capable of error correcting. For this reason, the light sensor-based system is compared to a WLAN probe-based system, which has entirely different characteristics. Inaccuracy with the WLAN estimator comes from a need to filter out mobile devices outside the bus and to map the number of detected devices to a number of people. The comparison is performed based on data collected from a real-life deployment in a medium sized German city. The comparison shows the trade-off in selecting either of the two methods. Furthermore, a novel approach for fusion of the light sensor and WLAN estimators is proposed which has a big potential in improving accuracy of both estimators. A fusion approach is proposed that utilizes the different error characteristics for error compensation by calculating compensation terms. The knowledge of Ground Truth is not required as part of this fusion approach for calibration; results show that the approach can find the optimal parameter settings and that it makes this occupancy estimation approach scalable and automated