14 research outputs found
Comparing two ways of mixing PBMCs and washing medium by viability (A) and absolute count of live PBMCs (B).
<p>Paired samples 20 donors included. Ref.: reference group. NS: non-significant. **: p<0.01.</p
Impact of washing-medium temperature on viability (A) and PBMC recovery (B).
<p>20 donors included. Ref.: reference group. **: p<0.01.</p
Gating strategy to identify live/dead CD45+ leukocytes.
<p>A) Events were triggered on FSC-H at a deliberately low threshold to avoid accidental exclusion of dead cells. B) Doublet exclusion C) Identification of CD45 positive cells D) Dead cells identified as 7-AAD positive events. Compensation for spectral overlap was not required.</p
Centrifugation time and force and PBMC viability (A) and absolute live PBMC count (B).
<p>Paired samples from 18 donors included. Ref.: reference group. NS: non-significant. *: p<0.05. **: p<0.01.</p
PBMC viability (A) and absolute recovery (B) as a result of varying thawing time in 37°C heated water bath.
<p>Paired samples from 20 donors included. Ref.: reference group. NS: non-significant.</p
Temperature of centrifuge by PBMC viability (A) and absolute live PBMC recovery (B).
<p>Paired samples from 20 donors included. Ref.: reference group. NS: non-significant.</p
PBMC viability (A) and absolute live PBMC recovery (B) by incubation.
<p>Samples were stored in a 37°C incubator with 5% CO<sub>2</sub>. Paired samples from 20 donors were included. Ref.: reference group. NS: non-significant. *: p<0.05. **: p<0.01.</p
Distribution of maximum and mean storage time of red blood cell units transfused according to development of postoperative atrial fibrillation.
<p>Dashed lines represent the average storage time.</p
Baseline patient and operative characteristics.
<p>Baseline patient and operative characteristics.</p
Number of red blood cell units transfused on the day of surgery to each patient according to development of postoperative atrial fibrillation.
<p>Dashed line represents the average number of transfused units for each patient.</p