Principles and new trends in selection of root-nodule bacteria for inoculant production

The methodics summarize the basic information on applicability of soil bacteria for inoculation of field crops for purpose of nitrogen and phosphorus plant nutrition. Specific procedures of isolation, screening, selection and maintaining of root-nodule bacteria strains are described. The technology of inoculant production, quality control and inoculant appliation techniques are involved

Methodology of development and production of inoculants based on beneficial

The methodology summarizes the basic information on applicability of beneficial soil bacteria for field crop nutrition and maintaining soil fertility. Specific procedures of isolation, screening, selection and retention of bacterial strains are described. Part of the methodology is to describe the development and production of combined inoculats and their application in practice

Alternative phosphorus nutrition of plants

The methodology summarizes the basic information on applicability of phosphorus solubilizing bacteria for inoculation of field crops. Specific procedures of isolation, screening, selection and maintaining of bacterial strains are described. The technology of inoculant production, and application techniques are involved. The methodology offers the use of inoculants as an alternative plant nutrition. Results of practical experiments show that P-solubilizing bacteria increase the utilization of phosphorus. Plant growth promoting microorganisms in the inoculant also support the root system and consequently uptake of all nutrients by plants

Alternative nitrogen nutrition of plants

The methodology provides the basic information about the usability of nitrogenfixating bacteria. The methodology describes procedures of the isolation and screening of beneficial soil bacteria Azotobacter spp. and Rhizobium spp. It also provides recommendations to promote biological nitrogen fixation. The methodology also represents the use of inoculants as a necessary part of modern farming practices

Utilization and support of Azotobacter spp. for plant nutrition

The methodics summarizes the basic information on applicability of beneficial soil bacteria Azotobacter spp. for inoculation of field crops. Specific procedures of isolation, screening, selection and maintaining of bacterial strains are described. The technology of inoculant production, and application techniques are involved. The methodics includes evaluation and recommendation of specific farming practices supporting development and activity of Azotobacter spp

Evaluation of soil quality by some biological parameters

The methodology summarizes the basic information on applicability of biological methods for evaluation of soil quality. Selected biological methods are recommended as an early and sensitive indicators of contaminated soils and also as indicators with high sensitivity to long-term soil changes originated by management and environmental factors

Soil biological characteristics and microbial community structure in a field experiment

The influence of different fertilization treatments on soil biological characteristics and microbial community structure was investigated. Soil samples were taken from a long-term field experiment which was conducted to explore the effect of eight treatments: control (non-fertilized), NPK (nitrogen, phosphorus and potassium), FYM (farmyard manure), FYM + NPK, CSl + St (cattle slurry + straw), CSl + St + NPK, CSl, CSl + NPK. The highest values of invertase activity were found in treatment fertilized with farmyard manure combined mineral fertilizers, similarly to total N content, activity of urease, and C of microbial biomass. Dehydrogenase activity was lower in all treatments with mineral fertilization. Bacterial and actinobacterial T-RFLP profiles of 16S rRNA genes showed similar patterns in response to eight fertilization treatments. In both, the communities formed distinct groups, which were separated by organic fertilization i.e. cattle slurry and straw amendments along the x axes and by NPK amendments along the y axes using the Sammon’s method of multidimensional scaling. Significant correlations were determined in several situations related to diversity: between invertase and 1/Db (dominance, bacteria), 1/Da (dominance, actinobacteria) and between T-RFLP profiles of actinobacteria, nitrogen and organic carbon content and bacterial E (evenness)

The significance of conservation tillage technologies in crop production for soil fertility development

This methodology includes the newest knowledge on the influence of selected agricultural practices on soil properties and on soil fertility. It lays stress on the most important indicators of soil fertility that can help to evaluate the soil quality and it affords the instructions how to effectively increase the soil fertility and consequently plant production under use of conservation soil tillage technologies for field crop stand establishment